λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.

Using phages is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. Apoptin, a protein from chicken anemia virus (CAV) has the ability to specificall...

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Autores principales:	Alireza Shoae-Hassani, Peyman Keyhanvar, Alexander Marcus Seifalian, Seyed Abdolreza Mortazavi-Tabatabaei, Narmin Ghaderi, Khosro Issazadeh, Nour Amirmozafari, Javad Verdi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/92cc5f32b1144f7c937647ea317acf76
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spelling	oai:doaj.org-article:92cc5f32b1144f7c937647ea317acf762021-11-18T08:45:04Zλ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.1932-620310.1371/journal.pone.0079907https://doaj.org/article/92cc5f32b1144f7c937647ea317acf762013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278212/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Using phages is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. Apoptin, a protein from chicken anemia virus (CAV) has the ability to specifically induce apoptosis only in carcinoma cells. We presented a safe method of breast tumor therapy via the apoptin expressing λ NBPs. Here, we constructed a λ ZAP-CMV-apoptin recombinant NBP and investigated the effectiveness of its apoptotic activity on BT-474, MDA-MB-361, SKBR-3, UACC-812 and ZR-75 cell lines that over-expressing her-2 marker. Apoptosis was evaluated via annexin-V fluorescent iso-thiocyanate/propidium iodide staining, flow-cytometric method and TUNEL assay. Transfection with NBPs carrying λ ZAP-CMV-apoptin significantly inhibited growth of all the breast carcinoma cell lines in vitro. Also nude mice model implanted BT-474 human breast tumor was successfully responded to the systemic and local injection of untargeted recombinant λ NBPs. The results presented here reveal important features of recombinant λ nanobioparticles to serve as safe delivery and expression platform for human cancer therapy.Alireza Shoae-HassaniAlireza Shoae-HassaniPeyman KeyhanvarAlexander Marcus SeifalianSeyed Abdolreza Mortazavi-TabatabaeiNarmin GhaderiKhosro IssazadehNour AmirmozafariJavad VerdiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79907 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alireza Shoae-Hassani Alireza Shoae-Hassani Peyman Keyhanvar Alexander Marcus Seifalian Seyed Abdolreza Mortazavi-Tabatabaei Narmin Ghaderi Khosro Issazadeh Nour Amirmozafari Javad Verdi λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.
description	Using phages is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. Apoptin, a protein from chicken anemia virus (CAV) has the ability to specifically induce apoptosis only in carcinoma cells. We presented a safe method of breast tumor therapy via the apoptin expressing λ NBPs. Here, we constructed a λ ZAP-CMV-apoptin recombinant NBP and investigated the effectiveness of its apoptotic activity on BT-474, MDA-MB-361, SKBR-3, UACC-812 and ZR-75 cell lines that over-expressing her-2 marker. Apoptosis was evaluated via annexin-V fluorescent iso-thiocyanate/propidium iodide staining, flow-cytometric method and TUNEL assay. Transfection with NBPs carrying λ ZAP-CMV-apoptin significantly inhibited growth of all the breast carcinoma cell lines in vitro. Also nude mice model implanted BT-474 human breast tumor was successfully responded to the systemic and local injection of untargeted recombinant λ NBPs. The results presented here reveal important features of recombinant λ nanobioparticles to serve as safe delivery and expression platform for human cancer therapy.
format	article
author	Alireza Shoae-Hassani Alireza Shoae-Hassani Peyman Keyhanvar Alexander Marcus Seifalian Seyed Abdolreza Mortazavi-Tabatabaei Narmin Ghaderi Khosro Issazadeh Nour Amirmozafari Javad Verdi
author_facet	Alireza Shoae-Hassani Alireza Shoae-Hassani Peyman Keyhanvar Alexander Marcus Seifalian Seyed Abdolreza Mortazavi-Tabatabaei Narmin Ghaderi Khosro Issazadeh Nour Amirmozafari Javad Verdi
author_sort	Alireza Shoae-Hassani
title	λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.
title_short	λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.
title_full	λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.
title_fullStr	λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.
title_full_unstemmed	λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.
title_sort	λ phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/92cc5f32b1144f7c937647ea317acf76
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λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.

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