Silencing of KCNQ1OT1 Decreases Oxidative Stress and Pyroptosis of Renal Tubular Epithelial Cells

Bei Zhu,1 Xingbo Cheng,2 Yilan Jiang,3 Ming Cheng,4 Luping Chen,3 Jiajun Bao,3 Xiaofeng Tang3 1Department of Endocrinology, The First Affiliated Hospital of Soochow University, Suzhou 215006, People’s Republic of China, Department of Endocrinology, Rugao People’s Hospital, Nanton...

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Autores principales:	Zhu B, Cheng X, Jiang Y, Cheng M, Chen L, Bao J, Tang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	lncrna kcnq1ot1 oxidative stress pyroptosis diabetic nephropathy renal tubular epithelial cells Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/92af5e9e89074f9aba834be125a0ad2d
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spelling	oai:doaj.org-article:92af5e9e89074f9aba834be125a0ad2d2021-12-02T03:32:52ZSilencing of KCNQ1OT1 Decreases Oxidative Stress and Pyroptosis of Renal Tubular Epithelial Cells1178-7007https://doaj.org/article/92af5e9e89074f9aba834be125a0ad2d2020-02-01T00:00:00Zhttps://www.dovepress.com/silencing-of-kcnq1ot1-decreases-oxidative-stress-and-pyroptosis-of-ren-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Bei Zhu,1 Xingbo Cheng,2 Yilan Jiang,3 Ming Cheng,4 Luping Chen,3 Jiajun Bao,3 Xiaofeng Tang3 1Department of Endocrinology, The First Affiliated Hospital of Soochow University, Suzhou 215006, People’s Republic of China, Department of Endocrinology, Rugao People’s Hospital, Nantong 226500, People’s Republic of China; 2Department of Endocrinology, The First Affiliated Hospital of Soochow University, Suzhou 215006, People’s Republic of China; 3Department of Endocrinology, Rugao People’s Hospital, Nantong 226500, People’s Republic of China; 4School of Rail Transportation, Soochow University, Suzhou 215131, People’s Republic of ChinaCorrespondence: Xingbo ChengDepartment of Endocrinology, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, People’s Republic of ChinaEmail xbcheng1107@163.comMing ChengSchool of Rail Transportation, Soochow University, 8 Ji Xue Road., Xiangcheng District, Suzhou 215131, People’s Republic of ChinaEmail mchengcm@163.comBackground: Long noncoding RNAs (lncRNAs) can regulate the progression of DN. This research aimed to study the effect of lncRNA KCNQ1OT1 on the oxidative stress and pyroptosis of the renal tubular epithelial cells induced by high glucose (HG).Methods: RT-qPCR analysis detected the KCNQ1OT1 expression in serum with DN and HG-induced HK-2 cells, detect the expression of NLRP3, cleaved-caspase1, P-caspase1, IL-1β, p-IL-1β and GSDMD-N in HG-induced HK-2 cells, and confirm the transfection effects. The expression of NLRP3, cleaved-caspase1, P-caspase1, IL-1β, p-IL-1β and GSDMD-N in HG-induced HK-2 cells was also analyzed by Western blot analysis. ELISA assay detected the levels of TNF-α, IL-6 and MCP-1. The levels of ROS, MDA and SOD were determined by respective ELISA kits and ROS was also detected by the ROS assay kit (containing DCFH-DA).Results: We found that KCNQ1OT1 was increased in the plasma of patients with DN and HG-induced HK-2 cells and KCNQ1OT1 interference could decrease the inflammation, oxidative stress and pyroptosis of HG-induced HK-2 cells. In addition, KCNQ1OT1 directly targets miR-506-3p. MiR-506-3p was downregulated in the plasma of patients with DN and HG-induced HK-2 cells and KCNQ1OT1 interference promoted the expression of miR-506-3p. MiR-506-3p overexpression suppressed the inflammation, oxidative stress and pyroptosis of HG-induced HK-2 cells.Conclusion: This study demonstrated that downregulation of KCNQ1OT1 inhibited the inflammation, oxidative stress and pyroptosis of HG-induced HK-2 cells by up-regulating the expression of miR-506-3p, which provide new insights into the treatment of DN.Keywords: LncRNA KCNQ1OT1, oxidative stress, pyroptosis, diabetic nephropathy, renal tubular epithelial cellsZhu BCheng XJiang YCheng MChen LBao JTang XDove Medical Pressarticlelncrna kcnq1ot1oxidative stresspyroptosisdiabetic nephropathyrenal tubular epithelial cellsSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 365-375 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	lncrna kcnq1ot1 oxidative stress pyroptosis diabetic nephropathy renal tubular epithelial cells Specialties of internal medicine RC581-951
