Hadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, <named-content content-type="genus-species">Fusarium oxysporum</named-content>

ABSTRACT The search for viruses infecting fungi, or mycoviruses, has extended our knowledge about the diversity of RNA viruses, as exemplified by the discovery of polymycoviruses, a phylogenetic group of multisegmented RNA viruses with unusual forms. The genomic RNAs of known polymycoviruses, which...

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Autores principales:	Yukiyo Sato, Wajeeha Shamsi, Atif Jamal, Muhammad Faraz Bhatti, Hideki Kondo, Nobuhiro Suzuki
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	fungal virus polymycovirus Fusarium oxysporum multisegmented RNA virus capsidless Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/92f51edccd0340c3849833f67e37fc85
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spelling	oai:doaj.org-article:92f51edccd0340c3849833f67e37fc852021-11-15T15:56:47ZHadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, <named-content content-type="genus-species">Fusarium oxysporum</named-content>10.1128/mBio.00450-202150-7511https://doaj.org/article/92f51edccd0340c3849833f67e37fc852020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00450-20https://doaj.org/toc/2150-7511ABSTRACT The search for viruses infecting fungi, or mycoviruses, has extended our knowledge about the diversity of RNA viruses, as exemplified by the discovery of polymycoviruses, a phylogenetic group of multisegmented RNA viruses with unusual forms. The genomic RNAs of known polymycoviruses, which show a phylogenetic affinity for animal positive-sense single-stranded RNA [(+)RNA] viruses such as caliciviruses, are comprised of four conserved segments with an additional zero to four segments. The double-stranded form of polymycovirus genomic RNA is assumed to be associated with a virally encoded protein (proline-alanine-serine-rich protein [PASrp]) in either of two manners: a capsidless colloidal form or a filamentous encapsidated form. Detailed molecular characterizations of polymycoviruses, however, have been conducted for only a few strains. Here, a novel polymyco-related virus named Hadaka virus 1 (HadV1), from the phytopathogenic fungus Fusarium oxysporum, was characterized. The genomic RNA of HadV1 consisted of an 11-segmented positive-sense RNA with highly conserved terminal nucleotide sequences. HadV1 shared the three conserved segments with known polymycoviruses but lacked the PASrp-encoding segment. Unlike the known polymycoviruses and encapsidated viruses, HadV1 was not pelleted by conventional ultracentrifugation, possibly due to the lack of PASrp. This result implied that HadV1 exists only as a soluble form with naked RNA. Nevertheless, the 11 genomic segments of HadV1 have been stably maintained through host subculturing and conidiation. Taken together, the results of this study revealed a virus with a potential novel virus lifestyle, carrying many genomic segments without typical capsids or PASrp-associated forms. IMPORTANCE Fungi collectively host various RNA viruses. Examples include encapsidated double-stranded RNA (dsRNA) viruses with diverse numbers of genomic segments (from 1 to 12) and capsidless viruses with nonsegmented (+)RNA genomes. Recently, viruses with unusual intermediate features of an infectious entity between encapsidated dsRNA viruses and capsidless (+)RNA viruses were found. They are called polymycoviruses, which typically have four to eight dsRNA genomic segments associated with one of the virus-encoded proteins and are phylogenetically distantly related to animal (+)RNA caliciviruses. Here, we identified a novel virus phylogenetically related to polymycoviruses, from the phytopathogenic fungus Fusarium oxysporum. The virus, termed Hadaka virus 1 (HadV1), has 11 (+)RNA genomic segments, the largest number in known (+)RNA viruses. Nevertheless, HadV1 lacked a typical structural protein of polymycoviruses and was not pelleted by standard ultracentrifugation, implying an unusual capsidless nature of HadV1. This study reveals a potential novel lifestyle of multisegmented RNA viruses.Yukiyo SatoWajeeha ShamsiAtif JamalMuhammad Faraz BhattiHideki KondoNobuhiro SuzukiAmerican Society for Microbiologyarticlefungal viruspolymycovirusFusarium oxysporummultisegmentedRNA viruscapsidlessMicrobiologyQR1-502ENmBio, Vol 11, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	fungal virus polymycovirus Fusarium oxysporum multisegmented RNA virus capsidless Microbiology QR1-502
