Identification of Rodent Homologs of Hepatitis C Virus and Pegiviruses

ABSTRACT Hepatitis C virus (HCV) and human pegivirus (HPgV or GB virus C) are globally distributed and infect 2 to 5% of the human population. The lack of tractable-animal models for these viruses, in particular for HCV, has hampered the study of infection, transmission, virulence, immunity, and pat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Amit Kapoor, Peter Simmonds, Troels K. H. Scheel, Brian Hjelle, John M. Cullen, Peter D. Burbelo, Lokendra V. Chauhan, Raja Duraisamy, Maria Sanchez Leon, Komal Jain, Kurt Jason Vandegrift, Charles H. Calisher, Charles M. Rice, W. Ian Lipkin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/921e00488ea046188be72ef946ac07cc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:921e00488ea046188be72ef946ac07cc
record_format	dspace
spelling	oai:doaj.org-article:921e00488ea046188be72ef946ac07cc2021-11-15T15:40:26ZIdentification of Rodent Homologs of Hepatitis C Virus and Pegiviruses10.1128/mBio.00216-132150-7511https://doaj.org/article/921e00488ea046188be72ef946ac07cc2013-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00216-13https://doaj.org/toc/2150-7511ABSTRACT Hepatitis C virus (HCV) and human pegivirus (HPgV or GB virus C) are globally distributed and infect 2 to 5% of the human population. The lack of tractable-animal models for these viruses, in particular for HCV, has hampered the study of infection, transmission, virulence, immunity, and pathogenesis. To address this challenge, we searched for homologous viruses in small mammals, including wild rodents. Here we report the discovery of several new hepaciviruses (HCV-like viruses) and pegiviruses (GB virus-like viruses) that infect wild rodents. Complete genome sequences were acquired for a rodent hepacivirus (RHV) found in Peromyscus maniculatus and a rodent pegivirus (RPgV) found in Neotoma albigula. Unique genomic features and phylogenetic analyses confirmed that these RHV and RPgV variants represent several novel virus species in the Hepacivirus and Pegivirus genera within the family Flaviviridae. The genetic diversity of the rodent hepaciviruses exceeded that observed for hepaciviruses infecting either humans or non-primates, leading to new insights into the origin, evolution, and host range of hepaciviruses. The presence of genes, encoded proteins, and translation elements homologous to those found in human hepaciviruses and pegiviruses suggests the potential for the development of new animal systems with which to model HCV pathogenesis, vaccine design, and treatment. IMPORTANCE The genetic and biological characterization of animal homologs of human viruses provides insights into the origins of human infections and enhances our ability to study their pathogenesis and explore preventive and therapeutic interventions. Horses are the only reported host of nonprimate homologs of hepatitis C virus (HCV). Here, we report the discovery of HCV-like viruses in wild rodents. The majority of HCV-like viruses were found in deer mice (Peromyscus maniculatus), a small rodent used in laboratories to study viruses, including hantaviruses. We also identified pegiviruses in rodents that are distinct from the pegiviruses found in primates, bats, and horses. These novel viruses may enable the development of small-animal models for HCV, the most common infectious cause of liver failure and hepatocellular carcinoma after hepatitis B virus, and help to explore the health relevance of the highly prevalent human pegiviruses.Amit KapoorPeter SimmondsTroels K. H. ScheelBrian HjelleJohn M. CullenPeter D. BurbeloLokendra V. ChauhanRaja DuraisamyMaria Sanchez LeonKomal JainKurt Jason VandegriftCharles H. CalisherCharles M. RiceW. Ian LipkinAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 2 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Amit Kapoor Peter Simmonds Troels K. H. Scheel Brian Hjelle John M. Cullen Peter D. Burbelo Lokendra V. Chauhan Raja Duraisamy Maria Sanchez Leon Komal Jain Kurt Jason Vandegrift Charles H. Calisher Charles M. Rice W. Ian Lipkin Identification of Rodent Homologs of Hepatitis C Virus and Pegiviruses
