The Potential Effect of Insulin on AChE and Its Interactions with Rivastigmine In Vitro

There is no definite cure for Alzheimer’s disease (AD) due to its multifactorial origin. Drugs that inhibit acetylcholinesterase (AChE), such as rivastigmine, are promising symptomatic treatments for AD. Emerging evidence suggests that insulin therapy can hinder several aspects of AD pathology. Insu...

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Autores principales:	Tahereh Jamshidnejad-Tosaramandani, Soheila Kashanian, Mahsa Babaei, Mohamed H. Al-Sabri, Helgi B. Schiöth
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Alzheimer disease insulin rivastigmine acetylcholinesterase inhibition combinational therapy Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/92921d97535b4d4ea6eb9d82c6c12859
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spelling	oai:doaj.org-article:92921d97535b4d4ea6eb9d82c6c128592021-11-25T18:39:38ZThe Potential Effect of Insulin on AChE and Its Interactions with Rivastigmine In Vitro10.3390/ph141111361424-8247https://doaj.org/article/92921d97535b4d4ea6eb9d82c6c128592021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1136https://doaj.org/toc/1424-8247There is no definite cure for Alzheimer’s disease (AD) due to its multifactorial origin. Drugs that inhibit acetylcholinesterase (AChE), such as rivastigmine, are promising symptomatic treatments for AD. Emerging evidence suggests that insulin therapy can hinder several aspects of AD pathology. Insulin has been shown to modify the activity of AChE, but it is still unknown how insulin and AChE interact. Combination therapy, which targets several features of the disease based on existing medications, can provide a worthy therapy option for AD management. However, to date, no studies have examined the potential interaction of insulin with AChE and/or rivastigmine in vitro. In the present study, we employed the Response Surface Methodology (RSM) as an in vitro assessment to investigate the effect of insulin on both AChE activity and rivastigmine inhibitory action using a common spectrophotometric assay for cholinesterase activity, Ellman’s method. Our results showed that insulin, even at high concentrations, has an insignificant effect on both the activity of AChE and rivastigmine’s inhibitory action. The variance of our data is near zero, which means that the dispersion is negligible. However, to improve our understanding of the possible interaction of insulin and rivastigmine, or its target AChE, more in silico modelling and in vivo studies are needed.Tahereh Jamshidnejad-TosaramandaniSoheila KashanianMahsa BabaeiMohamed H. Al-SabriHelgi B. SchiöthMDPI AGarticleAlzheimer diseaseinsulinrivastigmineacetylcholinesterase inhibitioncombinational therapyMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1136, p 1136 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Alzheimer disease insulin rivastigmine acetylcholinesterase inhibition combinational therapy Medicine R Pharmacy and materia medica RS1-441
spellingShingle	Alzheimer disease insulin rivastigmine acetylcholinesterase inhibition combinational therapy Medicine R Pharmacy and materia medica RS1-441 Tahereh Jamshidnejad-Tosaramandani Soheila Kashanian Mahsa Babaei Mohamed H. Al-Sabri Helgi B. Schiöth The Potential Effect of Insulin on AChE and Its Interactions with Rivastigmine In Vitro
description	There is no definite cure for Alzheimer’s disease (AD) due to its multifactorial origin. Drugs that inhibit acetylcholinesterase (AChE), such as rivastigmine, are promising symptomatic treatments for AD. Emerging evidence suggests that insulin therapy can hinder several aspects of AD pathology. Insulin has been shown to modify the activity of AChE, but it is still unknown how insulin and AChE interact. Combination therapy, which targets several features of the disease based on existing medications, can provide a worthy therapy option for AD management. However, to date, no studies have examined the potential interaction of insulin with AChE and/or rivastigmine in vitro. In the present study, we employed the Response Surface Methodology (RSM) as an in vitro assessment to investigate the effect of insulin on both AChE activity and rivastigmine inhibitory action using a common spectrophotometric assay for cholinesterase activity, Ellman’s method. Our results showed that insulin, even at high concentrations, has an insignificant effect on both the activity of AChE and rivastigmine’s inhibitory action. The variance of our data is near zero, which means that the dispersion is negligible. However, to improve our understanding of the possible interaction of insulin and rivastigmine, or its target AChE, more in silico modelling and in vivo studies are needed.
format	article
author	Tahereh Jamshidnejad-Tosaramandani Soheila Kashanian Mahsa Babaei Mohamed H. Al-Sabri Helgi B. Schiöth
author_facet	Tahereh Jamshidnejad-Tosaramandani Soheila Kashanian Mahsa Babaei Mohamed H. Al-Sabri Helgi B. Schiöth
author_sort	Tahereh Jamshidnejad-Tosaramandani
title	The Potential Effect of Insulin on AChE and Its Interactions with Rivastigmine In Vitro
title_short	The Potential Effect of Insulin on AChE and Its Interactions with Rivastigmine In Vitro
title_full	The Potential Effect of Insulin on AChE and Its Interactions with Rivastigmine In Vitro
title_fullStr	The Potential Effect of Insulin on AChE and Its Interactions with Rivastigmine In Vitro
title_full_unstemmed	The Potential Effect of Insulin on AChE and Its Interactions with Rivastigmine In Vitro
title_sort	potential effect of insulin on ache and its interactions with rivastigmine in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/92921d97535b4d4ea6eb9d82c6c12859
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The Potential Effect of Insulin on AChE and Its Interactions with Rivastigmine In Vitro

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