Next-Generation Sequencing Combined with Specific PCR Assays To Determine the Bacterial 16S rRNA Gene Profiles of Middle Ear Fluid Collected from Children with Acute Otitis Media

Next-Generation Sequencing Combined with Specific PCR Assays To Determine the Bacterial 16S rRNA Gene Profiles of Middle Ear Fluid Collected from Children with Acute Otitis Media

ABSTRACT The aim of the study was to analyze the bacteriome of acute otitis media with a novel modification of next-generation sequencing techniques. Outpatient children with acute otitis media were enrolled in the study, and middle ear fluids were collected during 90 episodes from 79 subjects aged...

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Auteurs principaux: Saara Sillanpää, Lenka Kramna, Sami Oikarinen, Markku Sipilä, Markus Rautiainen, Janne Aittoniemi, Jussi Laranne, Heikki Hyöty, Ondrej Cinek
Format: article
Langue:EN
Publié: American Society for Microbiology 2017
Sujets:
16S profiling
acute otitis media
bacteriome profiling
mass sequencing
next-generation sequencing
Microbiology
QR1-502
Accès en ligne:https://doaj.org/article/9237ed312b284cc7901c3b10d5292563
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https://doaj.org/article/9237ed312b284cc7901c3b10d5292563

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