In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes

Juan Sebastián Pappalardo,1–3 Cecilia A Langellotti,2 Sebastián Di Giacomo,1 Valeria Olivera,1 Valeria Quattrocchi,2 Patricia I Zamorano,1,2 William C Hartner,3 Tatyana S Levchenko,3 Vladimir P Torchilin3 1Virology Institute, Center for Research in Veterinary and Agr...

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Autores principales:	Pappalardo JS, Langellotti CA, Di Giacomo S, Olivera V, Quattrocchi V, Zamorano PI, Hartner WC, Levchenko TS, Torchilin VP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/92cd4ac4d34d4c1fb7c7362b10625d1b
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spelling	oai:doaj.org-article:92cd4ac4d34d4c1fb7c7362b10625d1b2021-12-02T04:47:02ZIn vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes1178-2013https://doaj.org/article/92cd4ac4d34d4c1fb7c7362b10625d1b2014-02-01T00:00:00Zhttp://www.dovepress.com/in-vitro-transfection-of-bone-marrow-derived-dendritic-cells-with-tatp-a15809https://doaj.org/toc/1178-2013 Juan Sebastián Pappalardo,1–3 Cecilia A Langellotti,2 Sebastián Di Giacomo,1 Valeria Olivera,1 Valeria Quattrocchi,2 Patricia I Zamorano,1,2 William C Hartner,3 Tatyana S Levchenko,3 Vladimir P Torchilin3 1Virology Institute, Center for Research in Veterinary and Agronomic Sciences, National Institute for Agricultural Technology (INTA), Hurlingham, BA, Argentina; 2National Council for Scientific and Technical Research (CONICET), Autonomous City of Buenos Aires, Argentina; 3Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USA Abstract: Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6. Keywords: dendritic cell transfection, green fluorescent protein, bovine herpes virus 1 glycoprotein D, liposomes, TAT peptide, interleukin 6Pappalardo JSLangellotti CADi Giacomo SOlivera VQuattrocchi VZamorano PIHartner WCLevchenko TSTorchilin VPDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 963-973 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Pappalardo JS Langellotti CA Di Giacomo S Olivera V Quattrocchi V Zamorano PI Hartner WC Levchenko TS Torchilin VP In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
description	Juan Sebastián Pappalardo,1–3 Cecilia A Langellotti,2 Sebastián Di Giacomo,1 Valeria Olivera,1 Valeria Quattrocchi,2 Patricia I Zamorano,1,2 William C Hartner,3 Tatyana S Levchenko,3 Vladimir P Torchilin3 1Virology Institute, Center for Research in Veterinary and Agronomic Sciences, National Institute for Agricultural Technology (INTA), Hurlingham, BA, Argentina; 2National Council for Scientific and Technical Research (CONICET), Autonomous City of Buenos Aires, Argentina; 3Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USA Abstract: Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6. Keywords: dendritic cell transfection, green fluorescent protein, bovine herpes virus 1 glycoprotein D, liposomes, TAT peptide, interleukin 6
format	article
author	Pappalardo JS Langellotti CA Di Giacomo S Olivera V Quattrocchi V Zamorano PI Hartner WC Levchenko TS Torchilin VP
author_facet	Pappalardo JS Langellotti CA Di Giacomo S Olivera V Quattrocchi V Zamorano PI Hartner WC Levchenko TS Torchilin VP
author_sort	Pappalardo JS
title	In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_short	In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_full	In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_fullStr	In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_full_unstemmed	In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_sort	in vitro transfection of bone marrow-derived dendritic cells with tatp-liposomes
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/92cd4ac4d34d4c1fb7c7362b10625d1b
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In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes

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