Phenotypic Evolution of Therapeutic <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium after Invasion of TRAMP Mouse Prostate Tumor

ABSTRACT Salmonella has been of interest in cancer research due to its intrinsic ability to selectively target and colonize within tumors, leading to tumor cell death. Current research indicates promising use of Salmonella in regular administrations to remove tumors in mouse models while minimizing...

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Autores principales:	Elizabeth Choe, Robert A. Kazmierczak, Abraham Eisenstark
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/92f90f1585fa44ca8d824e93bcaf28a0
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spelling	oai:doaj.org-article:92f90f1585fa44ca8d824e93bcaf28a02021-11-15T15:47:21ZPhenotypic Evolution of Therapeutic <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium after Invasion of TRAMP Mouse Prostate Tumor10.1128/mBio.01182-142150-7511https://doaj.org/article/92f90f1585fa44ca8d824e93bcaf28a02014-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01182-14https://doaj.org/toc/2150-7511ABSTRACT Salmonella has been of interest in cancer research due to its intrinsic ability to selectively target and colonize within tumors, leading to tumor cell death. Current research indicates promising use of Salmonella in regular administrations to remove tumors in mouse models while minimizing toxic side effects. However, selection of mutants during such long-term tumor colonization is a safety concern, and understanding selection of certain phenotypes within a tumor is an important consideration in predicting the long-term success of bacterium-based cancer treatment strategies. Thus, we have made an initial examination of selected phenotypes in a therapeutic Salmonella enterica serovar Typhimurium population developed from an archival wild-type LT2 strain and intraperitoneally injected into a 6-month-old TRAMP (transgenic adenocarcinoma of mouse prostate) mouse. We compared the original injected strain to isolates recovered from prostate tumors and those recovered from the spleen and liver of non-tumor-bearing TRAMP mice through phenotypic assessments of bacteriophage susceptibility, motility, growth rates, morphology, and metabolic activity. Tumor isolate traits, particularly the loss of wild-type motility and flagella, reflect the selective pressure of the tumor, while the maintenance of bacteriophage resistance indicates no active selection to remove this robust trait. We posit that the Salmonella population adopts certain strategies to minimize energy consumption and maximize survival and proliferation once within the tumor. We find these insights to be nonnegligible considerations in the development of cancer therapies involving bacteria and suggest further examinations into the evolution of therapeutic strains during passage through tumors. IMPORTANCE Salmonella is of interest in cancer research due to its intrinsic abilities to selectively target, colonize, and replicate within tumors, leading to tumor cell death. However, mutation of strains during long-term colonization within tumors is a safety concern, and understanding their evolution within a tumor is an important consideration in predicting the long-term success of bacterium-based cancer treatment strategies. Thus, we have made an initial examination of phenotypically diverse Salmonella colonies recovered from a therapeutic Salmonella strain that we developed and injected into prostate tumor-bearing mice. We compared the bacteriophage susceptibility, motility, growth rates, morphology, and metabolic activity of the original therapeutic strain to those of strains recovered from prostate tumors of tumor-bearing mice and the liver and spleen of non-tumor-bearing mice. Our results suggest that the Salmonella population adopts certain strategies to minimize energy consumption and maximize survival and proliferation once within the tumor, leading to phenotypic changes in the strain.Elizabeth ChoeRobert A. KazmierczakAbraham EisenstarkAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 4 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Elizabeth Choe Robert A. Kazmierczak Abraham Eisenstark Phenotypic Evolution of Therapeutic <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium after Invasion of TRAMP Mouse Prostate Tumor
description	ABSTRACT Salmonella has been of interest in cancer research due to its intrinsic ability to selectively target and colonize within tumors, leading to tumor cell death. Current research indicates promising use of Salmonella in regular administrations to remove tumors in mouse models while minimizing toxic side effects. However, selection of mutants during such long-term tumor colonization is a safety concern, and understanding selection of certain phenotypes within a tumor is an important consideration in predicting the long-term success of bacterium-based cancer treatment strategies. Thus, we have made an initial examination of selected phenotypes in a therapeutic Salmonella enterica serovar Typhimurium population developed from an archival wild-type LT2 strain and intraperitoneally injected into a 6-month-old TRAMP (transgenic adenocarcinoma of mouse prostate) mouse. We compared the original injected strain to isolates recovered from prostate tumors and those recovered from the spleen and liver of non-tumor-bearing TRAMP mice through phenotypic assessments of bacteriophage susceptibility, motility, growth rates, morphology, and metabolic activity. Tumor isolate traits, particularly the loss of wild-type motility and flagella, reflect the selective pressure of the tumor, while the maintenance of bacteriophage resistance indicates no active selection to remove this robust trait. We posit that the Salmonella population adopts certain strategies to minimize energy consumption and maximize survival and proliferation once within the tumor. We find these insights to be nonnegligible considerations in the development of cancer therapies involving bacteria and suggest further examinations into the evolution of therapeutic strains during passage through tumors. IMPORTANCE Salmonella is of interest in cancer research due to its intrinsic abilities to selectively target, colonize, and replicate within tumors, leading to tumor cell death. However, mutation of strains during long-term colonization within tumors is a safety concern, and understanding their evolution within a tumor is an important consideration in predicting the long-term success of bacterium-based cancer treatment strategies. Thus, we have made an initial examination of phenotypically diverse Salmonella colonies recovered from a therapeutic Salmonella strain that we developed and injected into prostate tumor-bearing mice. We compared the bacteriophage susceptibility, motility, growth rates, morphology, and metabolic activity of the original therapeutic strain to those of strains recovered from prostate tumors of tumor-bearing mice and the liver and spleen of non-tumor-bearing mice. Our results suggest that the Salmonella population adopts certain strategies to minimize energy consumption and maximize survival and proliferation once within the tumor, leading to phenotypic changes in the strain.
format	article
author	Elizabeth Choe Robert A. Kazmierczak Abraham Eisenstark
author_facet	Elizabeth Choe Robert A. Kazmierczak Abraham Eisenstark
author_sort	Elizabeth Choe
title	Phenotypic Evolution of Therapeutic <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium after Invasion of TRAMP Mouse Prostate Tumor
title_short	Phenotypic Evolution of Therapeutic <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium after Invasion of TRAMP Mouse Prostate Tumor
title_full	Phenotypic Evolution of Therapeutic <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium after Invasion of TRAMP Mouse Prostate Tumor
title_fullStr	Phenotypic Evolution of Therapeutic <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium after Invasion of TRAMP Mouse Prostate Tumor
title_full_unstemmed	Phenotypic Evolution of Therapeutic <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium after Invasion of TRAMP Mouse Prostate Tumor
title_sort	phenotypic evolution of therapeutic <named-content content-type="genus-species">salmonella enterica</named-content> serovar typhimurium after invasion of tramp mouse prostate tumor
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/92f90f1585fa44ca8d824e93bcaf28a0
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Phenotypic Evolution of Therapeutic <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium after Invasion of TRAMP Mouse Prostate Tumor

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