Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure

Abstract Linx is a member of the leucine-rich repeat and immunoglobulin family of membrane proteins which has critical roles in the development of the peripheral nervous system and forebrain connectivity. A previous study showed that Linx is expressed in projection neurons in the cortex and in cells...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shaniya Abudureyimu, Naoya Asai, Atsushi Enomoto, Liang Weng, Hiroki Kobayashi, Xiaoze Wang, Chen Chen, Shinji Mii, Masahide Takahashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9220edffbbd24b4f971216f607abc8b5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9220edffbbd24b4f971216f607abc8b5
record_format	dspace
spelling	oai:doaj.org-article:9220edffbbd24b4f971216f607abc8b52021-12-02T12:32:35ZEssential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure10.1038/s41598-018-24064-02045-2322https://doaj.org/article/9220edffbbd24b4f971216f607abc8b52018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24064-0https://doaj.org/toc/2045-2322Abstract Linx is a member of the leucine-rich repeat and immunoglobulin family of membrane proteins which has critical roles in the development of the peripheral nervous system and forebrain connectivity. A previous study showed that Linx is expressed in projection neurons in the cortex and in cells that comprise the passage to the prethalamus that form the internal capsule, indicating the involvement of Linx in axon guidance and cell-cell communication. In this study, we found that Linx-deficient mice develop severe hydrocephalus and die perinatally by unknown mechanisms. Importantly, mice heterozygous for the linx gene exhibited defects in the development of the anterior commissure in addition to hydrocephalus, indicating haploinsufficiency of the linx gene in forebrain development. In N1E-115 neuroblastoma cells and primary cultured hippocampal neurons, Linx depletion led to impaired neurite extension and an increase in cell body size. Consistent with this, but of unknown significance, we found that Linx interacts with and upregulates the activity of Rho-kinase, a modulator of many cellular processes including cytoskeletal organization. These data suggest a role for Linx in the regulation of complex forebrain connectivity, and future identification of its extracellular ligand(s) will help clarify this function.Shaniya AbudureyimuNaoya AsaiAtsushi EnomotoLiang WengHiroki KobayashiXiaoze WangChen ChenShinji MiiMasahide TakahashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shaniya Abudureyimu Naoya Asai Atsushi Enomoto Liang Weng Hiroki Kobayashi Xiaoze Wang Chen Chen Shinji Mii Masahide Takahashi Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure
description	Abstract Linx is a member of the leucine-rich repeat and immunoglobulin family of membrane proteins which has critical roles in the development of the peripheral nervous system and forebrain connectivity. A previous study showed that Linx is expressed in projection neurons in the cortex and in cells that comprise the passage to the prethalamus that form the internal capsule, indicating the involvement of Linx in axon guidance and cell-cell communication. In this study, we found that Linx-deficient mice develop severe hydrocephalus and die perinatally by unknown mechanisms. Importantly, mice heterozygous for the linx gene exhibited defects in the development of the anterior commissure in addition to hydrocephalus, indicating haploinsufficiency of the linx gene in forebrain development. In N1E-115 neuroblastoma cells and primary cultured hippocampal neurons, Linx depletion led to impaired neurite extension and an increase in cell body size. Consistent with this, but of unknown significance, we found that Linx interacts with and upregulates the activity of Rho-kinase, a modulator of many cellular processes including cytoskeletal organization. These data suggest a role for Linx in the regulation of complex forebrain connectivity, and future identification of its extracellular ligand(s) will help clarify this function.
format	article
author	Shaniya Abudureyimu Naoya Asai Atsushi Enomoto Liang Weng Hiroki Kobayashi Xiaoze Wang Chen Chen Shinji Mii Masahide Takahashi
author_facet	Shaniya Abudureyimu Naoya Asai Atsushi Enomoto Liang Weng Hiroki Kobayashi Xiaoze Wang Chen Chen Shinji Mii Masahide Takahashi
author_sort	Shaniya Abudureyimu
title	Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure
title_short	Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure
title_full	Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure
title_fullStr	Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure
title_full_unstemmed	Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure
title_sort	essential role of linx/islr2 in the development of the forebrain anterior commissure
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9220edffbbd24b4f971216f607abc8b5
work_keys_str_mv	AT shaniyaabudureyimu essentialroleoflinxislr2inthedevelopmentoftheforebrainanteriorcommissure AT naoyaasai essentialroleoflinxislr2inthedevelopmentoftheforebrainanteriorcommissure AT atsushienomoto essentialroleoflinxislr2inthedevelopmentoftheforebrainanteriorcommissure AT liangweng essentialroleoflinxislr2inthedevelopmentoftheforebrainanteriorcommissure AT hirokikobayashi essentialroleoflinxislr2inthedevelopmentoftheforebrainanteriorcommissure AT xiaozewang essentialroleoflinxislr2inthedevelopmentoftheforebrainanteriorcommissure AT chenchen essentialroleoflinxislr2inthedevelopmentoftheforebrainanteriorcommissure AT shinjimii essentialroleoflinxislr2inthedevelopmentoftheforebrainanteriorcommissure AT masahidetakahashi essentialroleoflinxislr2inthedevelopmentoftheforebrainanteriorcommissure
_version_	1718394048470843392

Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure

Ejemplares similares