Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control

Abstract In vitro spermatogenesis (IVS) using air–liquid interphase organ culture method is possible with mouse testis tissues. The same method, however, has been hardly applicable to animals other than mice, only producing no or limited progression of spermatogenesis. In the present study, we chall...

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Autores principales:	Takafumi Matsumura, Takuya Sato, Takeru Abe, Hiroyuki Sanjo, Kumiko Katagiri, Hiroshi Kimura, Teruo Fujii, Hiromitsu Tanaka, Masumi Hirabayashi, Takehiko Ogawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/92ad44ad0b874881b0c56413d201536a
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spelling	oai:doaj.org-article:92ad44ad0b874881b0c56413d201536a2021-12-02T13:30:22ZRat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control10.1038/s41598-021-82792-22045-2322https://doaj.org/article/92ad44ad0b874881b0c56413d201536a2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82792-2https://doaj.org/toc/2045-2322Abstract In vitro spermatogenesis (IVS) using air–liquid interphase organ culture method is possible with mouse testis tissues. The same method, however, has been hardly applicable to animals other than mice, only producing no or limited progression of spermatogenesis. In the present study, we challenged IVS of rats with modifications of culture medium, by supplementing chemical substances, including hormones, antioxidants, and lysophospholipids. In addition, reducing oxygen tension by placing tissues in an incubator of lower oxygen concentration and/or applying silicone cover ceiling on top of the tissue were effective for improving the spermatogenic efficiency. Through these modifications of the culture condition, rat spermatogenesis up to round spermatids was maintained over 70 days in the cultured tissue. Present results demonstrated a significant progress in rat IVS, revealing conditions commonly favorable for mice and rats as well as finding rat-specific optimizations. This is an important step towards successful IVS in many animal species, including humans.Takafumi MatsumuraTakuya SatoTakeru AbeHiroyuki SanjoKumiko KatagiriHiroshi KimuraTeruo FujiiHiromitsu TanakaMasumi HirabayashiTakehiko OgawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takafumi Matsumura Takuya Sato Takeru Abe Hiroyuki Sanjo Kumiko Katagiri Hiroshi Kimura Teruo Fujii Hiromitsu Tanaka Masumi Hirabayashi Takehiko Ogawa Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control
description	Abstract In vitro spermatogenesis (IVS) using air–liquid interphase organ culture method is possible with mouse testis tissues. The same method, however, has been hardly applicable to animals other than mice, only producing no or limited progression of spermatogenesis. In the present study, we challenged IVS of rats with modifications of culture medium, by supplementing chemical substances, including hormones, antioxidants, and lysophospholipids. In addition, reducing oxygen tension by placing tissues in an incubator of lower oxygen concentration and/or applying silicone cover ceiling on top of the tissue were effective for improving the spermatogenic efficiency. Through these modifications of the culture condition, rat spermatogenesis up to round spermatids was maintained over 70 days in the cultured tissue. Present results demonstrated a significant progress in rat IVS, revealing conditions commonly favorable for mice and rats as well as finding rat-specific optimizations. This is an important step towards successful IVS in many animal species, including humans.
format	article
author	Takafumi Matsumura Takuya Sato Takeru Abe Hiroyuki Sanjo Kumiko Katagiri Hiroshi Kimura Teruo Fujii Hiromitsu Tanaka Masumi Hirabayashi Takehiko Ogawa
author_facet	Takafumi Matsumura Takuya Sato Takeru Abe Hiroyuki Sanjo Kumiko Katagiri Hiroshi Kimura Teruo Fujii Hiromitsu Tanaka Masumi Hirabayashi Takehiko Ogawa
author_sort	Takafumi Matsumura
title	Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control
title_short	Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control
title_full	Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control
title_fullStr	Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control
title_full_unstemmed	Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control
title_sort	rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/92ad44ad0b874881b0c56413d201536a
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Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control

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