Genetic characterization and zoonotic potential of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, Southwest China

Blastocystis is a prevalent eukaryotic parasite that has been identified in a wide range of hosts. Several species are considered potential sources of Blastocystis infection in humans, but little is known about the prevalence of Blastocystis in wild animals. In this study, the prevalence and subtype...

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Autores principales:	Chen Shanyu, Meng Wanyu, Zhou Ziyao, Deng Lei, Shi Xiaogang, Chai Yijun, Liu Haifeng, Cheng Yuehong, Zhong Zhijun, Fu Hualin, Shen Liuhong, Zhang Kun, He Tingmei, Peng Guangneng
Formato:	article
Lenguaje:	EN
Publicado:	EDP Sciences 2021
Materias:	blastocystis wild animals prevalence subtypes china Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/9240d58d6c66443e80e362005750e733
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spelling	oai:doaj.org-article:9240d58d6c66443e80e362005750e7332021-11-08T15:21:45ZGenetic characterization and zoonotic potential of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, Southwest China1776-104210.1051/parasite/2021071https://doaj.org/article/9240d58d6c66443e80e362005750e7332021-01-01T00:00:00Zhttps://www.parasite-journal.org/articles/parasite/full_html/2021/01/parasite210099/parasite210099.htmlhttps://doaj.org/toc/1776-1042Blastocystis is a prevalent eukaryotic parasite that has been identified in a wide range of hosts. Several species are considered potential sources of Blastocystis infection in humans, but little is known about the prevalence of Blastocystis in wild animals. In this study, the prevalence and subtypes of Blastocystis were investigated to assess the zoonotic potential of wild animals in Sichuan Wolong National Natural Reserve. A total of 300 fecal samples were collected from 27 wildlife species in three areas of the Reserve. The subtype (ST), genetic characteristics, and prevalence of Blastocystis were determined by PCR amplification of part (~600 bp) of the SSU rRNA gene. Thirty fecal samples (10.0%) were Blastocystis-positive. The highest prevalence of Blastocystis was found in Yinchanggou (18.3%), with significantly less found in Niutoushan (7.5%) and Genda (5.5%) (p < 0.05). No significant differences were associated with different orders of animals in prevalence, which may be because of the small number of positive samples obtained. Sequence analysis showed five subtypes (ST1, ST3, ST5, ST13, and ST14), with ST13 and ST14 being predominant (33% each), followed by ST1 (20%). This is the first molecular investigation of Blastocystis infection in the wild animals of southwestern China. Subtypes ST1, ST3, ST5, and ST14 have previously been identified in humans, suggesting that wild animals may be potential reservoirs of Blastocystis for humans.Chen ShanyuMeng WanyuZhou ZiyaoDeng LeiShi XiaogangChai YijunLiu HaifengCheng YuehongZhong ZhijunFu HualinShen LiuhongZhang KunHe TingmeiPeng GuangnengEDP Sciencesarticleblastocystiswild animalsprevalencesubtypeschinaInfectious and parasitic diseasesRC109-216ENParasite, Vol 28, p 73 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	blastocystis wild animals prevalence subtypes china Infectious and parasitic diseases RC109-216
spellingShingle	blastocystis wild animals prevalence subtypes china Infectious and parasitic diseases RC109-216 Chen Shanyu Meng Wanyu Zhou Ziyao Deng Lei Shi Xiaogang Chai Yijun Liu Haifeng Cheng Yuehong Zhong Zhijun Fu Hualin Shen Liuhong Zhang Kun He Tingmei Peng Guangneng Genetic characterization and zoonotic potential of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, Southwest China
description	Blastocystis is a prevalent eukaryotic parasite that has been identified in a wide range of hosts. Several species are considered potential sources of Blastocystis infection in humans, but little is known about the prevalence of Blastocystis in wild animals. In this study, the prevalence and subtypes of Blastocystis were investigated to assess the zoonotic potential of wild animals in Sichuan Wolong National Natural Reserve. A total of 300 fecal samples were collected from 27 wildlife species in three areas of the Reserve. The subtype (ST), genetic characteristics, and prevalence of Blastocystis were determined by PCR amplification of part (~600 bp) of the SSU rRNA gene. Thirty fecal samples (10.0%) were Blastocystis-positive. The highest prevalence of Blastocystis was found in Yinchanggou (18.3%), with significantly less found in Niutoushan (7.5%) and Genda (5.5%) (p < 0.05). No significant differences were associated with different orders of animals in prevalence, which may be because of the small number of positive samples obtained. Sequence analysis showed five subtypes (ST1, ST3, ST5, ST13, and ST14), with ST13 and ST14 being predominant (33% each), followed by ST1 (20%). This is the first molecular investigation of Blastocystis infection in the wild animals of southwestern China. Subtypes ST1, ST3, ST5, and ST14 have previously been identified in humans, suggesting that wild animals may be potential reservoirs of Blastocystis for humans.
format	article
author	Chen Shanyu Meng Wanyu Zhou Ziyao Deng Lei Shi Xiaogang Chai Yijun Liu Haifeng Cheng Yuehong Zhong Zhijun Fu Hualin Shen Liuhong Zhang Kun He Tingmei Peng Guangneng
author_facet	Chen Shanyu Meng Wanyu Zhou Ziyao Deng Lei Shi Xiaogang Chai Yijun Liu Haifeng Cheng Yuehong Zhong Zhijun Fu Hualin Shen Liuhong Zhang Kun He Tingmei Peng Guangneng
author_sort	Chen Shanyu
title	Genetic characterization and zoonotic potential of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, Southwest China
title_short	Genetic characterization and zoonotic potential of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, Southwest China
title_full	Genetic characterization and zoonotic potential of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, Southwest China
title_fullStr	Genetic characterization and zoonotic potential of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, Southwest China
title_full_unstemmed	Genetic characterization and zoonotic potential of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, Southwest China
title_sort	genetic characterization and zoonotic potential of blastocystis from wild animals in sichuan wolong national natural reserve, southwest china
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/9240d58d6c66443e80e362005750e733
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Genetic characterization and zoonotic potential of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, Southwest China

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