The Effect of the Supernatant of Phytohemagglutinin (PHA) Stimulated Lymphocytes on the Isolated Rat Heart

BACKGROUND AND OBJECTIVE: Cytokines are mainly released during immune responses to antigens. In some circumstances such as toxic shock during super-antigen stimulation, high level of cytokines are released which it may have a dramatic impact on cardiovascular system. Therefore, in the present study...

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Autores principales: S Niazmand, N Zabihi, F Harandizadeh, AR Rezaee
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Publicado: Babol University of Medical Sciences 2011
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spelling oai:doaj.org-article:92e31b80a6de43f99113f3422da0cc772021-11-10T08:56:58ZThe Effect of the Supernatant of Phytohemagglutinin (PHA) Stimulated Lymphocytes on the Isolated Rat Heart1561-41072251-7170https://doaj.org/article/92e31b80a6de43f99113f3422da0cc772011-11-01T00:00:00Zhttp://jbums.org/article-1-3923-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Cytokines are mainly released during immune responses to antigens. In some circumstances such as toxic shock during super-antigen stimulation, high level of cytokines are released which it may have a dramatic impact on cardiovascular system. Therefore, in the present study the effects of supernatant of Phytohemaglotinin (PHA)- stimulated lymphocytes on isolated heart in rat have been evaluated.METHODS: This is an interventional study which was arranged on 20 male Wistar rats weighed 200-250g. The animals were randomly divided in two groups of control and test. The isolated heart in control group received the cell culture medium with PHA, while in test group the supernatants of PHA stimulated lymphocytes were introduced to isolated hearts. Three dilutions of supernatants (1/800, 1/400 and 1/200) on isolated rat hearts were used. Each concentration was infused to the heart for 10 minutes and the heart rate and the intraventricular pressure were recorded for 40 minutes. The peripheral blood mononuclear cells from healthy volunteers were isolated and stimulated by PHA in vitro for three days and then the supernatants were harvested. FINDINGS: The highest dilution, 1/800, reduced the heart rate 1 and 2 minute after infusion significantly (p<0.05). This effect was significant 10 minutes after infusion for 1/400 and 1/200 dilutions as well. However, the effect of supernatant on the Max dp/dt, Min dp/dt, Max LV and Min LV were not significant.CONCLUSION: In general the data in the present study shows that cytokine pattern of PHA stimulated lymphocyte had a marked negative impact on heart rate which might be due to the effect of these cytokines on stimulatory and conductivity (chronotropy) of cardiac muscle. On the other hand, there was not any significant effect on contractility.S NiazmandN ZabihiF Harandizadeh,AR RezaeeBabol University of Medical Sciencesarticleisolated heartheart contractilityheart ratephytohemagglutininlymphocyteMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 13, Iss 6, Pp 37-44 (2011)
institution DOAJ
collection DOAJ
language EN
FA
topic isolated heart
heart contractility
heart rate
phytohemagglutinin
lymphocyte
Medicine
R
Medicine (General)
R5-920
spellingShingle isolated heart
heart contractility
heart rate
phytohemagglutinin
lymphocyte
Medicine
R
Medicine (General)
R5-920
S Niazmand
N Zabihi
F Harandizadeh,
AR Rezaee
The Effect of the Supernatant of Phytohemagglutinin (PHA) Stimulated Lymphocytes on the Isolated Rat Heart
description BACKGROUND AND OBJECTIVE: Cytokines are mainly released during immune responses to antigens. In some circumstances such as toxic shock during super-antigen stimulation, high level of cytokines are released which it may have a dramatic impact on cardiovascular system. Therefore, in the present study the effects of supernatant of Phytohemaglotinin (PHA)- stimulated lymphocytes on isolated heart in rat have been evaluated.METHODS: This is an interventional study which was arranged on 20 male Wistar rats weighed 200-250g. The animals were randomly divided in two groups of control and test. The isolated heart in control group received the cell culture medium with PHA, while in test group the supernatants of PHA stimulated lymphocytes were introduced to isolated hearts. Three dilutions of supernatants (1/800, 1/400 and 1/200) on isolated rat hearts were used. Each concentration was infused to the heart for 10 minutes and the heart rate and the intraventricular pressure were recorded for 40 minutes. The peripheral blood mononuclear cells from healthy volunteers were isolated and stimulated by PHA in vitro for three days and then the supernatants were harvested. FINDINGS: The highest dilution, 1/800, reduced the heart rate 1 and 2 minute after infusion significantly (p<0.05). This effect was significant 10 minutes after infusion for 1/400 and 1/200 dilutions as well. However, the effect of supernatant on the Max dp/dt, Min dp/dt, Max LV and Min LV were not significant.CONCLUSION: In general the data in the present study shows that cytokine pattern of PHA stimulated lymphocyte had a marked negative impact on heart rate which might be due to the effect of these cytokines on stimulatory and conductivity (chronotropy) of cardiac muscle. On the other hand, there was not any significant effect on contractility.
format article
author S Niazmand
N Zabihi
F Harandizadeh,
AR Rezaee
author_facet S Niazmand
N Zabihi
F Harandizadeh,
AR Rezaee
author_sort S Niazmand
title The Effect of the Supernatant of Phytohemagglutinin (PHA) Stimulated Lymphocytes on the Isolated Rat Heart
title_short The Effect of the Supernatant of Phytohemagglutinin (PHA) Stimulated Lymphocytes on the Isolated Rat Heart
title_full The Effect of the Supernatant of Phytohemagglutinin (PHA) Stimulated Lymphocytes on the Isolated Rat Heart
title_fullStr The Effect of the Supernatant of Phytohemagglutinin (PHA) Stimulated Lymphocytes on the Isolated Rat Heart
title_full_unstemmed The Effect of the Supernatant of Phytohemagglutinin (PHA) Stimulated Lymphocytes on the Isolated Rat Heart
title_sort effect of the supernatant of phytohemagglutinin (pha) stimulated lymphocytes on the isolated rat heart
publisher Babol University of Medical Sciences
publishDate 2011
url https://doaj.org/article/92e31b80a6de43f99113f3422da0cc77
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