Next-Generation Sequencing Combined with Specific PCR Assays To Determine the Bacterial 16S rRNA Gene Profiles of Middle Ear Fluid Collected from Children with Acute Otitis Media

ABSTRACT The aim of the study was to analyze the bacteriome of acute otitis media with a novel modification of next-generation sequencing techniques. Outpatient children with acute otitis media were enrolled in the study, and middle ear fluids were collected during 90 episodes from 79 subjects aged...

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Bibliographic Details
Main Authors:	Saara Sillanpää, Lenka Kramna, Sami Oikarinen, Markku Sipilä, Markus Rautiainen, Janne Aittoniemi, Jussi Laranne, Heikki Hyöty, Ondrej Cinek
Format:	article
Language:	EN
Published:	American Society for Microbiology 2017
Subjects:	16S profiling acute otitis media bacteriome profiling mass sequencing next-generation sequencing Microbiology QR1-502
Online Access:	https://doaj.org/article/9237ed312b284cc7901c3b10d5292563
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Next-Generation Sequencing Combined with Specific PCR Assays To Determine the Bacterial 16S rRNA Gene Profiles of Middle Ear Fluid Collected from Children with Acute Otitis Media

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