Impact of minocycline on vascularization and visual function in an immature mouse model of ischemic retinopathy

Abstract The role of microglia in the pathophysiology of ischemic retinal diseases has been extensively studied. Retinal microglial activation may be correlated with retinal neovascularization in oxygen-induced retinopathy (OIR), an animal model that has been widely used in retinopathy of prematurit...

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Autores principales:	Wenqin Xu, Jie Yin, Lijuan Sun, Zhicha Hu, Guorui Dou, Zifeng Zhang, Haiyan Wang, Changmei Guo, Yusheng Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/92716593bbc5490fb7fd63d0262e65a3
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spelling	oai:doaj.org-article:92716593bbc5490fb7fd63d0262e65a32021-12-02T15:05:14ZImpact of minocycline on vascularization and visual function in an immature mouse model of ischemic retinopathy10.1038/s41598-017-07978-z2045-2322https://doaj.org/article/92716593bbc5490fb7fd63d0262e65a32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07978-zhttps://doaj.org/toc/2045-2322Abstract The role of microglia in the pathophysiology of ischemic retinal diseases has been extensively studied. Retinal microglial activation may be correlated with retinal neovascularization in oxygen-induced retinopathy (OIR), an animal model that has been widely used in retinopathy of prematurity (ROP) research. Minocycline is an antibiotic that decreases microglial activation following hyperoxic and hypoxic-ischemic phases in neonatal rodents. Here, we investigated the effects of minocycline on vascularization and visual function. In our results, we found that after the administration of minocycline, microglial reactivity was reduced in the retina, which was accompanied by an increase in the avascular area at P12, P14 and P17. Although microglial reactivity was reduced at P17, minocycline treatment did not attenuate retinal neovascularization. A changing trend in microglial number was observed, and the apoptosis and proliferation states on different days partly contributed to this change. Further study also revealed that although minocycline downregulated the levels of proinflammatory factors, visual function appeared to be significantly worsened. Collectively, we demonstrated that minocycline disturbed the physiological vascularization of the avascular area and exacerbated visual dysfunction, indicating that minocycline may not be an effective drug and may even be detrimental for the treatment of ischemic retinopathy in immature mammals.Wenqin XuJie YinLijuan SunZhicha HuGuorui DouZifeng ZhangHaiyan WangChangmei GuoYusheng WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenqin Xu Jie Yin Lijuan Sun Zhicha Hu Guorui Dou Zifeng Zhang Haiyan Wang Changmei Guo Yusheng Wang Impact of minocycline on vascularization and visual function in an immature mouse model of ischemic retinopathy
description	Abstract The role of microglia in the pathophysiology of ischemic retinal diseases has been extensively studied. Retinal microglial activation may be correlated with retinal neovascularization in oxygen-induced retinopathy (OIR), an animal model that has been widely used in retinopathy of prematurity (ROP) research. Minocycline is an antibiotic that decreases microglial activation following hyperoxic and hypoxic-ischemic phases in neonatal rodents. Here, we investigated the effects of minocycline on vascularization and visual function. In our results, we found that after the administration of minocycline, microglial reactivity was reduced in the retina, which was accompanied by an increase in the avascular area at P12, P14 and P17. Although microglial reactivity was reduced at P17, minocycline treatment did not attenuate retinal neovascularization. A changing trend in microglial number was observed, and the apoptosis and proliferation states on different days partly contributed to this change. Further study also revealed that although minocycline downregulated the levels of proinflammatory factors, visual function appeared to be significantly worsened. Collectively, we demonstrated that minocycline disturbed the physiological vascularization of the avascular area and exacerbated visual dysfunction, indicating that minocycline may not be an effective drug and may even be detrimental for the treatment of ischemic retinopathy in immature mammals.
format	article
author	Wenqin Xu Jie Yin Lijuan Sun Zhicha Hu Guorui Dou Zifeng Zhang Haiyan Wang Changmei Guo Yusheng Wang
author_facet	Wenqin Xu Jie Yin Lijuan Sun Zhicha Hu Guorui Dou Zifeng Zhang Haiyan Wang Changmei Guo Yusheng Wang
author_sort	Wenqin Xu
title	Impact of minocycline on vascularization and visual function in an immature mouse model of ischemic retinopathy
title_short	Impact of minocycline on vascularization and visual function in an immature mouse model of ischemic retinopathy
title_full	Impact of minocycline on vascularization and visual function in an immature mouse model of ischemic retinopathy
title_fullStr	Impact of minocycline on vascularization and visual function in an immature mouse model of ischemic retinopathy
title_full_unstemmed	Impact of minocycline on vascularization and visual function in an immature mouse model of ischemic retinopathy
title_sort	impact of minocycline on vascularization and visual function in an immature mouse model of ischemic retinopathy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/92716593bbc5490fb7fd63d0262e65a3
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_version_	1718388906126213120

Impact of minocycline on vascularization and visual function in an immature mouse model of ischemic retinopathy

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