Dataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and DYT-TOR1A mouse model of dystonia, basally and during stress

Here, we present quantitative subcellular compartment-specific proteomic data from wildtype and DYT-TOR1A heterozygous mouse embryonic fibroblasts (MEFs) basally and following thapsigargin (Tg) treatment [1]. In this experiment, we generated MEFs from wild type (WT) and a heterozygous DYT-TOR1A mous...

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Autores principales:	Kunal Shroff, Zachary F. Caffall, Erik J. Soderblom, Greg Waitt, Tricia Ho, Nicole Calakos
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Dystonia TorsinA Proteomics Subcellular fractionation Thapsigargin Stress response Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/92b04d4359d04b72a79daef5697143b3
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spelling	oai:doaj.org-article:92b04d4359d04b72a79daef5697143b32021-12-02T05:01:50ZDataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and DYT-TOR1A mouse model of dystonia, basally and during stress2352-340910.1016/j.dib.2021.107609https://doaj.org/article/92b04d4359d04b72a79daef5697143b32021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352340921008842https://doaj.org/toc/2352-3409Here, we present quantitative subcellular compartment-specific proteomic data from wildtype and DYT-TOR1A heterozygous mouse embryonic fibroblasts (MEFs) basally and following thapsigargin (Tg) treatment [1]. In this experiment, we generated MEFs from wild type (WT) and a heterozygous DYT-TOR1A mouse model of dystonia. Subsequently, these MEF cultures were treated with either 1 µM Tg or dimethylsulfoxide vehicle (Veh) for six hours. Following treatment, the cells were fractionated into nuclear and cytosolic fractions. Liquid chromatography, tandem mass spectrometry (LC/MS/MS)-based proteomic profiling identified 65,056 unique peptides and 4801 unique proteins across all samples. The data presented here provide subcellular compartment-specific proteomic information within a dystonia model system both basally and under cellular stress. These data can inform future experiments focused on studying the function of TorsinA, the protein encoded by TOR1A, and its potential role in nucleocytoplasmic transport and proteostasis. In addition, the information in this article can also inform future mechanistic studies investigating the relationship between DYT-TOR1A dystonia and the cellular stress response to advance understanding of the pathogenesis of dystonia.Kunal ShroffZachary F. CaffallErik J. SoderblomGreg WaittTricia HoNicole CalakosElsevierarticleDystoniaTorsinAProteomicsSubcellular fractionationThapsigarginStress responseComputer applications to medicine. Medical informaticsR858-859.7Science (General)Q1-390ENData in Brief, Vol 39, Iss , Pp 107609- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dystonia TorsinA Proteomics Subcellular fractionation Thapsigargin Stress response Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390
spellingShingle	Dystonia TorsinA Proteomics Subcellular fractionation Thapsigargin Stress response Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390 Kunal Shroff Zachary F. Caffall Erik J. Soderblom Greg Waitt Tricia Ho Nicole Calakos Dataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and DYT-TOR1A mouse model of dystonia, basally and during stress
description	Here, we present quantitative subcellular compartment-specific proteomic data from wildtype and DYT-TOR1A heterozygous mouse embryonic fibroblasts (MEFs) basally and following thapsigargin (Tg) treatment [1]. In this experiment, we generated MEFs from wild type (WT) and a heterozygous DYT-TOR1A mouse model of dystonia. Subsequently, these MEF cultures were treated with either 1 µM Tg or dimethylsulfoxide vehicle (Veh) for six hours. Following treatment, the cells were fractionated into nuclear and cytosolic fractions. Liquid chromatography, tandem mass spectrometry (LC/MS/MS)-based proteomic profiling identified 65,056 unique peptides and 4801 unique proteins across all samples. The data presented here provide subcellular compartment-specific proteomic information within a dystonia model system both basally and under cellular stress. These data can inform future experiments focused on studying the function of TorsinA, the protein encoded by TOR1A, and its potential role in nucleocytoplasmic transport and proteostasis. In addition, the information in this article can also inform future mechanistic studies investigating the relationship between DYT-TOR1A dystonia and the cellular stress response to advance understanding of the pathogenesis of dystonia.
format	article
author	Kunal Shroff Zachary F. Caffall Erik J. Soderblom Greg Waitt Tricia Ho Nicole Calakos
author_facet	Kunal Shroff Zachary F. Caffall Erik J. Soderblom Greg Waitt Tricia Ho Nicole Calakos
author_sort	Kunal Shroff
title	Dataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and DYT-TOR1A mouse model of dystonia, basally and during stress
title_short	Dataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and DYT-TOR1A mouse model of dystonia, basally and during stress
title_full	Dataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and DYT-TOR1A mouse model of dystonia, basally and during stress
title_fullStr	Dataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and DYT-TOR1A mouse model of dystonia, basally and during stress
title_full_unstemmed	Dataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and DYT-TOR1A mouse model of dystonia, basally and during stress
title_sort	dataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and dyt-tor1a mouse model of dystonia, basally and during stress
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/92b04d4359d04b72a79daef5697143b3
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Dataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and DYT-TOR1A mouse model of dystonia, basally and during stress

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