Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists

Abstract Periodontal ligament stem cells similarly to the mesenchymal stem cells of other tissues possess immunomodulatory properties, which are regulated by different cytokines, particularly by interferon-γ (IFN-γ). In contrast, less information is provided about the effect of toll-like receptors l...

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Autores principales:	Oleh Andrukhov, Johanna Sang-A Hong, Olena Andrukhova, Alice Blufstein, Andreas Moritz, Xiaohui Rausch-Fan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/923c9838a21b48bba571a544cb9ffa14
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spelling	oai:doaj.org-article:923c9838a21b48bba571a544cb9ffa142021-12-02T15:05:31ZResponse of human periodontal ligament stem cells to IFN-γ and TLR-agonists10.1038/s41598-017-12480-72045-2322https://doaj.org/article/923c9838a21b48bba571a544cb9ffa142017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-12480-7https://doaj.org/toc/2045-2322Abstract Periodontal ligament stem cells similarly to the mesenchymal stem cells of other tissues possess immunomodulatory properties, which are regulated by different cytokines, particularly by interferon-γ (IFN-γ). In contrast, less information is provided about the effect of toll-like receptors ligand on immunomodulatory properties of these cells. In the present study we investigated the response of human periodontal ligament stem cells (hPDLSCs) in response to simultaneous stimulation with IFN-γ and toll-like receptor (TLR) agonists. The resulting expression of indoleamine-2,3-dioxygenase-1 (IDO-1), interleukin (IL)-6, IL-8 and monocyte chemotactic protein 1 (MCP-1) was investigated. The expression of IDO-1 was upregulated by IFN-γ in both gene and protein levels. TLR2 agonists Pam3CSK4 induced gene expression of IDO-1, but had no effect on protein expression. IFN-γ induced IDO-1 protein expression was further enhanced by Pam3CSK4. TLR-4 agonist E. coli LPS has no significant effect on neither basal nor IFN-γ induced IDO-1 protein expression. The production of IL-6, IL-8, and MCP-1 was induced by TLR agonists. Neither basal nor TLR agonists induced production of these proteins was affected by IFN-γ. Our data shows potential interaction between IFN-γ and TLR2 responses in hPDLSCs, which might be involved in regulation of immune response in inflammatory diseases, and particularly periodontitis.Oleh AndrukhovJohanna Sang-A HongOlena AndrukhovaAlice BlufsteinAndreas MoritzXiaohui Rausch-FanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oleh Andrukhov Johanna Sang-A Hong Olena Andrukhova Alice Blufstein Andreas Moritz Xiaohui Rausch-Fan Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists
description	Abstract Periodontal ligament stem cells similarly to the mesenchymal stem cells of other tissues possess immunomodulatory properties, which are regulated by different cytokines, particularly by interferon-γ (IFN-γ). In contrast, less information is provided about the effect of toll-like receptors ligand on immunomodulatory properties of these cells. In the present study we investigated the response of human periodontal ligament stem cells (hPDLSCs) in response to simultaneous stimulation with IFN-γ and toll-like receptor (TLR) agonists. The resulting expression of indoleamine-2,3-dioxygenase-1 (IDO-1), interleukin (IL)-6, IL-8 and monocyte chemotactic protein 1 (MCP-1) was investigated. The expression of IDO-1 was upregulated by IFN-γ in both gene and protein levels. TLR2 agonists Pam3CSK4 induced gene expression of IDO-1, but had no effect on protein expression. IFN-γ induced IDO-1 protein expression was further enhanced by Pam3CSK4. TLR-4 agonist E. coli LPS has no significant effect on neither basal nor IFN-γ induced IDO-1 protein expression. The production of IL-6, IL-8, and MCP-1 was induced by TLR agonists. Neither basal nor TLR agonists induced production of these proteins was affected by IFN-γ. Our data shows potential interaction between IFN-γ and TLR2 responses in hPDLSCs, which might be involved in regulation of immune response in inflammatory diseases, and particularly periodontitis.
format	article
author	Oleh Andrukhov Johanna Sang-A Hong Olena Andrukhova Alice Blufstein Andreas Moritz Xiaohui Rausch-Fan
author_facet	Oleh Andrukhov Johanna Sang-A Hong Olena Andrukhova Alice Blufstein Andreas Moritz Xiaohui Rausch-Fan
author_sort	Oleh Andrukhov
title	Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists
title_short	Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists
title_full	Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists
title_fullStr	Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists
title_full_unstemmed	Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists
title_sort	response of human periodontal ligament stem cells to ifn-γ and tlr-agonists
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/923c9838a21b48bba571a544cb9ffa14
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Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists

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