Genomic Analysis of Emerging Florfenicol-Resistant <named-content content-type="genus-species">Campylobacter coli</named-content> Isolated from the Cecal Contents of Cattle in the United States

ABSTRACT Genomic analyses were performed on florfenicol-resistant (FFNr) Campylobacter coli isolates recovered from cattle, and the cfr(C) gene-associated multidrug resistance (MDR) plasmid was characterized. Sixteen FFNr C. coli isolates recovered between 2013 and 2018 from beef cattle were sequenc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shaohua Zhao, Sampa Mukherjee, Chih-Hao Hsu, Shenia Young, Cong Li, Heather Tate, Cesar A. Morales, Jovita Haro, Sutawee Thitaram, Glenn E. Tillman, Uday Dessai, Patrick McDermott
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Campylobacter MDR NARMS WGS florfenicol resistance plasmid Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9286b61598bd42f1b16cfc1334fbbf6b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9286b61598bd42f1b16cfc1334fbbf6b
record_format	dspace
spelling	oai:doaj.org-article:9286b61598bd42f1b16cfc1334fbbf6b2021-11-15T15:22:20ZGenomic Analysis of Emerging Florfenicol-Resistant <named-content content-type="genus-species">Campylobacter coli</named-content> Isolated from the Cecal Contents of Cattle in the United States10.1128/mSphere.00367-192379-5042https://doaj.org/article/9286b61598bd42f1b16cfc1334fbbf6b2019-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00367-19https://doaj.org/toc/2379-5042ABSTRACT Genomic analyses were performed on florfenicol-resistant (FFNr) Campylobacter coli isolates recovered from cattle, and the cfr(C) gene-associated multidrug resistance (MDR) plasmid was characterized. Sixteen FFNr C. coli isolates recovered between 2013 and 2018 from beef cattle were sequenced using MiSeq. Genomes and plasmids were found to be closed for three of the isolates using the PacBio system. Single nucleotide polymorphisms (SNPs) across the genome and the structures of MDR plasmids were investigated. Conjugation experiments were performed to determine the transferability of cfr(C)-associated MDR plasmids. The spectrum of resistance encoded by the cfr(C) gene was further investigated by agar dilution antimicrobial susceptibility testing. All 16 FFNr isolates were MDR and exhibited coresistance to ciprofloxacin, nalidixic acid, clindamycin, and tetracycline. All isolates shared the same resistance genotype, carrying aph (3′)-III, hph, ΔaadE (truncated), blaOXA-61, cfr(C), and tet(O) genes plus a mutation of GyrA (T86I). The cfr(C), aph (3′)-III, hph, ΔaadE, and tet(O) genes were colocated on transferable MDR plasmids ranging in size from 48 to 50 kb. These plasmids showed high sequence homology with the pTet plasmid and carried several Campylobacter virulence genes, including virB2, virB4, virB5, VirB6, virB7, virB8, virb9, virB10, virB11, and virD4. The cfr(C) gene conferred resistance to florfenicol (8 to 32 μg/ml), clindamycin (512 to 1,024 μg/ml), linezolid (128 to 512 μg/ml), and tiamulin (1,024 μg/ml). Phylogenetic analysis showed SNP differences ranging from 11 to 2,248 SNPs among the 16 isolates. The results showed that the cfr(C) gene located in the conjugative pTet MDR/virulence plasmid is present in diverse strains, where it confers high levels of resistance to several antimicrobials, including linezolid, a critical drug for treating infections by Gram-positive bacteria in humans. This report highlights the power of genomic antimicrobial resistance surveillance to uncover the intricacies of transmissible coresistance and provides information that is needed for accurate risk assessment and mitigation strategies. IMPORTANCE Campylobacter is a leading cause of foodborne diarrheal illness worldwide, with more than one million cases each year in the United States alone. The global emergence of antimicrobial resistance in this pathogen has become a growing public health concern. Florfenicol-resistant (FFNr) Campylobacter has been very rare in the United States. In this study, we employed whole-genome sequencing to characterize 16 multidrug-resistant Campylobacter coli isolates recovered from cattle in the United States. A gene [cfr(C)] was found to be responsible for resistance not only to florfenicol but also to several other antimicrobials, including linezolid, a critical drug for treating infections by Gram-positive bacteria in humans. The results showed that cfr(C) is located in a conjugative pTet MDR/virulence plasmid. This report highlights the power of antimicrobial resistance surveillance to uncover the intricacies of transmissible coresistance and provides information that is needed for accurate risk assessment and mitigation strategies.Shaohua ZhaoSampa MukherjeeChih-Hao HsuShenia YoungCong LiHeather TateCesar A. MoralesJovita HaroSutawee ThitaramGlenn E. TillmanUday DessaiPatrick McDermottAmerican Society for MicrobiologyarticleCampylobacterMDRNARMSWGSflorfenicol resistanceplasmidMicrobiologyQR1-502ENmSphere, Vol 4, Iss 3 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Campylobacter MDR NARMS WGS florfenicol resistance plasmid Microbiology QR1-502
spellingShingle	Campylobacter MDR NARMS WGS florfenicol resistance plasmid Microbiology QR1-502 Shaohua Zhao Sampa Mukherjee Chih-Hao Hsu Shenia Young Cong Li Heather Tate Cesar A. Morales Jovita Haro Sutawee Thitaram Glenn E. Tillman Uday Dessai Patrick McDermott Genomic Analysis of Emerging Florfenicol-Resistant <named-content content-type="genus-species">Campylobacter coli</named-content> Isolated from the Cecal Contents of Cattle in the United States
