Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish
Abraham Heriberto Garcia Campoy,1 Rosa Martha Perez Gutierrez,1 Gabriela Manriquez-Alvirde,2 Alethia Muñiz Ramirez3 1Laboratorio de Investigación de Productos Naturales, Escuela Superior de Ingenieria Quimica e Industrias extractivas IPN, Unidad Profesional Adolfo Lopez Mateos...
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Dove Medical Press
2018
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oai:doaj.org-article:92263f2d81074b1397213f27bbae83352021-12-02T08:49:58ZProtection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish1178-2013https://doaj.org/article/92263f2d81074b1397213f27bbae83352018-05-01T00:00:00Zhttps://www.dovepress.com/protection-of-silver-nanoparticles-using-eysenhardtia-polystachya-in-p-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Abraham Heriberto Garcia Campoy,1 Rosa Martha Perez Gutierrez,1 Gabriela Manriquez-Alvirde,2 Alethia Muñiz Ramirez3 1Laboratorio de Investigación de Productos Naturales, Escuela Superior de Ingenieria Quimica e Industrias extractivas IPN, Unidad Profesional Adolfo Lopez Mateos, Mexico City, Mexico; 2Universidad Autonoma Metropolitana, Mexico City, Mexico; 3CONACYT-IPICYT/CIIDZA, San Luis Potosí, México Background: The aim was to explore the efficacy of extract of Eysenhardtia polystachya-loaded silver nanoparticles (EP/AgNPs) on pancreatic β cells, INS-1 cells, and zebrafish as a valuable model for the study of diabetes mellitus.Materials and methods: EP/AgNPs were synthesized using methanol/water bark extract of E. polystachya and characterized using various physicochemical techniques.Results: Immersion of adult zebrafish in 111 mM glucose solution resulted in a sustained hyperglycemic, hyperlipidemic state, and serum insulin levels decreased. The synthesized EP/AgNPs showed an absorption peak at 413 nm on ultraviolet–visible spectroscopy, revealing the surface plasmon resonance of the nanoparticles. Transmission electron microscopy indicated that most of the particles were spherical, with a diameter of 10–12 nm, a polydispersity index of 0.197, and a zeta potential of -32.25 mV, suggesting high stability of the nanoparticles. EP/AgNPs promote pancreatic β-cell survival, insulin secretion, enhanced hyperglycemia, and hyperlipidemia in glucose-induced diabetic zebrafish. EP/AgNPs also showed protection of the pancreatic β-cell line INS-1 against hydrogen peroxide-induced oxidative injury.Conclusion: The results indicate that EP/AgNPs have good antidiabetic activity and therefore could be used to prevent the development of diabetes. Keywords: Eysenhardtia polystachya, zebrafish, silver nanoparticles, diabetes, insulin, hyperlipidemiaGarcia Campoy AHPerez Gutierrez RMManriquez-Alvirde GMuñiz Ramirez ADove Medical PressarticleEysenhardtia polystachyazebrafishsilver nanoparticlesdiabetesinsulinhyperlipidemiaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 2601-2612 (2018) |
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Eysenhardtia polystachya zebrafish silver nanoparticles diabetes insulin hyperlipidemia Medicine (General) R5-920 |
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Eysenhardtia polystachya zebrafish silver nanoparticles diabetes insulin hyperlipidemia Medicine (General) R5-920 Garcia Campoy AH Perez Gutierrez RM Manriquez-Alvirde G Muñiz Ramirez A Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish |
| description |
Abraham Heriberto Garcia Campoy,1 Rosa Martha Perez Gutierrez,1 Gabriela Manriquez-Alvirde,2 Alethia Muñiz Ramirez3 1Laboratorio de Investigación de Productos Naturales, Escuela Superior de Ingenieria Quimica e Industrias extractivas IPN, Unidad Profesional Adolfo Lopez Mateos, Mexico City, Mexico; 2Universidad Autonoma Metropolitana, Mexico City, Mexico; 3CONACYT-IPICYT/CIIDZA, San Luis Potosí, México Background: The aim was to explore the efficacy of extract of Eysenhardtia polystachya-loaded silver nanoparticles (EP/AgNPs) on pancreatic β cells, INS-1 cells, and zebrafish as a valuable model for the study of diabetes mellitus.Materials and methods: EP/AgNPs were synthesized using methanol/water bark extract of E. polystachya and characterized using various physicochemical techniques.Results: Immersion of adult zebrafish in 111 mM glucose solution resulted in a sustained hyperglycemic, hyperlipidemic state, and serum insulin levels decreased. The synthesized EP/AgNPs showed an absorption peak at 413 nm on ultraviolet–visible spectroscopy, revealing the surface plasmon resonance of the nanoparticles. Transmission electron microscopy indicated that most of the particles were spherical, with a diameter of 10–12 nm, a polydispersity index of 0.197, and a zeta potential of -32.25 mV, suggesting high stability of the nanoparticles. EP/AgNPs promote pancreatic β-cell survival, insulin secretion, enhanced hyperglycemia, and hyperlipidemia in glucose-induced diabetic zebrafish. EP/AgNPs also showed protection of the pancreatic β-cell line INS-1 against hydrogen peroxide-induced oxidative injury.Conclusion: The results indicate that EP/AgNPs have good antidiabetic activity and therefore could be used to prevent the development of diabetes. Keywords: Eysenhardtia polystachya, zebrafish, silver nanoparticles, diabetes, insulin, hyperlipidemia |
| format |
article |
| author |
Garcia Campoy AH Perez Gutierrez RM Manriquez-Alvirde G Muñiz Ramirez A |
| author_facet |
Garcia Campoy AH Perez Gutierrez RM Manriquez-Alvirde G Muñiz Ramirez A |
| author_sort |
Garcia Campoy AH |
| title |
Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish |
| title_short |
Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish |
| title_full |
Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish |
| title_fullStr |
Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish |
| title_full_unstemmed |
Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish |
| title_sort |
protection of silver nanoparticles using eysenhardtia polystachya in peroxide-induced pancreatic β-cell damage and their antidiabetic properties in zebrafish |
| publisher |
Dove Medical Press |
| publishDate |
2018 |
| url |
https://doaj.org/article/92263f2d81074b1397213f27bbae8335 |
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