Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide

With their circumneutral pH and their moderate temperature (66 and 68°C, respectively), As Burgas and Muiño da Veiga are two important human-use hot springs, previously studied with traditional culture methods, but never explored with a metagenomic approach. In the present study, we have performed m...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	María-Eugenia DeCastro, Juan-José Escuder-Rodríguez, Manuel Becerra, Esther Rodríguez-Belmonte, María-Isabel González-Siso
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	thermophiles extreme environments hot springs microbial communities metagenomics next generation sequencing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/92d0b11bed964bce84e26eb98cd121f4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:92d0b11bed964bce84e26eb98cd121f4
record_format	dspace
spelling	oai:doaj.org-article:92d0b11bed964bce84e26eb98cd121f42021-12-01T07:05:27ZComparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide1664-302X10.3389/fmicb.2021.769065https://doaj.org/article/92d0b11bed964bce84e26eb98cd121f42021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.769065/fullhttps://doaj.org/toc/1664-302XWith their circumneutral pH and their moderate temperature (66 and 68°C, respectively), As Burgas and Muiño da Veiga are two important human-use hot springs, previously studied with traditional culture methods, but never explored with a metagenomic approach. In the present study, we have performed metagenomic sequence-based analyses to compare the taxonomic composition and functional potential of these hot springs. Proteobacteria, Deinococcus-Thermus, Firmicutes, Nitrospirae, and Aquificae are the dominant phyla in both geothermal springs, but there is a significant difference in the abundance of these phyla between As Burgas and Muiño da Veiga. Phylum Proteobacteria dominates As Burgas ecosystem while Aquificae is the most abundant phylum in Muiño da Veiga. Taxonomic and functional analyses reveal that the variability in water geochemistry might be shaping the differences in the microbial communities inhabiting these geothermal springs. The content in organic compounds of As Burgas water promotes the presence of heterotrophic populations of the genera Acidovorax and Thermus, whereas the sulfate-rich water of Muiño da Veiga favors the co-dominance of genera Sulfurihydrogenibium and Thermodesulfovibrio. Differences in ammonia concentration exert a selective pressure toward the growth of nitrogen-fixing bacteria such as Thermodesulfovibrio in Muiño da Veiga. Temperature and pH are two important factors shaping hot springs microbial communities as was determined by comparative analysis with other thermal springs.María-Eugenia DeCastroJuan-José Escuder-RodríguezManuel BecerraEsther Rodríguez-BelmonteMaría-Isabel González-SisoFrontiers Media S.A.articlethermophilesextreme environmentshot springsmicrobial communitiesmetagenomicsnext generation sequencingMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	thermophiles extreme environments hot springs microbial communities metagenomics next generation sequencing Microbiology QR1-502
spellingShingle	thermophiles extreme environments hot springs microbial communities metagenomics next generation sequencing Microbiology QR1-502 María-Eugenia DeCastro Juan-José Escuder-Rodríguez Manuel Becerra Esther Rodríguez-Belmonte María-Isabel González-Siso Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide
description	With their circumneutral pH and their moderate temperature (66 and 68°C, respectively), As Burgas and Muiño da Veiga are two important human-use hot springs, previously studied with traditional culture methods, but never explored with a metagenomic approach. In the present study, we have performed metagenomic sequence-based analyses to compare the taxonomic composition and functional potential of these hot springs. Proteobacteria, Deinococcus-Thermus, Firmicutes, Nitrospirae, and Aquificae are the dominant phyla in both geothermal springs, but there is a significant difference in the abundance of these phyla between As Burgas and Muiño da Veiga. Phylum Proteobacteria dominates As Burgas ecosystem while Aquificae is the most abundant phylum in Muiño da Veiga. Taxonomic and functional analyses reveal that the variability in water geochemistry might be shaping the differences in the microbial communities inhabiting these geothermal springs. The content in organic compounds of As Burgas water promotes the presence of heterotrophic populations of the genera Acidovorax and Thermus, whereas the sulfate-rich water of Muiño da Veiga favors the co-dominance of genera Sulfurihydrogenibium and Thermodesulfovibrio. Differences in ammonia concentration exert a selective pressure toward the growth of nitrogen-fixing bacteria such as Thermodesulfovibrio in Muiño da Veiga. Temperature and pH are two important factors shaping hot springs microbial communities as was determined by comparative analysis with other thermal springs.
format	article
author	María-Eugenia DeCastro Juan-José Escuder-Rodríguez Manuel Becerra Esther Rodríguez-Belmonte María-Isabel González-Siso
author_facet	María-Eugenia DeCastro Juan-José Escuder-Rodríguez Manuel Becerra Esther Rodríguez-Belmonte María-Isabel González-Siso
author_sort	María-Eugenia DeCastro
title	Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide
title_short	Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide
title_full	Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide
title_fullStr	Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide
title_full_unstemmed	Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide
title_sort	comparative metagenomic analysis of two hot springs from ourense (northwestern spain) and others worldwide
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/92d0b11bed964bce84e26eb98cd121f4
work_keys_str_mv	AT mariaeugeniadecastro comparativemetagenomicanalysisoftwohotspringsfromourensenorthwesternspainandothersworldwide AT juanjoseescuderrodriguez comparativemetagenomicanalysisoftwohotspringsfromourensenorthwesternspainandothersworldwide AT manuelbecerra comparativemetagenomicanalysisoftwohotspringsfromourensenorthwesternspainandothersworldwide AT estherrodriguezbelmonte comparativemetagenomicanalysisoftwohotspringsfromourensenorthwesternspainandothersworldwide AT mariaisabelgonzalezsiso comparativemetagenomicanalysisoftwohotspringsfromourensenorthwesternspainandothersworldwide
_version_	1718405426884640768

Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide

Ejemplares similares