Effects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages

The present study was aimed at investigating the effects of four LBP fractions with different molecular weights (MWs), designated LBP2, LBP3, LBP4 and LBP5, on RAW264.7 macrophages function. Results showed that LBP fractions could significantly enhance the expression of CD86 and MHC-II molecules on...

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Autores principales:	Xiangliang Deng, Qi Liu, Yajun Fu, Xia Luo, Minghua Hu, Fangli Ma, Qing Wang, Xiaoping Lai, Lian Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	lycium barbarum polysaccharide molecular weight macrophages griess reagent flow cytometer Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9222cf8ca1d940c4ac6405ec65d4646b
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spelling	oai:doaj.org-article:9222cf8ca1d940c4ac6405ec65d4646b2021-11-26T11:19:46ZEffects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages0954-01051465-344310.1080/09540105.2018.1457628https://doaj.org/article/9222cf8ca1d940c4ac6405ec65d4646b2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1457628https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443The present study was aimed at investigating the effects of four LBP fractions with different molecular weights (MWs), designated LBP2, LBP3, LBP4 and LBP5, on RAW264.7 macrophages function. Results showed that LBP fractions could significantly enhance the expression of CD86 and MHC-II molecules on RAW264.7 macrophages. LBP3, LBP4 and LBP5 could enhance the production of ROS, NO, TNF-α and IL-6, and the phagocytosis of RAW264.7 macrophages. LBP2 with an MW of larger than 350 kDa could only enhance the secretion of TNF-α. LBP3 enhanced the RAW264.7 macrophages function in a dose-dependent manner and also enhanced the iNOS mRNA expression in the cells. These results demonstrated that the immunomodulatory activity of LBP on RAW264.7 macrophages was closely related to its MW. It indicated that fractions with an MW smaller than 350 kDa were the main active fractions of LBP in enhancing macrophages function.Xiangliang DengQi LiuYajun FuXia LuoMinghua HuFangli MaQing WangXiaoping LaiLian ZhouTaylor & Francis Grouparticlelycium barbarum polysaccharidemolecular weightmacrophagesgriess reagentflow cytometerAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 808-820 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	lycium barbarum polysaccharide molecular weight macrophages griess reagent flow cytometer Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	lycium barbarum polysaccharide molecular weight macrophages griess reagent flow cytometer Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Xiangliang Deng Qi Liu Yajun Fu Xia Luo Minghua Hu Fangli Ma Qing Wang Xiaoping Lai Lian Zhou Effects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages
description	The present study was aimed at investigating the effects of four LBP fractions with different molecular weights (MWs), designated LBP2, LBP3, LBP4 and LBP5, on RAW264.7 macrophages function. Results showed that LBP fractions could significantly enhance the expression of CD86 and MHC-II molecules on RAW264.7 macrophages. LBP3, LBP4 and LBP5 could enhance the production of ROS, NO, TNF-α and IL-6, and the phagocytosis of RAW264.7 macrophages. LBP2 with an MW of larger than 350 kDa could only enhance the secretion of TNF-α. LBP3 enhanced the RAW264.7 macrophages function in a dose-dependent manner and also enhanced the iNOS mRNA expression in the cells. These results demonstrated that the immunomodulatory activity of LBP on RAW264.7 macrophages was closely related to its MW. It indicated that fractions with an MW smaller than 350 kDa were the main active fractions of LBP in enhancing macrophages function.
format	article
author	Xiangliang Deng Qi Liu Yajun Fu Xia Luo Minghua Hu Fangli Ma Qing Wang Xiaoping Lai Lian Zhou
author_facet	Xiangliang Deng Qi Liu Yajun Fu Xia Luo Minghua Hu Fangli Ma Qing Wang Xiaoping Lai Lian Zhou
author_sort	Xiangliang Deng
title	Effects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages
title_short	Effects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages
title_full	Effects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages
title_fullStr	Effects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages
title_full_unstemmed	Effects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages
title_sort	effects of lycium barbarum polysaccharides with different molecular weights on function of raw264.7 macrophages
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/9222cf8ca1d940c4ac6405ec65d4646b
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Effects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages

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