Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production

Abstract Aflatoxin is a group of polyketide-derived carcinogenic and mutagenic secondary metabolites produced by Aspergillus flavus that negatively impact global food security and threaten the health of both humans and livestock. Aflatoxin biosynthesis is strongly affected by the fungal developmenta...

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Autores principales:	Fei Tian, Sang Yoo Lee, So Young Woo, Hwa Young Choi, Seongeun Heo, Gyoungju Nah, Hyang Sook Chun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/92acc0098a1c4680ae2af28c76283999
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spelling	oai:doaj.org-article:92acc0098a1c4680ae2af28c762839992021-12-02T14:06:25ZTranscriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production10.1038/s41598-021-82488-72045-2322https://doaj.org/article/92acc0098a1c4680ae2af28c762839992021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82488-7https://doaj.org/toc/2045-2322Abstract Aflatoxin is a group of polyketide-derived carcinogenic and mutagenic secondary metabolites produced by Aspergillus flavus that negatively impact global food security and threaten the health of both humans and livestock. Aflatoxin biosynthesis is strongly affected by the fungal developmental stage, cultivation conditions, and environmental stress. In this study, a novel float culture method was used to examine the direct responses of the A. flavus transcriptome to temperature stress, oxidative stress, and their dual effects during the aflatoxin production stage. The transcriptomic response of A. flavus illustrated that the co-regulation of different secondary metabolic pathways likely contributes to maintaining cellular homeostasis and promoting cell survival under stress conditions. In particular, aflatoxin biosynthetic gene expression was downregulated, while genes encoding secondary metabolites with antioxidant properties, such as kojic acid and imizoquins, were upregulated under stress conditions. Multiple mitochondrial function-related genes, including those encoding NADH:ubiquinone oxidoreductase, ubiquinol-cytochrome C reductase, and alternative oxidase, were differentially expressed. These data can provide insights into the important mechanisms through which secondary metabolism in A. flavus is co-regulated and facilitate the deployment of various approaches for the effective control and prevention of aflatoxin contamination in food crops.Fei TianSang Yoo LeeSo Young WooHwa Young ChoiSeongeun HeoGyoungju NahHyang Sook ChunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fei Tian Sang Yoo Lee So Young Woo Hwa Young Choi Seongeun Heo Gyoungju Nah Hyang Sook Chun Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production
description	Abstract Aflatoxin is a group of polyketide-derived carcinogenic and mutagenic secondary metabolites produced by Aspergillus flavus that negatively impact global food security and threaten the health of both humans and livestock. Aflatoxin biosynthesis is strongly affected by the fungal developmental stage, cultivation conditions, and environmental stress. In this study, a novel float culture method was used to examine the direct responses of the A. flavus transcriptome to temperature stress, oxidative stress, and their dual effects during the aflatoxin production stage. The transcriptomic response of A. flavus illustrated that the co-regulation of different secondary metabolic pathways likely contributes to maintaining cellular homeostasis and promoting cell survival under stress conditions. In particular, aflatoxin biosynthetic gene expression was downregulated, while genes encoding secondary metabolites with antioxidant properties, such as kojic acid and imizoquins, were upregulated under stress conditions. Multiple mitochondrial function-related genes, including those encoding NADH:ubiquinone oxidoreductase, ubiquinol-cytochrome C reductase, and alternative oxidase, were differentially expressed. These data can provide insights into the important mechanisms through which secondary metabolism in A. flavus is co-regulated and facilitate the deployment of various approaches for the effective control and prevention of aflatoxin contamination in food crops.
format	article
author	Fei Tian Sang Yoo Lee So Young Woo Hwa Young Choi Seongeun Heo Gyoungju Nah Hyang Sook Chun
author_facet	Fei Tian Sang Yoo Lee So Young Woo Hwa Young Choi Seongeun Heo Gyoungju Nah Hyang Sook Chun
author_sort	Fei Tian
title	Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production
title_short	Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production
title_full	Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production
title_fullStr	Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production
title_full_unstemmed	Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production
title_sort	transcriptomic responses of aspergillus flavus to temperature and oxidative stresses during aflatoxin production
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/92acc0098a1c4680ae2af28c76283999
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Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production

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