Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis

Abstract We present the first data describing molecular evidence of planktonic coenzyme factor 430 (hereafter referred to as F430), a key signature of methanogenic archaea, obtained from a water column sample in a freshwater lake in Japan. We report concentrations of native F430 ranging from 6.8–35 ...

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Autores principales:	Atsushi Urai, Makoto Matsushita, Ho-Dong Park, Hiroyuki Imachi, Miyuki Ogawara, Hiroki Iwata, Masanori Kaneko, Nanako O. Ogawa, Naohiko Ohkouchi, Yoshinori Takano
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Planktonic methanogen Coenzyme F430 mcrA Lake Suwa Surface hydrosphere Geography. Anthropology. Recreation G Geology QE1-996.5
Acceso en línea:	https://doaj.org/article/929b8e60fa194550bca85eaeb2fb1c0f
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spelling	oai:doaj.org-article:929b8e60fa194550bca85eaeb2fb1c0f2021-11-07T12:17:18ZDetection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis10.1186/s40645-021-00450-72197-4284https://doaj.org/article/929b8e60fa194550bca85eaeb2fb1c0f2021-11-01T00:00:00Zhttps://doi.org/10.1186/s40645-021-00450-7https://doaj.org/toc/2197-4284Abstract We present the first data describing molecular evidence of planktonic coenzyme factor 430 (hereafter referred to as F430), a key signature of methanogenic archaea, obtained from a water column sample in a freshwater lake in Japan. We report concentrations of native F430 ranging from 6.8–35 × 102 femto mol g-wet−1 and 8.5–86 × 102 femto mol g-wet−1 in the water column and core-top sediment, respectively. Among these profiles, the epimer F430 of the deactivated chemical formula was very low overall in both planktonic and benthic environments. Small subunit (SSU) rRNA gene sequencing revealed that the planktonic microbial community was clearly different from that of the benthic sediment. Planktonic cyanobacteria (e.g., Microcystis) were predominant in the water column, whereas a wide variety of bacteria and archaea, including methanogens (e.g., Methanobacterium, Methanoregula, Methanothrix, Methanosarcina, and Methanocella), were observed in the core-top sediment. These results suggest that the planktonic cyanobacterial community may be rapidly degraded at the water–sediment interface driven by activated benthic microbial heterotrophic processes. Graphic abstractAtsushi UraiMakoto MatsushitaHo-Dong ParkHiroyuki ImachiMiyuki OgawaraHiroki IwataMasanori KanekoNanako O. OgawaNaohiko OhkouchiYoshinori TakanoSpringerOpenarticlePlanktonic methanogenCoenzyme F430mcrALake SuwaSurface hydrosphereGeography. Anthropology. RecreationGGeologyQE1-996.5ENProgress in Earth and Planetary Science, Vol 8, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Planktonic methanogen Coenzyme F430 mcrA Lake Suwa Surface hydrosphere Geography. Anthropology. Recreation G Geology QE1-996.5
spellingShingle	Planktonic methanogen Coenzyme F430 mcrA Lake Suwa Surface hydrosphere Geography. Anthropology. Recreation G Geology QE1-996.5 Atsushi Urai Makoto Matsushita Ho-Dong Park Hiroyuki Imachi Miyuki Ogawara Hiroki Iwata Masanori Kaneko Nanako O. Ogawa Naohiko Ohkouchi Yoshinori Takano Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis
description	Abstract We present the first data describing molecular evidence of planktonic coenzyme factor 430 (hereafter referred to as F430), a key signature of methanogenic archaea, obtained from a water column sample in a freshwater lake in Japan. We report concentrations of native F430 ranging from 6.8–35 × 102 femto mol g-wet−1 and 8.5–86 × 102 femto mol g-wet−1 in the water column and core-top sediment, respectively. Among these profiles, the epimer F430 of the deactivated chemical formula was very low overall in both planktonic and benthic environments. Small subunit (SSU) rRNA gene sequencing revealed that the planktonic microbial community was clearly different from that of the benthic sediment. Planktonic cyanobacteria (e.g., Microcystis) were predominant in the water column, whereas a wide variety of bacteria and archaea, including methanogens (e.g., Methanobacterium, Methanoregula, Methanothrix, Methanosarcina, and Methanocella), were observed in the core-top sediment. These results suggest that the planktonic cyanobacterial community may be rapidly degraded at the water–sediment interface driven by activated benthic microbial heterotrophic processes. Graphic abstract
format	article
author	Atsushi Urai Makoto Matsushita Ho-Dong Park Hiroyuki Imachi Miyuki Ogawara Hiroki Iwata Masanori Kaneko Nanako O. Ogawa Naohiko Ohkouchi Yoshinori Takano
author_facet	Atsushi Urai Makoto Matsushita Ho-Dong Park Hiroyuki Imachi Miyuki Ogawara Hiroki Iwata Masanori Kaneko Nanako O. Ogawa Naohiko Ohkouchi Yoshinori Takano
author_sort	Atsushi Urai
title	Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis
title_short	Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis
title_full	Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis
title_fullStr	Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis
title_full_unstemmed	Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis
title_sort	detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/929b8e60fa194550bca85eaeb2fb1c0f
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Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis

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