description	ABSTRACT Genomic analyses were performed on florfenicol-resistant (FFNr) Campylobacter coli isolates recovered from cattle, and the cfr(C) gene-associated multidrug resistance (MDR) plasmid was characterized. Sixteen FFNr C. coli isolates recovered between 2013 and 2018 from beef cattle were sequenced using MiSeq. Genomes and plasmids were found to be closed for three of the isolates using the PacBio system. Single nucleotide polymorphisms (SNPs) across the genome and the structures of MDR plasmids were investigated. Conjugation experiments were performed to determine the transferability of cfr(C)-associated MDR plasmids. The spectrum of resistance encoded by the cfr(C) gene was further investigated by agar dilution antimicrobial susceptibility testing. All 16 FFNr isolates were MDR and exhibited coresistance to ciprofloxacin, nalidixic acid, clindamycin, and tetracycline. All isolates shared the same resistance genotype, carrying aph (3′)-III, hph, ΔaadE (truncated), blaOXA-61, cfr(C), and tet(O) genes plus a mutation of GyrA (T86I). The cfr(C), aph (3′)-III, hph, ΔaadE, and tet(O) genes were colocated on transferable MDR plasmids ranging in size from 48 to 50 kb. These plasmids showed high sequence homology with the pTet plasmid and carried several Campylobacter virulence genes, including virB2, virB4, virB5, VirB6, virB7, virB8, virb9, virB10, virB11, and virD4. The cfr(C) gene conferred resistance to florfenicol (8 to 32 μg/ml), clindamycin (512 to 1,024 μg/ml), linezolid (128 to 512 μg/ml), and tiamulin (1,024 μg/ml). Phylogenetic analysis showed SNP differences ranging from 11 to 2,248 SNPs among the 16 isolates. The results showed that the cfr(C) gene located in the conjugative pTet MDR/virulence plasmid is present in diverse strains, where it confers high levels of resistance to several antimicrobials, including linezolid, a critical drug for treating infections by Gram-positive bacteria in humans. This report highlights the power of genomic antimicrobial resistance surveillance to uncover the intricacies of transmissible coresistance and provides information that is needed for accurate risk assessment and mitigation strategies. IMPORTANCE Campylobacter is a leading cause of foodborne diarrheal illness worldwide, with more than one million cases each year in the United States alone. The global emergence of antimicrobial resistance in this pathogen has become a growing public health concern. Florfenicol-resistant (FFNr) Campylobacter has been very rare in the United States. In this study, we employed whole-genome sequencing to characterize 16 multidrug-resistant Campylobacter coli isolates recovered from cattle in the United States. A gene [cfr(C)] was found to be responsible for resistance not only to florfenicol but also to several other antimicrobials, including linezolid, a critical drug for treating infections by Gram-positive bacteria in humans. The results showed that cfr(C) is located in a conjugative pTet MDR/virulence plasmid. This report highlights the power of antimicrobial resistance surveillance to uncover the intricacies of transmissible coresistance and provides information that is needed for accurate risk assessment and mitigation strategies.
format	article
author	Shaohua Zhao Sampa Mukherjee Chih-Hao Hsu Shenia Young Cong Li Heather Tate Cesar A. Morales Jovita Haro Sutawee Thitaram Glenn E. Tillman Uday Dessai Patrick McDermott
author_facet	Shaohua Zhao Sampa Mukherjee Chih-Hao Hsu Shenia Young Cong Li Heather Tate Cesar A. Morales Jovita Haro Sutawee Thitaram Glenn E. Tillman Uday Dessai Patrick McDermott
author_sort	Shaohua Zhao
title	Genomic Analysis of Emerging Florfenicol-Resistant <named-content content-type="genus-species">Campylobacter coli</named-content> Isolated from the Cecal Contents of Cattle in the United States
title_short	Genomic Analysis of Emerging Florfenicol-Resistant <named-content content-type="genus-species">Campylobacter coli</named-content> Isolated from the Cecal Contents of Cattle in the United States
title_full	Genomic Analysis of Emerging Florfenicol-Resistant <named-content content-type="genus-species">Campylobacter coli</named-content> Isolated from the Cecal Contents of Cattle in the United States
title_fullStr	Genomic Analysis of Emerging Florfenicol-Resistant <named-content content-type="genus-species">Campylobacter coli</named-content> Isolated from the Cecal Contents of Cattle in the United States
title_full_unstemmed	Genomic Analysis of Emerging Florfenicol-Resistant <named-content content-type="genus-species">Campylobacter coli</named-content> Isolated from the Cecal Contents of Cattle in the United States
title_sort	genomic analysis of emerging florfenicol-resistant <named-content content-type="genus-species">campylobacter coli</named-content> isolated from the cecal contents of cattle in the united states
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/9286b61598bd42f1b16cfc1334fbbf6b
work_keys_str_mv	AT shaohuazhao genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT sampamukherjee genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT chihhaohsu genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT sheniayoung genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT congli genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT heathertate genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT cesaramorales genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT jovitaharo genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT sutaweethitaram genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT glennetillman genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT udaydessai genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates AT patrickmcdermott genomicanalysisofemergingflorfenicolresistantnamedcontentcontenttypegenusspeciescampylobactercolinamedcontentisolatedfromthececalcontentsofcattleintheunitedstates
_version_	1718428018631770112

Genomic Analysis of Emerging Florfenicol-Resistant <named-content content-type="genus-species">Campylobacter coli</named-content> Isolated from the Cecal Contents of Cattle in the United States

Ejemplares similares