Aptamers based sensing of pregnancy associated glycoproteins (PAG) of bovine for early pregnancy detection

Abstract Tosyl activated magnetic beads were used for aptamer selection against PAG- 7 and 18 proteins of bovine origin. PAG proteins were immobilized on beads with further addition of biotin tagged aptamer library. The recognition of aptamers with PAG was identified by ST-HRP based approach which w...

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Autores principales:	Munna Lal Yadav, Abhishek Parashar, Nimai Charan Mahanandia, Vanya Bhushan, Sudarshan Kumar, Ashok Kumar Mohanty
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/921b33cb565244a5831c4206a198656f
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spelling	oai:doaj.org-article:921b33cb565244a5831c4206a198656f2021-12-05T12:13:14ZAptamers based sensing of pregnancy associated glycoproteins (PAG) of bovine for early pregnancy detection10.1038/s41598-021-02551-12045-2322https://doaj.org/article/921b33cb565244a5831c4206a198656f2021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02551-1https://doaj.org/toc/2045-2322Abstract Tosyl activated magnetic beads were used for aptamer selection against PAG- 7 and 18 proteins of bovine origin. PAG proteins were immobilized on beads with further addition of biotin tagged aptamer library. The recognition of aptamers with PAG was identified by ST-HRP based approach which was colorimetric in nature. The selected aptamers were sequenced and at the same time several new aptamers were identified. Later M-fold structure and G-quadruplex score of aptamers were analyzed for their selection. Those aptamers having high G value and complex structure were chosen. In dot blot assay, aptamers recognized PAG protein in an animal after 42 days of artificial insemination which later given birth to a healthy calf. Further the cross reactivity with serum of 0th day animal (post AI) or with non pregnant animal serum was minimal. Aptamers have also shown interaction with PAG protein of buffalo origin. These selected aptamers have commercial application especially in development of biosensors for early detection of pregnancy in bovine.Munna Lal YadavAbhishek ParasharNimai Charan MahanandiaVanya BhushanSudarshan KumarAshok Kumar MohantyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Munna Lal Yadav Abhishek Parashar Nimai Charan Mahanandia Vanya Bhushan Sudarshan Kumar Ashok Kumar Mohanty Aptamers based sensing of pregnancy associated glycoproteins (PAG) of bovine for early pregnancy detection
description	Abstract Tosyl activated magnetic beads were used for aptamer selection against PAG- 7 and 18 proteins of bovine origin. PAG proteins were immobilized on beads with further addition of biotin tagged aptamer library. The recognition of aptamers with PAG was identified by ST-HRP based approach which was colorimetric in nature. The selected aptamers were sequenced and at the same time several new aptamers were identified. Later M-fold structure and G-quadruplex score of aptamers were analyzed for their selection. Those aptamers having high G value and complex structure were chosen. In dot blot assay, aptamers recognized PAG protein in an animal after 42 days of artificial insemination which later given birth to a healthy calf. Further the cross reactivity with serum of 0th day animal (post AI) or with non pregnant animal serum was minimal. Aptamers have also shown interaction with PAG protein of buffalo origin. These selected aptamers have commercial application especially in development of biosensors for early detection of pregnancy in bovine.
format	article
author	Munna Lal Yadav Abhishek Parashar Nimai Charan Mahanandia Vanya Bhushan Sudarshan Kumar Ashok Kumar Mohanty
author_facet	Munna Lal Yadav Abhishek Parashar Nimai Charan Mahanandia Vanya Bhushan Sudarshan Kumar Ashok Kumar Mohanty
author_sort	Munna Lal Yadav
title	Aptamers based sensing of pregnancy associated glycoproteins (PAG) of bovine for early pregnancy detection
title_short	Aptamers based sensing of pregnancy associated glycoproteins (PAG) of bovine for early pregnancy detection
title_full	Aptamers based sensing of pregnancy associated glycoproteins (PAG) of bovine for early pregnancy detection
title_fullStr	Aptamers based sensing of pregnancy associated glycoproteins (PAG) of bovine for early pregnancy detection
title_full_unstemmed	Aptamers based sensing of pregnancy associated glycoproteins (PAG) of bovine for early pregnancy detection
title_sort	aptamers based sensing of pregnancy associated glycoproteins (pag) of bovine for early pregnancy detection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/921b33cb565244a5831c4206a198656f
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Aptamers based sensing of pregnancy associated glycoproteins (PAG) of bovine for early pregnancy detection

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