Development and validation of HPLC-MS2 methodology for the accurate determination of C4–C8 B-type flavanols and procyanidins

Abstract Cocoa flavanols and procyanidins (CFs), natural dietary bioactives, have been studied extensively over the past two decades for their potential health benefits. Research on their safety and efficacy is critically dependent upon on the ability to reliably characeterize the research materials...

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Autores principales:	Ugo Bussy, Yusuf Olanrewaju, Alan Crozier, Javier Ottaviani, Catherine Kwik-Uribe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/927eb22b06ed498faf035d5781e6ffa4
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spelling	oai:doaj.org-article:927eb22b06ed498faf035d5781e6ffa42021-12-02T16:17:27ZDevelopment and validation of HPLC-MS2 methodology for the accurate determination of C4–C8 B-type flavanols and procyanidins10.1038/s41598-021-93993-02045-2322https://doaj.org/article/927eb22b06ed498faf035d5781e6ffa42021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93993-0https://doaj.org/toc/2045-2322Abstract Cocoa flavanols and procyanidins (CFs), natural dietary bioactives, have been studied extensively over the past two decades for their potential health benefits. Research on their safety and efficacy is critically dependent upon on the ability to reliably characeterize the research materials that are utilized, and with growing consumer availability of CF-based products, reliable methods for the detection of potential adulteration are of increasing importance. This research focused on the development of a high performance liquid chromatography-tandem mass spectrometry method (HPLC-MS2) using primary standards and 13C-labelled procyanidins as internal standards. The ability of MS2 detection to discriminate A- and B-type procyanidins was demonstrated. Method performances were validated for degrees of polymerization up to four in seven model food matrices. Accuracy ranged from 90.9 to 125.4% and precision was < 10% at lower concentrations. Finally, the method was applied to cocoa-based samples and compared to the AOAC 2020.05 analytical protocol, supporting the use of NIST 8403 as reference material for HPLC-MS2 analysis.Ugo BussyYusuf OlanrewajuAlan CrozierJavier OttavianiCatherine Kwik-UribeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ugo Bussy Yusuf Olanrewaju Alan Crozier Javier Ottaviani Catherine Kwik-Uribe Development and validation of HPLC-MS2 methodology for the accurate determination of C4–C8 B-type flavanols and procyanidins
description	Abstract Cocoa flavanols and procyanidins (CFs), natural dietary bioactives, have been studied extensively over the past two decades for their potential health benefits. Research on their safety and efficacy is critically dependent upon on the ability to reliably characeterize the research materials that are utilized, and with growing consumer availability of CF-based products, reliable methods for the detection of potential adulteration are of increasing importance. This research focused on the development of a high performance liquid chromatography-tandem mass spectrometry method (HPLC-MS2) using primary standards and 13C-labelled procyanidins as internal standards. The ability of MS2 detection to discriminate A- and B-type procyanidins was demonstrated. Method performances were validated for degrees of polymerization up to four in seven model food matrices. Accuracy ranged from 90.9 to 125.4% and precision was < 10% at lower concentrations. Finally, the method was applied to cocoa-based samples and compared to the AOAC 2020.05 analytical protocol, supporting the use of NIST 8403 as reference material for HPLC-MS2 analysis.
format	article
author	Ugo Bussy Yusuf Olanrewaju Alan Crozier Javier Ottaviani Catherine Kwik-Uribe
author_facet	Ugo Bussy Yusuf Olanrewaju Alan Crozier Javier Ottaviani Catherine Kwik-Uribe
author_sort	Ugo Bussy
title	Development and validation of HPLC-MS2 methodology for the accurate determination of C4–C8 B-type flavanols and procyanidins
title_short	Development and validation of HPLC-MS2 methodology for the accurate determination of C4–C8 B-type flavanols and procyanidins
title_full	Development and validation of HPLC-MS2 methodology for the accurate determination of C4–C8 B-type flavanols and procyanidins
title_fullStr	Development and validation of HPLC-MS2 methodology for the accurate determination of C4–C8 B-type flavanols and procyanidins
title_full_unstemmed	Development and validation of HPLC-MS2 methodology for the accurate determination of C4–C8 B-type flavanols and procyanidins
title_sort	development and validation of hplc-ms2 methodology for the accurate determination of c4–c8 b-type flavanols and procyanidins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/927eb22b06ed498faf035d5781e6ffa4
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Development and validation of HPLC-MS2 methodology for the accurate determination of C4–C8 B-type flavanols and procyanidins

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