Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.

We have investigated to what extent natural transformation acting on free DNA substrates can facilitate transfer of mobile elements including transposons, integrons and/or gene cassettes between bacterial species. Naturally transformable cells of Acinetobacter baylyi were exposed to DNA from integro...

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Autores principales:	Sara Domingues, Klaus Harms, W Florian Fricke, Pål J Johnsen, Gabriela J da Silva, Kaare Magne Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/92dcdc30f0994e77a5a1be035e52a244
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spelling	oai:doaj.org-article:92dcdc30f0994e77a5a1be035e52a2442021-11-18T06:04:08ZNatural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.1553-73661553-737410.1371/journal.ppat.1002837https://doaj.org/article/92dcdc30f0994e77a5a1be035e52a2442012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22876180/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374We have investigated to what extent natural transformation acting on free DNA substrates can facilitate transfer of mobile elements including transposons, integrons and/or gene cassettes between bacterial species. Naturally transformable cells of Acinetobacter baylyi were exposed to DNA from integron-carrying strains of the genera Acinetobacter, Citrobacter, Enterobacter, Escherichia, Pseudomonas, and Salmonella to determine the nature and frequency of transfer. Exposure to the various DNA sources resulted in acquisition of antibiotic resistance traits as well as entire integrons and transposons, over a 24 h exposure period. DNA incorporation was not solely dependent on integrase functions or the genetic relatedness between species. DNA sequence analyses revealed that several mechanisms facilitated stable integration in the recipient genome depending on the nature of the donor DNA; homologous or heterologous recombination and various types of transposition (Tn21-like and IS26-like). Both donor strains and transformed isolates were extensively characterized by antimicrobial susceptibility testing, integron- and cassette-specific PCRs, DNA sequencing, pulsed field gel electrophoreses (PFGE), Southern blot hybridizations, and by re-transformation assays. Two transformant strains were also genome-sequenced. Our data demonstrate that natural transformation facilitates interspecies transfer of genetic elements, suggesting that the transient presence of DNA in the cytoplasm may be sufficient for genomic integration to occur. Our study provides a plausible explanation for why sequence-conserved transposons, IS elements and integrons can be found disseminated among bacterial species. Moreover, natural transformation of integron harboring populations of competent bacteria revealed that interspecies exchange of gene cassettes can be highly efficient, and independent on genetic relatedness between donor and recipient. In conclusion, natural transformation provides a much broader capacity for horizontal acquisitions of genetic elements and hence, resistance traits from divergent species than previously assumed.Sara DominguesKlaus HarmsW Florian FrickePål J JohnsenGabriela J da SilvaKaare Magne NielsenPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 8, p e1002837 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Sara Domingues Klaus Harms W Florian Fricke Pål J Johnsen Gabriela J da Silva Kaare Magne Nielsen Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.
description	We have investigated to what extent natural transformation acting on free DNA substrates can facilitate transfer of mobile elements including transposons, integrons and/or gene cassettes between bacterial species. Naturally transformable cells of Acinetobacter baylyi were exposed to DNA from integron-carrying strains of the genera Acinetobacter, Citrobacter, Enterobacter, Escherichia, Pseudomonas, and Salmonella to determine the nature and frequency of transfer. Exposure to the various DNA sources resulted in acquisition of antibiotic resistance traits as well as entire integrons and transposons, over a 24 h exposure period. DNA incorporation was not solely dependent on integrase functions or the genetic relatedness between species. DNA sequence analyses revealed that several mechanisms facilitated stable integration in the recipient genome depending on the nature of the donor DNA; homologous or heterologous recombination and various types of transposition (Tn21-like and IS26-like). Both donor strains and transformed isolates were extensively characterized by antimicrobial susceptibility testing, integron- and cassette-specific PCRs, DNA sequencing, pulsed field gel electrophoreses (PFGE), Southern blot hybridizations, and by re-transformation assays. Two transformant strains were also genome-sequenced. Our data demonstrate that natural transformation facilitates interspecies transfer of genetic elements, suggesting that the transient presence of DNA in the cytoplasm may be sufficient for genomic integration to occur. Our study provides a plausible explanation for why sequence-conserved transposons, IS elements and integrons can be found disseminated among bacterial species. Moreover, natural transformation of integron harboring populations of competent bacteria revealed that interspecies exchange of gene cassettes can be highly efficient, and independent on genetic relatedness between donor and recipient. In conclusion, natural transformation provides a much broader capacity for horizontal acquisitions of genetic elements and hence, resistance traits from divergent species than previously assumed.
format	article
author	Sara Domingues Klaus Harms W Florian Fricke Pål J Johnsen Gabriela J da Silva Kaare Magne Nielsen
author_facet	Sara Domingues Klaus Harms W Florian Fricke Pål J Johnsen Gabriela J da Silva Kaare Magne Nielsen
author_sort	Sara Domingues
title	Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.
title_short	Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.
title_full	Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.
title_fullStr	Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.
title_full_unstemmed	Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.
title_sort	natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/92dcdc30f0994e77a5a1be035e52a244
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Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.

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