Comparison and validation of a fast method for the extraction and quantification of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids

Abstract: Background: Omega 3 family fatty acids are currently widely studied for the diverse effects they have on cardiovascular disease risk factors. Seafood is the main natural source of these compounds. Objetives: In this paper the accuracy is verified on the fat extraction in fish, between...

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Autores principales:	Alejandro Oviedo-Castrillón, Natalia Quintero-Laverde, Claudia Velásquez-Rodríguez, Beatriz Estella López-Marín
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia
Materias:	Fatty acids gas chromatography Bligh & Dyer EPA DHA omega 3 Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/92ba22747ea84312942426c597f82f6a
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spelling	oai:doaj.org-article:92ba22747ea84312942426c597f82f6a2021-12-02T07:01:15ZComparison and validation of a fast method for the extraction and quantification of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids0121-400410.17533/udea.vitae.v23n3a05https://doaj.org/article/92ba22747ea84312942426c597f82f6ahttp://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-40042016000300194&lng=en&tlng=enhttps://doaj.org/toc/0121-4004Abstract: Background: Omega 3 family fatty acids are currently widely studied for the diverse effects they have on cardiovascular disease risk factors. Seafood is the main natural source of these compounds. Objetives: In this paper the accuracy is verified on the fat extraction in fish, between two methods, using one of the most employees as it is the Bligh & Dyer since 1959, compared with SOXTEC, one more updated method with more modern equipment and validated quantification method for some omega 3 fatty acids by gas chromatography. Methods: Fat extraction was performed by SOXTEC and Bligh & Dyer with the use of a Certified Reference Material (SRM 1946). After having assessed the accuracy of both systems, a quantification method of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids was validated through gas chromatography. Results: It was found that the method proposed by Bligh & Dyer in 1959 is the most accurate because it showed better recovery percentages. Also, it was found that the quantification validation method of the most important omega 3 fatty acids complies with every parameter assessed throughout the validation. Conclusions: In conclusion SOXTEC being a more modern method which uses the latest technology, the Bligh & Dyer is the most effective method for removing fat in fish and gas chromatography allows quantification of fatty acids with precision and accuracy as meets the evaluated parameters.Alejandro Oviedo-CastrillónNatalia Quintero-LaverdeClaudia Velásquez-RodríguezBeatriz Estella López-MarínUniversidad de AntioquiaarticleFatty acidsgas chromatographyBligh & DyerEPADHAomega 3Food processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 23, Iss 3, Pp 194-201
institution	DOAJ
collection	DOAJ
language	EN
topic	Fatty acids gas chromatography Bligh & Dyer EPA DHA omega 3 Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Fatty acids gas chromatography Bligh & Dyer EPA DHA omega 3 Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Alejandro Oviedo-Castrillón Natalia Quintero-Laverde Claudia Velásquez-Rodríguez Beatriz Estella López-Marín Comparison and validation of a fast method for the extraction and quantification of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids
description	Abstract: Background: Omega 3 family fatty acids are currently widely studied for the diverse effects they have on cardiovascular disease risk factors. Seafood is the main natural source of these compounds. Objetives: In this paper the accuracy is verified on the fat extraction in fish, between two methods, using one of the most employees as it is the Bligh & Dyer since 1959, compared with SOXTEC, one more updated method with more modern equipment and validated quantification method for some omega 3 fatty acids by gas chromatography. Methods: Fat extraction was performed by SOXTEC and Bligh & Dyer with the use of a Certified Reference Material (SRM 1946). After having assessed the accuracy of both systems, a quantification method of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids was validated through gas chromatography. Results: It was found that the method proposed by Bligh & Dyer in 1959 is the most accurate because it showed better recovery percentages. Also, it was found that the quantification validation method of the most important omega 3 fatty acids complies with every parameter assessed throughout the validation. Conclusions: In conclusion SOXTEC being a more modern method which uses the latest technology, the Bligh & Dyer is the most effective method for removing fat in fish and gas chromatography allows quantification of fatty acids with precision and accuracy as meets the evaluated parameters.
format	article
author	Alejandro Oviedo-Castrillón Natalia Quintero-Laverde Claudia Velásquez-Rodríguez Beatriz Estella López-Marín
author_facet	Alejandro Oviedo-Castrillón Natalia Quintero-Laverde Claudia Velásquez-Rodríguez Beatriz Estella López-Marín
author_sort	Alejandro Oviedo-Castrillón
title	Comparison and validation of a fast method for the extraction and quantification of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids
title_short	Comparison and validation of a fast method for the extraction and quantification of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids
title_full	Comparison and validation of a fast method for the extraction and quantification of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids
title_fullStr	Comparison and validation of a fast method for the extraction and quantification of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids
title_full_unstemmed	Comparison and validation of a fast method for the extraction and quantification of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids
title_sort	comparison and validation of a fast method for the extraction and quantification of α-linolenic, eicosapentaenoic, and docosahexaenoic fatty acids
publisher	Universidad de Antioquia
url	https://doaj.org/article/92ba22747ea84312942426c597f82f6a
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Comparison and validation of a fast method for the extraction and quantification of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids

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