Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry

Cross-linking mass spectrometry (MS) is an important tool in structural biology, but its application to protein-DNA complexes has been hampered by low cross-linking efficiency. Here, the authors develop a femtosecond UV-laser induced cross-linking MS workflow to map protein-DNA interactions in vitro...

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Autores principales:	Alexander Reim, Roland Ackermann, Jofre Font-Mateu, Robert Kammel, Miguel Beato, Stefan Nolte, Matthias Mann, Christoph Russmann, Michael Wierer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/92fea555226e4693b60d8da44e4a57ca
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spelling	oai:doaj.org-article:92fea555226e4693b60d8da44e4a57ca2021-12-02T16:04:17ZAtomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry10.1038/s41467-020-16837-x2041-1723https://doaj.org/article/92fea555226e4693b60d8da44e4a57ca2020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16837-xhttps://doaj.org/toc/2041-1723Cross-linking mass spectrometry (MS) is an important tool in structural biology, but its application to protein-DNA complexes has been hampered by low cross-linking efficiency. Here, the authors develop a femtosecond UV-laser induced cross-linking MS workflow to map protein-DNA interactions in vitro and in cells.Alexander ReimRoland AckermannJofre Font-MateuRobert KammelMiguel BeatoStefan NolteMatthias MannChristoph RussmannMichael WiererNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alexander Reim Roland Ackermann Jofre Font-Mateu Robert Kammel Miguel Beato Stefan Nolte Matthias Mann Christoph Russmann Michael Wierer Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry
description	Cross-linking mass spectrometry (MS) is an important tool in structural biology, but its application to protein-DNA complexes has been hampered by low cross-linking efficiency. Here, the authors develop a femtosecond UV-laser induced cross-linking MS workflow to map protein-DNA interactions in vitro and in cells.
format	article
author	Alexander Reim Roland Ackermann Jofre Font-Mateu Robert Kammel Miguel Beato Stefan Nolte Matthias Mann Christoph Russmann Michael Wierer
author_facet	Alexander Reim Roland Ackermann Jofre Font-Mateu Robert Kammel Miguel Beato Stefan Nolte Matthias Mann Christoph Russmann Michael Wierer
author_sort	Alexander Reim
title	Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry
title_short	Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry
title_full	Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry
title_fullStr	Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry
title_full_unstemmed	Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry
title_sort	atomic-resolution mapping of transcription factor-dna interactions by femtosecond laser crosslinking and mass spectrometry
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/92fea555226e4693b60d8da44e4a57ca
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Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry

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