spellingShingle	lncrna kcnq1ot1 oxidative stress pyroptosis diabetic nephropathy renal tubular epithelial cells Specialties of internal medicine RC581-951 Zhu B Cheng X Jiang Y Cheng M Chen L Bao J Tang X Silencing of KCNQ1OT1 Decreases Oxidative Stress and Pyroptosis of Renal Tubular Epithelial Cells
description	Bei Zhu,1 Xingbo Cheng,2 Yilan Jiang,3 Ming Cheng,4 Luping Chen,3 Jiajun Bao,3 Xiaofeng Tang3 1Department of Endocrinology, The First Affiliated Hospital of Soochow University, Suzhou 215006, People’s Republic of China, Department of Endocrinology, Rugao People’s Hospital, Nantong 226500, People’s Republic of China; 2Department of Endocrinology, The First Affiliated Hospital of Soochow University, Suzhou 215006, People’s Republic of China; 3Department of Endocrinology, Rugao People’s Hospital, Nantong 226500, People’s Republic of China; 4School of Rail Transportation, Soochow University, Suzhou 215131, People’s Republic of ChinaCorrespondence: Xingbo ChengDepartment of Endocrinology, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, People’s Republic of ChinaEmail xbcheng1107@163.comMing ChengSchool of Rail Transportation, Soochow University, 8 Ji Xue Road., Xiangcheng District, Suzhou 215131, People’s Republic of ChinaEmail mchengcm@163.comBackground: Long noncoding RNAs (lncRNAs) can regulate the progression of DN. This research aimed to study the effect of lncRNA KCNQ1OT1 on the oxidative stress and pyroptosis of the renal tubular epithelial cells induced by high glucose (HG).Methods: RT-qPCR analysis detected the KCNQ1OT1 expression in serum with DN and HG-induced HK-2 cells, detect the expression of NLRP3, cleaved-caspase1, P-caspase1, IL-1β, p-IL-1β and GSDMD-N in HG-induced HK-2 cells, and confirm the transfection effects. The expression of NLRP3, cleaved-caspase1, P-caspase1, IL-1β, p-IL-1β and GSDMD-N in HG-induced HK-2 cells was also analyzed by Western blot analysis. ELISA assay detected the levels of TNF-α, IL-6 and MCP-1. The levels of ROS, MDA and SOD were determined by respective ELISA kits and ROS was also detected by the ROS assay kit (containing DCFH-DA).Results: We found that KCNQ1OT1 was increased in the plasma of patients with DN and HG-induced HK-2 cells and KCNQ1OT1 interference could decrease the inflammation, oxidative stress and pyroptosis of HG-induced HK-2 cells. In addition, KCNQ1OT1 directly targets miR-506-3p. MiR-506-3p was downregulated in the plasma of patients with DN and HG-induced HK-2 cells and KCNQ1OT1 interference promoted the expression of miR-506-3p. MiR-506-3p overexpression suppressed the inflammation, oxidative stress and pyroptosis of HG-induced HK-2 cells.Conclusion: This study demonstrated that downregulation of KCNQ1OT1 inhibited the inflammation, oxidative stress and pyroptosis of HG-induced HK-2 cells by up-regulating the expression of miR-506-3p, which provide new insights into the treatment of DN.Keywords: LncRNA KCNQ1OT1, oxidative stress, pyroptosis, diabetic nephropathy, renal tubular epithelial cells
format	article
author	Zhu B Cheng X Jiang Y Cheng M Chen L Bao J Tang X
author_facet	Zhu B Cheng X Jiang Y Cheng M Chen L Bao J Tang X
author_sort	Zhu B
title	Silencing of KCNQ1OT1 Decreases Oxidative Stress and Pyroptosis of Renal Tubular Epithelial Cells
title_short	Silencing of KCNQ1OT1 Decreases Oxidative Stress and Pyroptosis of Renal Tubular Epithelial Cells
title_full	Silencing of KCNQ1OT1 Decreases Oxidative Stress and Pyroptosis of Renal Tubular Epithelial Cells
title_fullStr	Silencing of KCNQ1OT1 Decreases Oxidative Stress and Pyroptosis of Renal Tubular Epithelial Cells
title_full_unstemmed	Silencing of KCNQ1OT1 Decreases Oxidative Stress and Pyroptosis of Renal Tubular Epithelial Cells
title_sort	silencing of kcnq1ot1 decreases oxidative stress and pyroptosis of renal tubular epithelial cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/92af5e9e89074f9aba834be125a0ad2d
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Silencing of KCNQ1OT1 Decreases Oxidative Stress and Pyroptosis of Renal Tubular Epithelial Cells

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