CXCL14 facilitates the growth and metastasis of ovarian carcinoma cells via activation of the Wnt/β-catenin signaling pathway

Abstract Background There is an urgent need to identify potential targets in anticancer therapy to improve the survival and prognosis of patients with ovarian cancer (OC). Herein, we investigated the functional significance of chemokine (C-X-C motif) ligand 14 (CXCL14) in OC cell growth and epitheli...

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Autores principales:	Li-Na Gao, Man Hao, Xiao-Hui Liu, Li Zhang, Yan Dong, Yu-Fang Zhang, Xiao-Chun He
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	CXCL14 Ovarian cancer EMT Wnt/β-catenin Gynecology and obstetrics RG1-991
Acceso en línea:	https://doaj.org/article/92d1d4f2d9b34ae6bfe559242bc152fd
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spelling	oai:doaj.org-article:92d1d4f2d9b34ae6bfe559242bc152fd2021-11-21T12:32:15ZCXCL14 facilitates the growth and metastasis of ovarian carcinoma cells via activation of the Wnt/β-catenin signaling pathway10.1186/s13048-021-00913-x1757-2215https://doaj.org/article/92d1d4f2d9b34ae6bfe559242bc152fd2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13048-021-00913-xhttps://doaj.org/toc/1757-2215Abstract Background There is an urgent need to identify potential targets in anticancer therapy to improve the survival and prognosis of patients with ovarian cancer (OC). Herein, we investigated the functional significance of chemokine (C-X-C motif) ligand 14 (CXCL14) in OC cell growth and epithelial–mesenchymal transition (EMT). Methods qRT PCR and western blotting was used to detect CXCL14 mRNA level and protein expression, respectively. The functional mechanism of CXCL14 in OC was investigated by CCK-8, colony formation and transwell assays. The migration ability of OC cell was determined using wound healing. The protein expressions of CXCL14 and β-catenin in OC tissues were determined by immumohistochemical staining. Results We demonstrated that high levels of CXCL14 were associated with a worse prognosis in patients with OC. CXCL14 knockdown considerably restrained the growth, migration and invasion of OC cell in vitro. In contrast, ectopic CXCL14 overexpression yielded the opposite results. Investigations to determine the underlying molecular mechanisms revealed that the Wnt/β-catenin signaling pathway is involved in CXCL14-facilitated OC cell invasiveness. Conclusion These data collectively demonstrate that CXCL14 contributes to OC cell growth and metastatic potential by regulating the Wnt/β-catenin signaling pathway.Li-Na GaoMan HaoXiao-Hui LiuLi ZhangYan DongYu-Fang ZhangXiao-Chun HeBMCarticleCXCL14Ovarian cancerEMTWnt/β-cateninGynecology and obstetricsRG1-991ENJournal of Ovarian Research, Vol 14, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CXCL14 Ovarian cancer EMT Wnt/β-catenin Gynecology and obstetrics RG1-991
spellingShingle	CXCL14 Ovarian cancer EMT Wnt/β-catenin Gynecology and obstetrics RG1-991 Li-Na Gao Man Hao Xiao-Hui Liu Li Zhang Yan Dong Yu-Fang Zhang Xiao-Chun He CXCL14 facilitates the growth and metastasis of ovarian carcinoma cells via activation of the Wnt/β-catenin signaling pathway
description	Abstract Background There is an urgent need to identify potential targets in anticancer therapy to improve the survival and prognosis of patients with ovarian cancer (OC). Herein, we investigated the functional significance of chemokine (C-X-C motif) ligand 14 (CXCL14) in OC cell growth and epithelial–mesenchymal transition (EMT). Methods qRT PCR and western blotting was used to detect CXCL14 mRNA level and protein expression, respectively. The functional mechanism of CXCL14 in OC was investigated by CCK-8, colony formation and transwell assays. The migration ability of OC cell was determined using wound healing. The protein expressions of CXCL14 and β-catenin in OC tissues were determined by immumohistochemical staining. Results We demonstrated that high levels of CXCL14 were associated with a worse prognosis in patients with OC. CXCL14 knockdown considerably restrained the growth, migration and invasion of OC cell in vitro. In contrast, ectopic CXCL14 overexpression yielded the opposite results. Investigations to determine the underlying molecular mechanisms revealed that the Wnt/β-catenin signaling pathway is involved in CXCL14-facilitated OC cell invasiveness. Conclusion These data collectively demonstrate that CXCL14 contributes to OC cell growth and metastatic potential by regulating the Wnt/β-catenin signaling pathway.
format	article
author	Li-Na Gao Man Hao Xiao-Hui Liu Li Zhang Yan Dong Yu-Fang Zhang Xiao-Chun He
author_facet	Li-Na Gao Man Hao Xiao-Hui Liu Li Zhang Yan Dong Yu-Fang Zhang Xiao-Chun He
author_sort	Li-Na Gao
title	CXCL14 facilitates the growth and metastasis of ovarian carcinoma cells via activation of the Wnt/β-catenin signaling pathway
title_short	CXCL14 facilitates the growth and metastasis of ovarian carcinoma cells via activation of the Wnt/β-catenin signaling pathway
title_full	CXCL14 facilitates the growth and metastasis of ovarian carcinoma cells via activation of the Wnt/β-catenin signaling pathway
title_fullStr	CXCL14 facilitates the growth and metastasis of ovarian carcinoma cells via activation of the Wnt/β-catenin signaling pathway
title_full_unstemmed	CXCL14 facilitates the growth and metastasis of ovarian carcinoma cells via activation of the Wnt/β-catenin signaling pathway
title_sort	cxcl14 facilitates the growth and metastasis of ovarian carcinoma cells via activation of the wnt/β-catenin signaling pathway
publisher	BMC
publishDate	2021
url	https://doaj.org/article/92d1d4f2d9b34ae6bfe559242bc152fd
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CXCL14 facilitates the growth and metastasis of ovarian carcinoma cells via activation of the Wnt/β-catenin signaling pathway

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