Aberrant plasticity of peripheral sensory axons in a painful neuropathy

Abstract Neuronal cells express considerable plasticity responding to environmental cues, in part, through subcellular mRNA regulation. Here we report on the extensive changes in distribution of mRNAs in the cell body and axon compartments of peripheral sensory neurons and the 3′ untranslated region...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Takashi Hirai, Yatendra Mulpuri, Yanbing Cheng, Zheng Xia, Wei Li, Supanigar Ruangsri, Igor Spigelman, Ichiro Nishimura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/92648845e8ef4cd8b775e4de3fde312b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:92648845e8ef4cd8b775e4de3fde312b
record_format	dspace
spelling	oai:doaj.org-article:92648845e8ef4cd8b775e4de3fde312b2021-12-02T12:30:46ZAberrant plasticity of peripheral sensory axons in a painful neuropathy10.1038/s41598-017-03390-92045-2322https://doaj.org/article/92648845e8ef4cd8b775e4de3fde312b2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03390-9https://doaj.org/toc/2045-2322Abstract Neuronal cells express considerable plasticity responding to environmental cues, in part, through subcellular mRNA regulation. Here we report on the extensive changes in distribution of mRNAs in the cell body and axon compartments of peripheral sensory neurons and the 3′ untranslated region (3′UTR) landscapes after unilateral sciatic nerve entrapment (SNE) injury in rats. Neuronal cells dissociated from SNE-injured and contralateral L4 and L5 dorsal root ganglia were cultured in a compartmentalized system. Axonal and cell body RNA samples were separately subjected to high throughput RNA sequencing (RNA-Seq). The injured axons exhibited enrichment of mRNAs related to protein synthesis and nerve regeneration. Lengthening of 3′UTRs was more prevalent in the injured axons, including the newly discovered alternative cleavage and polyadenylation of NaV1.8 mRNA. Alternative polyadenylation was largely independent from the relative abundance of axonal mRNAs; but they were highly clustered in functional pathways related to RNA granule formation in the injured axons. These RNA-Seq data analyses indicate that peripheral nerve injury may result in highly selective mRNA enrichment in the affected axons with 3′UTR alterations potentially contributing to the mechanism of neuropathic pain.Takashi HiraiYatendra MulpuriYanbing ChengZheng XiaWei LiSupanigar RuangsriIgor SpigelmanIchiro NishimuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takashi Hirai Yatendra Mulpuri Yanbing Cheng Zheng Xia Wei Li Supanigar Ruangsri Igor Spigelman Ichiro Nishimura Aberrant plasticity of peripheral sensory axons in a painful neuropathy
description	Abstract Neuronal cells express considerable plasticity responding to environmental cues, in part, through subcellular mRNA regulation. Here we report on the extensive changes in distribution of mRNAs in the cell body and axon compartments of peripheral sensory neurons and the 3′ untranslated region (3′UTR) landscapes after unilateral sciatic nerve entrapment (SNE) injury in rats. Neuronal cells dissociated from SNE-injured and contralateral L4 and L5 dorsal root ganglia were cultured in a compartmentalized system. Axonal and cell body RNA samples were separately subjected to high throughput RNA sequencing (RNA-Seq). The injured axons exhibited enrichment of mRNAs related to protein synthesis and nerve regeneration. Lengthening of 3′UTRs was more prevalent in the injured axons, including the newly discovered alternative cleavage and polyadenylation of NaV1.8 mRNA. Alternative polyadenylation was largely independent from the relative abundance of axonal mRNAs; but they were highly clustered in functional pathways related to RNA granule formation in the injured axons. These RNA-Seq data analyses indicate that peripheral nerve injury may result in highly selective mRNA enrichment in the affected axons with 3′UTR alterations potentially contributing to the mechanism of neuropathic pain.
format	article
author	Takashi Hirai Yatendra Mulpuri Yanbing Cheng Zheng Xia Wei Li Supanigar Ruangsri Igor Spigelman Ichiro Nishimura
author_facet	Takashi Hirai Yatendra Mulpuri Yanbing Cheng Zheng Xia Wei Li Supanigar Ruangsri Igor Spigelman Ichiro Nishimura
author_sort	Takashi Hirai
title	Aberrant plasticity of peripheral sensory axons in a painful neuropathy
title_short	Aberrant plasticity of peripheral sensory axons in a painful neuropathy
title_full	Aberrant plasticity of peripheral sensory axons in a painful neuropathy
title_fullStr	Aberrant plasticity of peripheral sensory axons in a painful neuropathy
title_full_unstemmed	Aberrant plasticity of peripheral sensory axons in a painful neuropathy
title_sort	aberrant plasticity of peripheral sensory axons in a painful neuropathy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/92648845e8ef4cd8b775e4de3fde312b
work_keys_str_mv	AT takashihirai aberrantplasticityofperipheralsensoryaxonsinapainfulneuropathy AT yatendramulpuri aberrantplasticityofperipheralsensoryaxonsinapainfulneuropathy AT yanbingcheng aberrantplasticityofperipheralsensoryaxonsinapainfulneuropathy AT zhengxia aberrantplasticityofperipheralsensoryaxonsinapainfulneuropathy AT weili aberrantplasticityofperipheralsensoryaxonsinapainfulneuropathy AT supanigarruangsri aberrantplasticityofperipheralsensoryaxonsinapainfulneuropathy AT igorspigelman aberrantplasticityofperipheralsensoryaxonsinapainfulneuropathy AT ichironishimura aberrantplasticityofperipheralsensoryaxonsinapainfulneuropathy
_version_	1718394347938906112

Aberrant plasticity of peripheral sensory axons in a painful neuropathy

Ejemplares similares