Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry

Abstract Topical anesthetics are widely applied in order to relieve the discomfort and anxiety caused by needle insertion and other painful superficial interventions at the oral cavity. So far, there are no commercially available effective topical anesthetic formulations for that purpose, and the mo...

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Autores principales:	Lígia N. M. Ribeiro, Michelle Franz-Montan, Ana C. S. Alcântara, Márcia C. Breitkreitz, Simone R. Castro, Viviane A. Guilherme, Bruno V. Muniz, Gustavo H. Rodrigues da Silva, Eneida de Paula
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/92e308470eac4a48935a6247deb50cb1
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spelling	oai:doaj.org-article:92e308470eac4a48935a6247deb50cb12021-12-02T15:39:58ZHybrid nanofilms as topical anesthetics for pain-free procedures in dentistry10.1038/s41598-020-68247-02045-2322https://doaj.org/article/92e308470eac4a48935a6247deb50cb12020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68247-0https://doaj.org/toc/2045-2322Abstract Topical anesthetics are widely applied in order to relieve the discomfort and anxiety caused by needle insertion and other painful superficial interventions at the oral cavity. So far, there are no commercially available effective topical anesthetic formulations for that purpose, and the most of developments are related to hydrophilic and low mucoadhesive forms. Therefore, we have prepared different hybrid nanofilms composed of biopolymer matrices (chitosan, pectin, and chitosan-pectin) blended with nanostructured lipid carriers (NLC) loading the eutectic mixture of 5% lidocaine–prilocaine (LDC–PLC), in order to fulfill this gap in the market. These dual systems were processed as hybrid nanofilms by the solvent/casting method, and its mucoadhesive, structural and mechanical properties were detailed. The most appropriate hybrid nanofilm combined the advantages of both pectin (PCT) and NLC components. The resultant material presented sustained LDC–PLC release profile for more than 8 h; permeation across porcine buccal mucosa almost twice higher than control and non-cytotoxicity against 3T3 and HACAT cell lines. Then, the in vivo efficacy of PCT/NLC formulation was compared to biopolymer film and commercial drug, exhibiting the longest-lasting anesthetic effect (> 7 h), assessed by tail flick test in mice. These pectin-based hybrid nanofilms open perspectives for clinical trials and applications beyond Dentistry.Lígia N. M. RibeiroMichelle Franz-MontanAna C. S. AlcântaraMárcia C. BreitkreitzSimone R. CastroViviane A. GuilhermeBruno V. MunizGustavo H. Rodrigues da SilvaEneida de PaulaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lígia N. M. Ribeiro Michelle Franz-Montan Ana C. S. Alcântara Márcia C. Breitkreitz Simone R. Castro Viviane A. Guilherme Bruno V. Muniz Gustavo H. Rodrigues da Silva Eneida de Paula Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry
description	Abstract Topical anesthetics are widely applied in order to relieve the discomfort and anxiety caused by needle insertion and other painful superficial interventions at the oral cavity. So far, there are no commercially available effective topical anesthetic formulations for that purpose, and the most of developments are related to hydrophilic and low mucoadhesive forms. Therefore, we have prepared different hybrid nanofilms composed of biopolymer matrices (chitosan, pectin, and chitosan-pectin) blended with nanostructured lipid carriers (NLC) loading the eutectic mixture of 5% lidocaine–prilocaine (LDC–PLC), in order to fulfill this gap in the market. These dual systems were processed as hybrid nanofilms by the solvent/casting method, and its mucoadhesive, structural and mechanical properties were detailed. The most appropriate hybrid nanofilm combined the advantages of both pectin (PCT) and NLC components. The resultant material presented sustained LDC–PLC release profile for more than 8 h; permeation across porcine buccal mucosa almost twice higher than control and non-cytotoxicity against 3T3 and HACAT cell lines. Then, the in vivo efficacy of PCT/NLC formulation was compared to biopolymer film and commercial drug, exhibiting the longest-lasting anesthetic effect (> 7 h), assessed by tail flick test in mice. These pectin-based hybrid nanofilms open perspectives for clinical trials and applications beyond Dentistry.
format	article
author	Lígia N. M. Ribeiro Michelle Franz-Montan Ana C. S. Alcântara Márcia C. Breitkreitz Simone R. Castro Viviane A. Guilherme Bruno V. Muniz Gustavo H. Rodrigues da Silva Eneida de Paula
author_facet	Lígia N. M. Ribeiro Michelle Franz-Montan Ana C. S. Alcântara Márcia C. Breitkreitz Simone R. Castro Viviane A. Guilherme Bruno V. Muniz Gustavo H. Rodrigues da Silva Eneida de Paula
author_sort	Lígia N. M. Ribeiro
title	Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry
title_short	Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry
title_full	Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry
title_fullStr	Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry
title_full_unstemmed	Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry
title_sort	hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/92e308470eac4a48935a6247deb50cb1
work_keys_str_mv	AT ligianmribeiro hybridnanofilmsastopicalanestheticsforpainfreeproceduresindentistry AT michellefranzmontan hybridnanofilmsastopicalanestheticsforpainfreeproceduresindentistry AT anacsalcantara hybridnanofilmsastopicalanestheticsforpainfreeproceduresindentistry AT marciacbreitkreitz hybridnanofilmsastopicalanestheticsforpainfreeproceduresindentistry AT simonercastro hybridnanofilmsastopicalanestheticsforpainfreeproceduresindentistry AT vivianeaguilherme hybridnanofilmsastopicalanestheticsforpainfreeproceduresindentistry AT brunovmuniz hybridnanofilmsastopicalanestheticsforpainfreeproceduresindentistry AT gustavohrodriguesdasilva hybridnanofilmsastopicalanestheticsforpainfreeproceduresindentistry AT eneidadepaula hybridnanofilmsastopicalanestheticsforpainfreeproceduresindentistry
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Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry

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