The Small RNA ncS35 Regulates Growth in <named-content content-type="genus-species">Burkholderia cenocepacia</named-content> J2315

ABSTRACT Burkholderia cenocepacia J2315 is a member of the B. cepacia complex. It has a large genome with three replicons and one plasmid; 7,261 genes code for annotated proteins, while 113 code for functional RNAs. Small regulatory RNAs of B. cenocepacia have not yet been functionally characterized...

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Autores principales:	Sanne Kiekens, Andrea Sass, Filip Van Nieuwerburgh, Dieter Deforce, Tom Coenye
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Burkholderia cenocepacia metabolism small RNA Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/921fa85e9a234d9f90ebb4affb450020
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spelling	oai:doaj.org-article:921fa85e9a234d9f90ebb4affb4500202021-11-15T15:22:02ZThe Small RNA ncS35 Regulates Growth in <named-content content-type="genus-species">Burkholderia cenocepacia</named-content> J231510.1128/mSphere.00579-172379-5042https://doaj.org/article/921fa85e9a234d9f90ebb4affb4500202018-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00579-17https://doaj.org/toc/2379-5042ABSTRACT Burkholderia cenocepacia J2315 is a member of the B. cepacia complex. It has a large genome with three replicons and one plasmid; 7,261 genes code for annotated proteins, while 113 code for functional RNAs. Small regulatory RNAs of B. cenocepacia have not yet been functionally characterized. We investigated a small regulatory RNA, designated ncS35, that was discovered by differential RNA sequencing. Its expression under various conditions was quantified, and a deletion mutant, ΔncS35, was constructed. Compared to planktonic growth in a rich medium, the expression of ncS35 was elevated when B. cenocepacia J2315 was grown in biofilms and in minimal medium. Cells of the deletion mutant showed increased aggregation, higher metabolic activity, a higher growth rate, and an increased susceptibility to tobramycin. A transcriptomic analysis revealed upregulation of the phenylacetic acid and tryptophan degradation pathways in ΔncS35. Computational target prediction indicated that ncS35 likely interacts with the first gene of the tryptophan degradation pathway. Overall, we demonstrated that small RNA ncS35 is a noncoding RNA with an attenuating effect on the metabolic rate and growth. It is possible that slower growth protects B. cenocepacia J2315 against stressors acting on fast-dividing cells and enhances survival under unfavorable conditions. IMPORTANCE Small RNAs play an important role in the survival of bacteria in diverse environments. We explored the physiological role of ncS35, a small RNA expressed in B. cenocepacia J2315, an opportunistic pathogen in cystic fibrosis patients. In cystic fibrosis patients, infections can lead to “cepacia syndrome,” a rapidly progressing and often fatal pneumonia. Infections with Burkholderia spp. are difficult to threat with antibiotics because of their high intrinsic resistance and ability to form biofilms. We show that ncS35 attenuates the growth and reduces the metabolic rate of B. cenocepacia and influences biofilm structure. This demonstrates that as-yet-uncharacterized small RNAs with regulatory function can influence physiological traits of B. cenocepacia that are relevant for infection.Sanne KiekensAndrea SassFilip Van NieuwerburghDieter DeforceTom CoenyeAmerican Society for MicrobiologyarticleBurkholderia cenocepaciametabolismsmall RNAMicrobiologyQR1-502ENmSphere, Vol 3, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Burkholderia cenocepacia metabolism small RNA Microbiology QR1-502
spellingShingle	Burkholderia cenocepacia metabolism small RNA Microbiology QR1-502 Sanne Kiekens Andrea Sass Filip Van Nieuwerburgh Dieter Deforce Tom Coenye The Small RNA ncS35 Regulates Growth in <named-content content-type="genus-species">Burkholderia cenocepacia</named-content> J2315
description	ABSTRACT Burkholderia cenocepacia J2315 is a member of the B. cepacia complex. It has a large genome with three replicons and one plasmid; 7,261 genes code for annotated proteins, while 113 code for functional RNAs. Small regulatory RNAs of B. cenocepacia have not yet been functionally characterized. We investigated a small regulatory RNA, designated ncS35, that was discovered by differential RNA sequencing. Its expression under various conditions was quantified, and a deletion mutant, ΔncS35, was constructed. Compared to planktonic growth in a rich medium, the expression of ncS35 was elevated when B. cenocepacia J2315 was grown in biofilms and in minimal medium. Cells of the deletion mutant showed increased aggregation, higher metabolic activity, a higher growth rate, and an increased susceptibility to tobramycin. A transcriptomic analysis revealed upregulation of the phenylacetic acid and tryptophan degradation pathways in ΔncS35. Computational target prediction indicated that ncS35 likely interacts with the first gene of the tryptophan degradation pathway. Overall, we demonstrated that small RNA ncS35 is a noncoding RNA with an attenuating effect on the metabolic rate and growth. It is possible that slower growth protects B. cenocepacia J2315 against stressors acting on fast-dividing cells and enhances survival under unfavorable conditions. IMPORTANCE Small RNAs play an important role in the survival of bacteria in diverse environments. We explored the physiological role of ncS35, a small RNA expressed in B. cenocepacia J2315, an opportunistic pathogen in cystic fibrosis patients. In cystic fibrosis patients, infections can lead to “cepacia syndrome,” a rapidly progressing and often fatal pneumonia. Infections with Burkholderia spp. are difficult to threat with antibiotics because of their high intrinsic resistance and ability to form biofilms. We show that ncS35 attenuates the growth and reduces the metabolic rate of B. cenocepacia and influences biofilm structure. This demonstrates that as-yet-uncharacterized small RNAs with regulatory function can influence physiological traits of B. cenocepacia that are relevant for infection.
format	article
author	Sanne Kiekens Andrea Sass Filip Van Nieuwerburgh Dieter Deforce Tom Coenye
author_facet	Sanne Kiekens Andrea Sass Filip Van Nieuwerburgh Dieter Deforce Tom Coenye
author_sort	Sanne Kiekens
title	The Small RNA ncS35 Regulates Growth in <named-content content-type="genus-species">Burkholderia cenocepacia</named-content> J2315
title_short	The Small RNA ncS35 Regulates Growth in <named-content content-type="genus-species">Burkholderia cenocepacia</named-content> J2315
title_full	The Small RNA ncS35 Regulates Growth in <named-content content-type="genus-species">Burkholderia cenocepacia</named-content> J2315
title_fullStr	The Small RNA ncS35 Regulates Growth in <named-content content-type="genus-species">Burkholderia cenocepacia</named-content> J2315
title_full_unstemmed	The Small RNA ncS35 Regulates Growth in <named-content content-type="genus-species">Burkholderia cenocepacia</named-content> J2315
title_sort	small rna ncs35 regulates growth in <named-content content-type="genus-species">burkholderia cenocepacia</named-content> j2315
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/921fa85e9a234d9f90ebb4affb450020
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The Small RNA ncS35 Regulates Growth in <named-content content-type="genus-species">Burkholderia cenocepacia</named-content> J2315

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