Protective Effects of Lactobacillus plantarum Lac16 on Clostridium perfringens Infection-Associated Injury in IPEC-J2 Cells

Clostridium perfringens (C. perfringens) causes intestinal injury through overgrowth and the secretion of multiple toxins, leading to diarrhea and necrotic enteritis in animals, including pigs, chickens, and sheep. This study aimed to investigate the protective...

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Autores principales:	Yuanhao Zhou, Baikui Wang, Qi Wang, Li Tang, Peng Zou, Zihan Zeng, Huihua Zhang, Li Gong, Weifen Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Lactobacillus plantarum</i> <i>Clostridium perfringens</i> cellular injury intestinal barrier integrity inflammation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/92448e1e2191427fa8d09d2539f7d829
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spelling	oai:doaj.org-article:92448e1e2191427fa8d09d2539f7d8292021-11-25T17:56:08ZProtective Effects of <i>Lactobacillus plantarum</i> Lac16 on <i>Clostridium perfringens</i> Infection-Associated Injury in IPEC-J2 Cells10.3390/ijms2222123881422-00671661-6596https://doaj.org/article/92448e1e2191427fa8d09d2539f7d8292021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12388https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<i>Clostridium perfringens</i> (<i>C. perfringens</i>) causes intestinal injury through overgrowth and the secretion of multiple toxins, leading to diarrhea and necrotic enteritis in animals, including pigs, chickens, and sheep. This study aimed to investigate the protective effects of <i>Lactobacillus plantarum</i> (<i>L. plantarum</i>) Lac16 on <i>C. perfringens</i> infection-associated injury in intestinal porcine epithelial cell line (IPEC-J2). The results showed that <i>L. plantarum</i> Lac16 significantly inhibited the growth of <i>C. perfringens</i>, which was accompanied by a decrease in pH levels. In addition, <i>L. plantarum</i> Lac16 significantly elevated the mRNA expression levels of host defense peptides (HDPs) in IPEC-J2 cells, decreased the adhesion of <i>C. perfringens</i> to IPEC-J2 cells, and attenuated <i>C. perfringens</i>-induced cellular cytotoxicity and intestinal barrier damage. Furthermore, <i>L. plantarum</i> Lac16 significantly suppressed <i>C. perfringens</i>-induced gene expressions of proinflammatory cytokines and pattern recognition receptors (PRRs) in IPEC-J2 cells. Moreover, <i>L. plantarum</i> Lac16 preincubation effectively inhibited the phosphorylation of p65 caused by <i>C. perfringens</i> infection. Collectively, probiotic <i>L. plantarum</i> Lac16 exerts protective effects against <i>C. perfringens</i> infection-associated injury in IPEC-J2 cells.Yuanhao ZhouBaikui WangQi WangLi TangPeng ZouZihan ZengHuihua ZhangLi GongWeifen LiMDPI AGarticle<i>Lactobacillus plantarum</i><i>Clostridium perfringens</i>cellular injuryintestinal barrier integrityinflammationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12388, p 12388 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Lactobacillus plantarum</i> <i>Clostridium perfringens</i> cellular injury intestinal barrier integrity inflammation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Lactobacillus plantarum</i> <i>Clostridium perfringens</i> cellular injury intestinal barrier integrity inflammation Biology (General) QH301-705.5 Chemistry QD1-999 Yuanhao Zhou Baikui Wang Qi Wang Li Tang Peng Zou Zihan Zeng Huihua Zhang Li Gong Weifen Li Protective Effects of <i>Lactobacillus plantarum</i> Lac16 on <i>Clostridium perfringens</i> Infection-Associated Injury in IPEC-J2 Cells
description	<i>Clostridium perfringens</i> (<i>C. perfringens</i>) causes intestinal injury through overgrowth and the secretion of multiple toxins, leading to diarrhea and necrotic enteritis in animals, including pigs, chickens, and sheep. This study aimed to investigate the protective effects of <i>Lactobacillus plantarum</i> (<i>L. plantarum</i>) Lac16 on <i>C. perfringens</i> infection-associated injury in intestinal porcine epithelial cell line (IPEC-J2). The results showed that <i>L. plantarum</i> Lac16 significantly inhibited the growth of <i>C. perfringens</i>, which was accompanied by a decrease in pH levels. In addition, <i>L. plantarum</i> Lac16 significantly elevated the mRNA expression levels of host defense peptides (HDPs) in IPEC-J2 cells, decreased the adhesion of <i>C. perfringens</i> to IPEC-J2 cells, and attenuated <i>C. perfringens</i>-induced cellular cytotoxicity and intestinal barrier damage. Furthermore, <i>L. plantarum</i> Lac16 significantly suppressed <i>C. perfringens</i>-induced gene expressions of proinflammatory cytokines and pattern recognition receptors (PRRs) in IPEC-J2 cells. Moreover, <i>L. plantarum</i> Lac16 preincubation effectively inhibited the phosphorylation of p65 caused by <i>C. perfringens</i> infection. Collectively, probiotic <i>L. plantarum</i> Lac16 exerts protective effects against <i>C. perfringens</i> infection-associated injury in IPEC-J2 cells.
format	article
author	Yuanhao Zhou Baikui Wang Qi Wang Li Tang Peng Zou Zihan Zeng Huihua Zhang Li Gong Weifen Li
author_facet	Yuanhao Zhou Baikui Wang Qi Wang Li Tang Peng Zou Zihan Zeng Huihua Zhang Li Gong Weifen Li
author_sort	Yuanhao Zhou
title	Protective Effects of <i>Lactobacillus plantarum</i> Lac16 on <i>Clostridium perfringens</i> Infection-Associated Injury in IPEC-J2 Cells
title_short	Protective Effects of <i>Lactobacillus plantarum</i> Lac16 on <i>Clostridium perfringens</i> Infection-Associated Injury in IPEC-J2 Cells
title_full	Protective Effects of <i>Lactobacillus plantarum</i> Lac16 on <i>Clostridium perfringens</i> Infection-Associated Injury in IPEC-J2 Cells
title_fullStr	Protective Effects of <i>Lactobacillus plantarum</i> Lac16 on <i>Clostridium perfringens</i> Infection-Associated Injury in IPEC-J2 Cells
title_full_unstemmed	Protective Effects of <i>Lactobacillus plantarum</i> Lac16 on <i>Clostridium perfringens</i> Infection-Associated Injury in IPEC-J2 Cells
title_sort	protective effects of <i>lactobacillus plantarum</i> lac16 on <i>clostridium perfringens</i> infection-associated injury in ipec-j2 cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/92448e1e2191427fa8d09d2539f7d829
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Protective Effects of <i>Lactobacillus plantarum</i> Lac16 on <i>Clostridium perfringens</i> Infection-Associated Injury in IPEC-J2 Cells

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