Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22

Low molecular weight (LMW) antiplasmodial compounds isolated from bacteria, particularly Streptomyces have not been widely reported. This study aimed to identify LMW compounds from Streptomyces sp. GMR22 as antiplasmodial. Isolation of the LMW compounds from the supernatant of fermentation culture u...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tarsa Ruli Tambunan, Jaka Widada, Ema Damayanti, Tutik Dwi Wahyuningsih, Mustofa Mustofa
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2020
Materias:	low molecular weight compounds streptomyces antiplasmodial antimalarial Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/9278a76e578c4773bc075a01320d5fe7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9278a76e578c4773bc075a01320d5fe7
record_format	dspace
spelling	oai:doaj.org-article:9278a76e578c4773bc075a01320d5fe72021-11-15T06:08:47ZAntiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR222338-94272338-948610.22146/ijp.634https://doaj.org/article/9278a76e578c4773bc075a01320d5fe72020-12-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/634https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Low molecular weight (LMW) antiplasmodial compounds isolated from bacteria, particularly Streptomyces have not been widely reported. This study aimed to identify LMW compounds from Streptomyces sp. GMR22 as antiplasmodial. Isolation of the LMW compounds from the supernatant of fermentation culture using solvent of n-hexane:ethylacetate (EtOAc) (85:15v/v)and identified using gas chromatography-mass spectrometry (GC-MS). Antiplasmodial assay of n-hexane:EtOAc extract was carried out in vitro against P. falciparum (3D7). Parasitemia percentage obtained through microscopic observations and 50% inhibitory concentration (IC50) obtained through probit analysis. The antiplasmodial confirmation test was done by flow cytometry using SYBR Green I for Plasmodium DNA and anti-human CD235a for erythrocytes. The LMW compounds were investigated using SwissADME for drug-likeness. n-Hexane:EtOAc extract contained 21 LMW compounds from alcohol, hydrocarbon, ester, aromatic/diester, diester, fatty acid, and triester classes, which possessed moderate antiplasmodial activity with an IC50 value of 38.61 ± 19.06 µg/mL. Confirmation by flow cytometry analysis showed that the extract at 50 µg/mL exhibited antiplasmodial activity based on a decreased Plasmodium DNA intensity as compared to the control group. The result of drug-likeness screening obtained that 3 LMW compounds were drug-likeness, namely phenylethyl alcohol, ethyl citrate, and di-n-butyl phthalate. Streptomyces sp. GMR22 produced LMW compounds as antiplasmodial, and further study was needed for identification of antiplasmodial active compounds.Tarsa Ruli TambunanJaka WidadaEma DamayantiTutik Dwi WahyuningsihMustofa MustofaUniversitas Gadjah Madaarticlelow molecular weight compoundsstreptomycesantiplasmodialantimalarialPharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 273-280 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	low molecular weight compounds streptomyces antiplasmodial antimalarial Pharmacy and materia medica RS1-441
spellingShingle	low molecular weight compounds streptomyces antiplasmodial antimalarial Pharmacy and materia medica RS1-441 Tarsa Ruli Tambunan Jaka Widada Ema Damayanti Tutik Dwi Wahyuningsih Mustofa Mustofa Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22
description	Low molecular weight (LMW) antiplasmodial compounds isolated from bacteria, particularly Streptomyces have not been widely reported. This study aimed to identify LMW compounds from Streptomyces sp. GMR22 as antiplasmodial. Isolation of the LMW compounds from the supernatant of fermentation culture using solvent of n-hexane:ethylacetate (EtOAc) (85:15v/v)and identified using gas chromatography-mass spectrometry (GC-MS). Antiplasmodial assay of n-hexane:EtOAc extract was carried out in vitro against P. falciparum (3D7). Parasitemia percentage obtained through microscopic observations and 50% inhibitory concentration (IC50) obtained through probit analysis. The antiplasmodial confirmation test was done by flow cytometry using SYBR Green I for Plasmodium DNA and anti-human CD235a for erythrocytes. The LMW compounds were investigated using SwissADME for drug-likeness. n-Hexane:EtOAc extract contained 21 LMW compounds from alcohol, hydrocarbon, ester, aromatic/diester, diester, fatty acid, and triester classes, which possessed moderate antiplasmodial activity with an IC50 value of 38.61 ± 19.06 µg/mL. Confirmation by flow cytometry analysis showed that the extract at 50 µg/mL exhibited antiplasmodial activity based on a decreased Plasmodium DNA intensity as compared to the control group. The result of drug-likeness screening obtained that 3 LMW compounds were drug-likeness, namely phenylethyl alcohol, ethyl citrate, and di-n-butyl phthalate. Streptomyces sp. GMR22 produced LMW compounds as antiplasmodial, and further study was needed for identification of antiplasmodial active compounds.
format	article
author	Tarsa Ruli Tambunan Jaka Widada Ema Damayanti Tutik Dwi Wahyuningsih Mustofa Mustofa
author_facet	Tarsa Ruli Tambunan Jaka Widada Ema Damayanti Tutik Dwi Wahyuningsih Mustofa Mustofa
author_sort	Tarsa Ruli Tambunan
title	Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22
title_short	Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22
title_full	Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22
title_fullStr	Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22
title_full_unstemmed	Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22
title_sort	antiplasmodial activity of the low molecular weight compounds from streptomyces sp. gmr22
publisher	Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/9278a76e578c4773bc075a01320d5fe7
work_keys_str_mv	AT tarsarulitambunan antiplasmodialactivityofthelowmolecularweightcompoundsfromstreptomycesspgmr22 AT jakawidada antiplasmodialactivityofthelowmolecularweightcompoundsfromstreptomycesspgmr22 AT emadamayanti antiplasmodialactivityofthelowmolecularweightcompoundsfromstreptomycesspgmr22 AT tutikdwiwahyuningsih antiplasmodialactivityofthelowmolecularweightcompoundsfromstreptomycesspgmr22 AT mustofamustofa antiplasmodialactivityofthelowmolecularweightcompoundsfromstreptomycesspgmr22
_version_	1718428566910140416

Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22

Ejemplares similares