Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human

Abstract Human Corneal epithelial stem cells (CESCs) have been identified to reside in limbus for more than 2 decades. However, the precise location of CESCs in other mammalian remains elusive. This study was to identify differential localization of putative CESCs in mice. Through a series of murine...

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Autores principales:	Jin Li, Yangyan Xiao, Terry G. Coursey, Xin Chen, Ruzhi Deng, Fan Lu, Stephen C. Pflugfelder, De-Quan Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/926de7578d944ef5b0b44a4da7f008e9
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spelling	oai:doaj.org-article:926de7578d944ef5b0b44a4da7f008e92021-12-02T15:06:24ZIdentification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human10.1038/s41598-017-04569-w2045-2322https://doaj.org/article/926de7578d944ef5b0b44a4da7f008e92017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04569-whttps://doaj.org/toc/2045-2322Abstract Human Corneal epithelial stem cells (CESCs) have been identified to reside in limbus for more than 2 decades. However, the precise location of CESCs in other mammalian remains elusive. This study was to identify differential localization of putative CESCs in mice. Through a series of murine corneal cross-sections from different directions, we identified that anatomically and morphologically the murine limbus is composed of the thinnest epithelium and the thinnest stroma without any palisades of Vogt-like niche structure. The cells expressing five of stem/progenitor cell markers are localized in basal layer of entire murine corneal epithelium. BrdU label-retaining cells, a key characteristic of epithelial stem cells, are detected in both limbal and central cornea of mouse eye. Functionally, corneal epithelium can be regenerated in cultures from central and limbal explants of murine cornea. Such a distribution of mouse CESCs is different from human cornea, where limbal stem cell concept has been well established and accepted. We are aware that some new evidence supports limbal stem cell concept in mouse recently. However, it is important to know that central cornea may provide an alternative source of stem cells when one utilizes mice as animal model for corneal research.Jin LiYangyan XiaoTerry G. CourseyXin ChenRuzhi DengFan LuStephen C. PflugfelderDe-Quan LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jin Li Yangyan Xiao Terry G. Coursey Xin Chen Ruzhi Deng Fan Lu Stephen C. Pflugfelder De-Quan Li Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human
description	Abstract Human Corneal epithelial stem cells (CESCs) have been identified to reside in limbus for more than 2 decades. However, the precise location of CESCs in other mammalian remains elusive. This study was to identify differential localization of putative CESCs in mice. Through a series of murine corneal cross-sections from different directions, we identified that anatomically and morphologically the murine limbus is composed of the thinnest epithelium and the thinnest stroma without any palisades of Vogt-like niche structure. The cells expressing five of stem/progenitor cell markers are localized in basal layer of entire murine corneal epithelium. BrdU label-retaining cells, a key characteristic of epithelial stem cells, are detected in both limbal and central cornea of mouse eye. Functionally, corneal epithelium can be regenerated in cultures from central and limbal explants of murine cornea. Such a distribution of mouse CESCs is different from human cornea, where limbal stem cell concept has been well established and accepted. We are aware that some new evidence supports limbal stem cell concept in mouse recently. However, it is important to know that central cornea may provide an alternative source of stem cells when one utilizes mice as animal model for corneal research.
format	article
author	Jin Li Yangyan Xiao Terry G. Coursey Xin Chen Ruzhi Deng Fan Lu Stephen C. Pflugfelder De-Quan Li
author_facet	Jin Li Yangyan Xiao Terry G. Coursey Xin Chen Ruzhi Deng Fan Lu Stephen C. Pflugfelder De-Quan Li
author_sort	Jin Li
title	Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human
title_short	Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human
title_full	Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human
title_fullStr	Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human
title_full_unstemmed	Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human
title_sort	identification for differential localization of putative corneal epithelial stem cells in mouse and human
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/926de7578d944ef5b0b44a4da7f008e9
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_version_	1718388463426863104

Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human

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