Differential oxidative stress induced by dengue virus in monocytes from human neonates, adult and elderly individuals.

Changes in immune response during lifespan of man are well known. These changes involve decreased neonatal and elderly immune response. In addition, it has been shown a relationship between immune and oxidative mechanisms, suggesting that altered immune response could be associated to altered oxidat...

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Autores principales:	Nereida Valero, Jesús Mosquera, Germán Añez, Alegria Levy, Rafael Marcucci, Melchor Alvarez de Mon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/926241627ab340a3b6e00c2bcee7bea2
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spelling	oai:doaj.org-article:926241627ab340a3b6e00c2bcee7bea22021-11-18T08:54:58ZDifferential oxidative stress induced by dengue virus in monocytes from human neonates, adult and elderly individuals.1932-620310.1371/journal.pone.0073221https://doaj.org/article/926241627ab340a3b6e00c2bcee7bea22013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24069178/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Changes in immune response during lifespan of man are well known. These changes involve decreased neonatal and elderly immune response. In addition, it has been shown a relationship between immune and oxidative mechanisms, suggesting that altered immune response could be associated to altered oxidative response. Increased expression of nitric oxide (NO) has been documented in dengue and in monocyte cultures infected with different types of dengue virus. However, there is no information about the age-dependent NO oxidative response in humans infected by dengue virus. In this study, monocyte cultures from neonatal, elderly and adult individuals (n = 10 each group) were infected with different dengue virus types (DENV- 1 to 4) and oxidative/antioxidative responses and apoptosis were measured at days 1 and 3 of culture. Increased production of NO, lipid peroxidation and enzymatic and nonenzymatic anti-oxidative responses in dengue infected monocyte cultures were observed. However, neonatal and elderly monocytes had lower values of studied parameters when compared to those in adult-derived cultures. Apoptosis was present in infected monocytes with higher values at day 3 of culture. This reduced oxidant/antioxidant response of neonatal and elderly monocytes could be relevant in the pathogenesis of dengue disease.Nereida ValeroJesús MosqueraGermán AñezAlegria LevyRafael MarcucciMelchor Alvarez de MonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73221 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nereida Valero Jesús Mosquera Germán Añez Alegria Levy Rafael Marcucci Melchor Alvarez de Mon Differential oxidative stress induced by dengue virus in monocytes from human neonates, adult and elderly individuals.
description	Changes in immune response during lifespan of man are well known. These changes involve decreased neonatal and elderly immune response. In addition, it has been shown a relationship between immune and oxidative mechanisms, suggesting that altered immune response could be associated to altered oxidative response. Increased expression of nitric oxide (NO) has been documented in dengue and in monocyte cultures infected with different types of dengue virus. However, there is no information about the age-dependent NO oxidative response in humans infected by dengue virus. In this study, monocyte cultures from neonatal, elderly and adult individuals (n = 10 each group) were infected with different dengue virus types (DENV- 1 to 4) and oxidative/antioxidative responses and apoptosis were measured at days 1 and 3 of culture. Increased production of NO, lipid peroxidation and enzymatic and nonenzymatic anti-oxidative responses in dengue infected monocyte cultures were observed. However, neonatal and elderly monocytes had lower values of studied parameters when compared to those in adult-derived cultures. Apoptosis was present in infected monocytes with higher values at day 3 of culture. This reduced oxidant/antioxidant response of neonatal and elderly monocytes could be relevant in the pathogenesis of dengue disease.
format	article
author	Nereida Valero Jesús Mosquera Germán Añez Alegria Levy Rafael Marcucci Melchor Alvarez de Mon
author_facet	Nereida Valero Jesús Mosquera Germán Añez Alegria Levy Rafael Marcucci Melchor Alvarez de Mon
author_sort	Nereida Valero
title	Differential oxidative stress induced by dengue virus in monocytes from human neonates, adult and elderly individuals.
title_short	Differential oxidative stress induced by dengue virus in monocytes from human neonates, adult and elderly individuals.
title_full	Differential oxidative stress induced by dengue virus in monocytes from human neonates, adult and elderly individuals.
title_fullStr	Differential oxidative stress induced by dengue virus in monocytes from human neonates, adult and elderly individuals.
title_full_unstemmed	Differential oxidative stress induced by dengue virus in monocytes from human neonates, adult and elderly individuals.
title_sort	differential oxidative stress induced by dengue virus in monocytes from human neonates, adult and elderly individuals.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/926241627ab340a3b6e00c2bcee7bea2
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Differential oxidative stress induced by dengue virus in monocytes from human neonates, adult and elderly individuals.

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