spellingShingle	fungal virus polymycovirus Fusarium oxysporum multisegmented RNA virus capsidless Microbiology QR1-502 Yukiyo Sato Wajeeha Shamsi Atif Jamal Muhammad Faraz Bhatti Hideki Kondo Nobuhiro Suzuki Hadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, <named-content content-type="genus-species">Fusarium oxysporum</named-content>
description	ABSTRACT The search for viruses infecting fungi, or mycoviruses, has extended our knowledge about the diversity of RNA viruses, as exemplified by the discovery of polymycoviruses, a phylogenetic group of multisegmented RNA viruses with unusual forms. The genomic RNAs of known polymycoviruses, which show a phylogenetic affinity for animal positive-sense single-stranded RNA [(+)RNA] viruses such as caliciviruses, are comprised of four conserved segments with an additional zero to four segments. The double-stranded form of polymycovirus genomic RNA is assumed to be associated with a virally encoded protein (proline-alanine-serine-rich protein [PASrp]) in either of two manners: a capsidless colloidal form or a filamentous encapsidated form. Detailed molecular characterizations of polymycoviruses, however, have been conducted for only a few strains. Here, a novel polymyco-related virus named Hadaka virus 1 (HadV1), from the phytopathogenic fungus Fusarium oxysporum, was characterized. The genomic RNA of HadV1 consisted of an 11-segmented positive-sense RNA with highly conserved terminal nucleotide sequences. HadV1 shared the three conserved segments with known polymycoviruses but lacked the PASrp-encoding segment. Unlike the known polymycoviruses and encapsidated viruses, HadV1 was not pelleted by conventional ultracentrifugation, possibly due to the lack of PASrp. This result implied that HadV1 exists only as a soluble form with naked RNA. Nevertheless, the 11 genomic segments of HadV1 have been stably maintained through host subculturing and conidiation. Taken together, the results of this study revealed a virus with a potential novel virus lifestyle, carrying many genomic segments without typical capsids or PASrp-associated forms. IMPORTANCE Fungi collectively host various RNA viruses. Examples include encapsidated double-stranded RNA (dsRNA) viruses with diverse numbers of genomic segments (from 1 to 12) and capsidless viruses with nonsegmented (+)RNA genomes. Recently, viruses with unusual intermediate features of an infectious entity between encapsidated dsRNA viruses and capsidless (+)RNA viruses were found. They are called polymycoviruses, which typically have four to eight dsRNA genomic segments associated with one of the virus-encoded proteins and are phylogenetically distantly related to animal (+)RNA caliciviruses. Here, we identified a novel virus phylogenetically related to polymycoviruses, from the phytopathogenic fungus Fusarium oxysporum. The virus, termed Hadaka virus 1 (HadV1), has 11 (+)RNA genomic segments, the largest number in known (+)RNA viruses. Nevertheless, HadV1 lacked a typical structural protein of polymycoviruses and was not pelleted by standard ultracentrifugation, implying an unusual capsidless nature of HadV1. This study reveals a potential novel lifestyle of multisegmented RNA viruses.
format	article
author	Yukiyo Sato Wajeeha Shamsi Atif Jamal Muhammad Faraz Bhatti Hideki Kondo Nobuhiro Suzuki
author_facet	Yukiyo Sato Wajeeha Shamsi Atif Jamal Muhammad Faraz Bhatti Hideki Kondo Nobuhiro Suzuki
author_sort	Yukiyo Sato
title	Hadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, <named-content content-type="genus-species">Fusarium oxysporum</named-content>
title_short	Hadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, <named-content content-type="genus-species">Fusarium oxysporum</named-content>
title_full	Hadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, <named-content content-type="genus-species">Fusarium oxysporum</named-content>
title_fullStr	Hadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, <named-content content-type="genus-species">Fusarium oxysporum</named-content>
title_full_unstemmed	Hadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, <named-content content-type="genus-species">Fusarium oxysporum</named-content>
title_sort	hadaka virus 1: a capsidless eleven-segmented positive-sense single-stranded rna virus from a phytopathogenic fungus, <named-content content-type="genus-species">fusarium oxysporum</named-content>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/92f51edccd0340c3849833f67e37fc85
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Hadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, <named-content content-type="genus-species">Fusarium oxysporum</named-content>

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