Microbiological Contamination of the Office Environment in Dental and Medical Practice

The microbiological contamination of the environment in independent healthcare facilities such as dental and general practitioner offices was poorly studied. The aims of this study were to describe qualitatively and quantitatively the bacterial and fungal contamination in these healthcare facilities...

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Autores principales:	Alexandre Baudet, Monique Guillaso, Léonie Grimmer, MEDIQAI Study Group, Marie Regad, Arnaud Florentin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	environmental microbiology environmental contamination indoor air dental offices general practitioner offices antibiotic resistance Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/930440c6824348598808da76c766d56c
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spelling	oai:doaj.org-article:930440c6824348598808da76c766d56c2021-11-25T16:24:14ZMicrobiological Contamination of the Office Environment in Dental and Medical Practice10.3390/antibiotics101113752079-6382https://doaj.org/article/930440c6824348598808da76c766d56c2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1375https://doaj.org/toc/2079-6382The microbiological contamination of the environment in independent healthcare facilities such as dental and general practitioner offices was poorly studied. The aims of this study were to describe qualitatively and quantitatively the bacterial and fungal contamination in these healthcare facilities and to analyze the antibiotic resistance of bacterial pathogens identified. Microbiological samples were taken from the surfaces of waiting, consulting, and sterilization rooms and from the air of waiting room of ten dental and general practitioner offices. Six surface samples were collected in each sampled room using agar contact plates and swabs. Indoor air samples were collected in waiting rooms using a single-stage impactor. Bacteria and fungi were cultured, then counted and identified. Antibiograms were performed to test the antibiotic susceptibility of bacterial pathogens. On the surfaces, median concentrations of bacteria and fungi were 126 (range: 0–1280) and 26 (range: 0–188) CFU/100 cm<sup>2</sup>, respectively. In indoor air, those concentrations were 403 (range: 118–732) and 327 (range: 32–806) CFU/m<sup>3</sup>, respectively. The main micro-organisms identified were Gram-positive cocci and filamentous fungi, including six ubiquitous genera: <i>Micrococcus</i>, <i>Staphylococcus</i>, <i>Cladosporium</i>, <i>Penicillium</i>, <i>Aspergillus</i>, and <i>Alternaria</i>. Some antibiotic-resistant bacteria were identified in general practitioner offices (penicillin- and erythromycin-resistant <i>Staphylococcus aureus</i>), but none in dental offices. The dental and general practitioner offices present a poor microbiological contamination with rare pathogenic micro-organisms.Alexandre BaudetMonique GuillasoLéonie GrimmerMEDIQAI Study GroupMarie RegadArnaud FlorentinMDPI AGarticleenvironmental microbiologyenvironmental contaminationindoor airdental officesgeneral practitioner officesantibiotic resistanceTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1375, p 1375 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	environmental microbiology environmental contamination indoor air dental offices general practitioner offices antibiotic resistance Therapeutics. Pharmacology RM1-950
spellingShingle	environmental microbiology environmental contamination indoor air dental offices general practitioner offices antibiotic resistance Therapeutics. Pharmacology RM1-950 Alexandre Baudet Monique Guillaso Léonie Grimmer MEDIQAI Study Group Marie Regad Arnaud Florentin Microbiological Contamination of the Office Environment in Dental and Medical Practice
description	The microbiological contamination of the environment in independent healthcare facilities such as dental and general practitioner offices was poorly studied. The aims of this study were to describe qualitatively and quantitatively the bacterial and fungal contamination in these healthcare facilities and to analyze the antibiotic resistance of bacterial pathogens identified. Microbiological samples were taken from the surfaces of waiting, consulting, and sterilization rooms and from the air of waiting room of ten dental and general practitioner offices. Six surface samples were collected in each sampled room using agar contact plates and swabs. Indoor air samples were collected in waiting rooms using a single-stage impactor. Bacteria and fungi were cultured, then counted and identified. Antibiograms were performed to test the antibiotic susceptibility of bacterial pathogens. On the surfaces, median concentrations of bacteria and fungi were 126 (range: 0–1280) and 26 (range: 0–188) CFU/100 cm<sup>2</sup>, respectively. In indoor air, those concentrations were 403 (range: 118–732) and 327 (range: 32–806) CFU/m<sup>3</sup>, respectively. The main micro-organisms identified were Gram-positive cocci and filamentous fungi, including six ubiquitous genera: <i>Micrococcus</i>, <i>Staphylococcus</i>, <i>Cladosporium</i>, <i>Penicillium</i>, <i>Aspergillus</i>, and <i>Alternaria</i>. Some antibiotic-resistant bacteria were identified in general practitioner offices (penicillin- and erythromycin-resistant <i>Staphylococcus aureus</i>), but none in dental offices. The dental and general practitioner offices present a poor microbiological contamination with rare pathogenic micro-organisms.
format	article
author	Alexandre Baudet Monique Guillaso Léonie Grimmer MEDIQAI Study Group Marie Regad Arnaud Florentin
author_facet	Alexandre Baudet Monique Guillaso Léonie Grimmer MEDIQAI Study Group Marie Regad Arnaud Florentin
author_sort	Alexandre Baudet
title	Microbiological Contamination of the Office Environment in Dental and Medical Practice
title_short	Microbiological Contamination of the Office Environment in Dental and Medical Practice
title_full	Microbiological Contamination of the Office Environment in Dental and Medical Practice
title_fullStr	Microbiological Contamination of the Office Environment in Dental and Medical Practice
title_full_unstemmed	Microbiological Contamination of the Office Environment in Dental and Medical Practice
title_sort	microbiological contamination of the office environment in dental and medical practice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/930440c6824348598808da76c766d56c
work_keys_str_mv	AT alexandrebaudet microbiologicalcontaminationoftheofficeenvironmentindentalandmedicalpractice AT moniqueguillaso microbiologicalcontaminationoftheofficeenvironmentindentalandmedicalpractice AT leoniegrimmer microbiologicalcontaminationoftheofficeenvironmentindentalandmedicalpractice AT mediqaistudygroup microbiologicalcontaminationoftheofficeenvironmentindentalandmedicalpractice AT marieregad microbiologicalcontaminationoftheofficeenvironmentindentalandmedicalpractice AT arnaudflorentin microbiologicalcontaminationoftheofficeenvironmentindentalandmedicalpractice
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Microbiological Contamination of the Office Environment in Dental and Medical Practice

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