Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons.

<h4>Purpose</h4>To investigate the mechanism of the anti-epileptic effect of Ganoderma lucidum polysaccharides (GLP), the changes of intracellular calcium and CaMK II α expression in a model of epileptic neurons were investigated.<h4>Method</h4>Primary hippocampal neurons wer...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shu-Qiu Wang, Xiao-Jie Li, Hong-Bin Qiu, Zhi-Mei Jiang, Maria Simon, Xiao-Ru Ma, Lei Liu, Jun-Xing Liu, Fang-Fang Wang, Yan-Feng Liang, Jia-Mei Wu, Wei-Hua Di, Shaobo Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/92e1bc90a20f4150b703a00c91dbbc6c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:92e1bc90a20f4150b703a00c91dbbc6c
record_format	dspace
spelling	oai:doaj.org-article:92e1bc90a20f4150b703a00c91dbbc6c2021-11-25T06:08:57ZAnti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons.1932-620310.1371/journal.pone.0102161https://doaj.org/article/92e1bc90a20f4150b703a00c91dbbc6c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25010576/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Purpose</h4>To investigate the mechanism of the anti-epileptic effect of Ganoderma lucidum polysaccharides (GLP), the changes of intracellular calcium and CaMK II α expression in a model of epileptic neurons were investigated.<h4>Method</h4>Primary hippocampal neurons were divided into: 1) Control group, neurons were cultured with Neurobasal medium, for 3 hours; 2) Model group I: neurons were incubated with Mg(2+) free medium for 3 hours; 3) Model group II: neurons were incubated with Mg(2+) free medium for 3 hours then cultured with the normal medium for a further 3 hours; 4) GLP group I: neurons were incubated with Mg(2+) free medium containing GLP (0.375 mg/ml) for 3 hours; 5) GLP group II: neurons were incubated with Mg(2+) free medium for 3 hours then cultured with a normal culture medium containing GLP for a further 3 hours. The CaMK II α protein expression was assessed by Western-blot. Ca(2+) turnover in neurons was assessed using Fluo-3/AM which was added into the replacement medium and Ca(2+) turnover was observed under a laser scanning confocal microscope.<h4>Results</h4>The CaMK II α expression in the model groups was less than in the control groups, however, in the GLP groups, it was higher than that observed in the model group. Ca(2+) fluorescence intensity in GLP group I was significantly lower than that in model group I after 30 seconds, while in GLP group II, it was reduced significantly compared to model group II after 5 minutes.<h4>Conclusion</h4>GLP may inhibit calcium overload and promote CaMK II α expression to protect epileptic neurons.Shu-Qiu WangXiao-Jie LiHong-Bin QiuZhi-Mei JiangMaria SimonXiao-Ru MaLei LiuJun-Xing LiuFang-Fang WangYan-Feng LiangJia-Mei WuWei-Hua DiShaobo ZhouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102161 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shu-Qiu Wang Xiao-Jie Li Hong-Bin Qiu Zhi-Mei Jiang Maria Simon Xiao-Ru Ma Lei Liu Jun-Xing Liu Fang-Fang Wang Yan-Feng Liang Jia-Mei Wu Wei-Hua Di Shaobo Zhou Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons.
description	<h4>Purpose</h4>To investigate the mechanism of the anti-epileptic effect of Ganoderma lucidum polysaccharides (GLP), the changes of intracellular calcium and CaMK II α expression in a model of epileptic neurons were investigated.<h4>Method</h4>Primary hippocampal neurons were divided into: 1) Control group, neurons were cultured with Neurobasal medium, for 3 hours; 2) Model group I: neurons were incubated with Mg(2+) free medium for 3 hours; 3) Model group II: neurons were incubated with Mg(2+) free medium for 3 hours then cultured with the normal medium for a further 3 hours; 4) GLP group I: neurons were incubated with Mg(2+) free medium containing GLP (0.375 mg/ml) for 3 hours; 5) GLP group II: neurons were incubated with Mg(2+) free medium for 3 hours then cultured with a normal culture medium containing GLP for a further 3 hours. The CaMK II α protein expression was assessed by Western-blot. Ca(2+) turnover in neurons was assessed using Fluo-3/AM which was added into the replacement medium and Ca(2+) turnover was observed under a laser scanning confocal microscope.<h4>Results</h4>The CaMK II α expression in the model groups was less than in the control groups, however, in the GLP groups, it was higher than that observed in the model group. Ca(2+) fluorescence intensity in GLP group I was significantly lower than that in model group I after 30 seconds, while in GLP group II, it was reduced significantly compared to model group II after 5 minutes.<h4>Conclusion</h4>GLP may inhibit calcium overload and promote CaMK II α expression to protect epileptic neurons.
format	article
author	Shu-Qiu Wang Xiao-Jie Li Hong-Bin Qiu Zhi-Mei Jiang Maria Simon Xiao-Ru Ma Lei Liu Jun-Xing Liu Fang-Fang Wang Yan-Feng Liang Jia-Mei Wu Wei-Hua Di Shaobo Zhou
author_facet	Shu-Qiu Wang Xiao-Jie Li Hong-Bin Qiu Zhi-Mei Jiang Maria Simon Xiao-Ru Ma Lei Liu Jun-Xing Liu Fang-Fang Wang Yan-Feng Liang Jia-Mei Wu Wei-Hua Di Shaobo Zhou
author_sort	Shu-Qiu Wang
title	Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons.
title_short	Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons.
title_full	Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons.
title_fullStr	Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons.
title_full_unstemmed	Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons.
title_sort	anti-epileptic effect of ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of camkii α in epileptic hippocampal neurons.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/92e1bc90a20f4150b703a00c91dbbc6c
work_keys_str_mv	AT shuqiuwang antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT xiaojieli antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT hongbinqiu antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT zhimeijiang antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT mariasimon antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT xiaoruma antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT leiliu antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT junxingliu antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT fangfangwang antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT yanfengliang antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT jiameiwu antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT weihuadi antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons AT shaobozhou antiepilepticeffectofganodermalucidumpolysaccharidesbyinhibitionofintracellularcalciumaccumulationandstimulationofexpressionofcamkiiainepileptichippocampalneurons
_version_	1718414115694706688

Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons.

Ejemplares similares