Doxorubicin-loaded cell-derived nanovesicles: an alternative targeted approach for anti-tumor therapy

Wei Jiang Goh,1,2 Choon Keong Lee,2 Shui Zou,2 Esther CY Woon,2 Bertrand Czarny,2,3 Giorgia Pastorin1,2,4 1NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), 2Department of Pharmacy, National University of Singapore, 3School of Materials Science and Engin...

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Autores principales:	Goh WJ, Lee CK, Zou S, Woon EC, Czarny B, Pastorin G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Cell Derived Nanovesicles cell targeting doxorubicin anti-tumour therapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9263617609e54b8ba555456b050348f7
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spelling	oai:doaj.org-article:9263617609e54b8ba555456b050348f72021-12-02T00:35:53ZDoxorubicin-loaded cell-derived nanovesicles: an alternative targeted approach for anti-tumor therapy1178-2013https://doaj.org/article/9263617609e54b8ba555456b050348f72017-04-01T00:00:00Zhttps://www.dovepress.com/doxorubicin-loaded-cell-derived-nanovesicles-an-alternative-targeted-a-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wei Jiang Goh,1,2 Choon Keong Lee,2 Shui Zou,2 Esther CY Woon,2 Bertrand Czarny,2,3 Giorgia Pastorin1,2,4 1NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), 2Department of Pharmacy, National University of Singapore, 3School of Materials Science and Engineering (MSE) & Lee Kong Chian School of Medicine, Nanyang Technological University, 4NUSNNI-NanoCore, National University of Singapore, T-Lab, Singapore, Singapore Abstract: Cell-derived nanovesicles (CDNs) are an emerging class of biological drug delivery systems (DDS) that retain the characteristics of the cells they were derived from, without the need for further surface functionalization. CDNs are also biocompatible, being derived from natural sources and also take advantage of the enhanced permeability and retention effect due to their nanodimensions. Furthermore, CDNs derived from monocytes were shown to have an in vivo targeting effect, accumulating at the tumor site in a previous study conducted in a mouse tumor model. Here, we report a systematic approach pertaining to various loading methods of the chemotherapeutic drug doxorubicin into our CDNs and examine the differential cellular uptake of drug-loaded CDNs in cancerous (HeLa) and healthy (HEK293) cell lines. Lastly, we proved that the addition of doxorubicin-loaded CDNs to the HeLa and HEK293 co-cultures showed a clear discrimination toward cancer cells at the cellular level. Our results further reinforce the intriguing potential of CDNs as an alternative targeted strategy for anticancer therapy. Keywords: cell-derived nanovesicles, cell targeting, doxorubicin, antitumor therapy, extracellular vesicles, biomimetic, bionanotechnology, antitumor strategiesGoh WJLee CKZou SWoon ECCzarny BPastorin GDove Medical PressarticleCell Derived Nanovesiclescell targetingdoxorubicinanti-tumour therapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2759-2767 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Derived Nanovesicles cell targeting doxorubicin anti-tumour therapy Medicine (General) R5-920
spellingShingle	Cell Derived Nanovesicles cell targeting doxorubicin anti-tumour therapy Medicine (General) R5-920 Goh WJ Lee CK Zou S Woon EC Czarny B Pastorin G Doxorubicin-loaded cell-derived nanovesicles: an alternative targeted approach for anti-tumor therapy
description	Wei Jiang Goh,1,2 Choon Keong Lee,2 Shui Zou,2 Esther CY Woon,2 Bertrand Czarny,2,3 Giorgia Pastorin1,2,4 1NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), 2Department of Pharmacy, National University of Singapore, 3School of Materials Science and Engineering (MSE) & Lee Kong Chian School of Medicine, Nanyang Technological University, 4NUSNNI-NanoCore, National University of Singapore, T-Lab, Singapore, Singapore Abstract: Cell-derived nanovesicles (CDNs) are an emerging class of biological drug delivery systems (DDS) that retain the characteristics of the cells they were derived from, without the need for further surface functionalization. CDNs are also biocompatible, being derived from natural sources and also take advantage of the enhanced permeability and retention effect due to their nanodimensions. Furthermore, CDNs derived from monocytes were shown to have an in vivo targeting effect, accumulating at the tumor site in a previous study conducted in a mouse tumor model. Here, we report a systematic approach pertaining to various loading methods of the chemotherapeutic drug doxorubicin into our CDNs and examine the differential cellular uptake of drug-loaded CDNs in cancerous (HeLa) and healthy (HEK293) cell lines. Lastly, we proved that the addition of doxorubicin-loaded CDNs to the HeLa and HEK293 co-cultures showed a clear discrimination toward cancer cells at the cellular level. Our results further reinforce the intriguing potential of CDNs as an alternative targeted strategy for anticancer therapy. Keywords: cell-derived nanovesicles, cell targeting, doxorubicin, antitumor therapy, extracellular vesicles, biomimetic, bionanotechnology, antitumor strategies
format	article
author	Goh WJ Lee CK Zou S Woon EC Czarny B Pastorin G
author_facet	Goh WJ Lee CK Zou S Woon EC Czarny B Pastorin G
author_sort	Goh WJ
title	Doxorubicin-loaded cell-derived nanovesicles: an alternative targeted approach for anti-tumor therapy
title_short	Doxorubicin-loaded cell-derived nanovesicles: an alternative targeted approach for anti-tumor therapy
title_full	Doxorubicin-loaded cell-derived nanovesicles: an alternative targeted approach for anti-tumor therapy
title_fullStr	Doxorubicin-loaded cell-derived nanovesicles: an alternative targeted approach for anti-tumor therapy
title_full_unstemmed	Doxorubicin-loaded cell-derived nanovesicles: an alternative targeted approach for anti-tumor therapy
title_sort	doxorubicin-loaded cell-derived nanovesicles: an alternative targeted approach for anti-tumor therapy
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/9263617609e54b8ba555456b050348f7
work_keys_str_mv	AT gohwj doxorubicinloadedcellderivednanovesiclesanalternativetargetedapproachforantitumortherapy AT leeck doxorubicinloadedcellderivednanovesiclesanalternativetargetedapproachforantitumortherapy AT zous doxorubicinloadedcellderivednanovesiclesanalternativetargetedapproachforantitumortherapy AT woonec doxorubicinloadedcellderivednanovesiclesanalternativetargetedapproachforantitumortherapy AT czarnyb doxorubicinloadedcellderivednanovesiclesanalternativetargetedapproachforantitumortherapy AT pastoring doxorubicinloadedcellderivednanovesiclesanalternativetargetedapproachforantitumortherapy
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Doxorubicin-loaded cell-derived nanovesicles: an alternative targeted approach for anti-tumor therapy

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