description	ABSTRACT Hepatitis C virus (HCV) and human pegivirus (HPgV or GB virus C) are globally distributed and infect 2 to 5% of the human population. The lack of tractable-animal models for these viruses, in particular for HCV, has hampered the study of infection, transmission, virulence, immunity, and pathogenesis. To address this challenge, we searched for homologous viruses in small mammals, including wild rodents. Here we report the discovery of several new hepaciviruses (HCV-like viruses) and pegiviruses (GB virus-like viruses) that infect wild rodents. Complete genome sequences were acquired for a rodent hepacivirus (RHV) found in Peromyscus maniculatus and a rodent pegivirus (RPgV) found in Neotoma albigula. Unique genomic features and phylogenetic analyses confirmed that these RHV and RPgV variants represent several novel virus species in the Hepacivirus and Pegivirus genera within the family Flaviviridae. The genetic diversity of the rodent hepaciviruses exceeded that observed for hepaciviruses infecting either humans or non-primates, leading to new insights into the origin, evolution, and host range of hepaciviruses. The presence of genes, encoded proteins, and translation elements homologous to those found in human hepaciviruses and pegiviruses suggests the potential for the development of new animal systems with which to model HCV pathogenesis, vaccine design, and treatment. IMPORTANCE The genetic and biological characterization of animal homologs of human viruses provides insights into the origins of human infections and enhances our ability to study their pathogenesis and explore preventive and therapeutic interventions. Horses are the only reported host of nonprimate homologs of hepatitis C virus (HCV). Here, we report the discovery of HCV-like viruses in wild rodents. The majority of HCV-like viruses were found in deer mice (Peromyscus maniculatus), a small rodent used in laboratories to study viruses, including hantaviruses. We also identified pegiviruses in rodents that are distinct from the pegiviruses found in primates, bats, and horses. These novel viruses may enable the development of small-animal models for HCV, the most common infectious cause of liver failure and hepatocellular carcinoma after hepatitis B virus, and help to explore the health relevance of the highly prevalent human pegiviruses.
format	article
author	Amit Kapoor Peter Simmonds Troels K. H. Scheel Brian Hjelle John M. Cullen Peter D. Burbelo Lokendra V. Chauhan Raja Duraisamy Maria Sanchez Leon Komal Jain Kurt Jason Vandegrift Charles H. Calisher Charles M. Rice W. Ian Lipkin
author_facet	Amit Kapoor Peter Simmonds Troels K. H. Scheel Brian Hjelle John M. Cullen Peter D. Burbelo Lokendra V. Chauhan Raja Duraisamy Maria Sanchez Leon Komal Jain Kurt Jason Vandegrift Charles H. Calisher Charles M. Rice W. Ian Lipkin
author_sort	Amit Kapoor
title	Identification of Rodent Homologs of Hepatitis C Virus and Pegiviruses
title_short	Identification of Rodent Homologs of Hepatitis C Virus and Pegiviruses
title_full	Identification of Rodent Homologs of Hepatitis C Virus and Pegiviruses
title_fullStr	Identification of Rodent Homologs of Hepatitis C Virus and Pegiviruses
title_full_unstemmed	Identification of Rodent Homologs of Hepatitis C Virus and Pegiviruses
title_sort	identification of rodent homologs of hepatitis c virus and pegiviruses
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/921e00488ea046188be72ef946ac07cc
work_keys_str_mv	AT amitkapoor identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT petersimmonds identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT troelskhscheel identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT brianhjelle identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT johnmcullen identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT peterdburbelo identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT lokendravchauhan identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT rajaduraisamy identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT mariasanchezleon identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT komaljain identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT kurtjasonvandegrift identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT charleshcalisher identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT charlesmrice identificationofrodenthomologsofhepatitiscvirusandpegiviruses AT wianlipkin identificationofrodenthomologsofhepatitiscvirusandpegiviruses
_version_	1718427779292200960

Identification of Rodent Homologs of Hepatitis C Virus and Pegiviruses

Ejemplares similares