An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein

Abstract Protein splicing is a post-translational process by which an intein catalyzes its own excision from flanking polypeptides, or exteins, concomitant with extein ligation. Many inteins have nested homing endonuclease domains that facilitate their propagation into intein-less alleles, whereas o...

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Autores principales:	Jennie E. Williams, Mario V. Jaramillo, Zhong Li, Jing Zhao, Chunyu Wang, Hongmin Li, Kenneth V. Mills
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9304cb1752b14637aaaec911becdd49b
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spelling	oai:doaj.org-article:9304cb1752b14637aaaec911becdd49b2021-12-02T18:25:04ZAn alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein10.1038/s41598-021-91090-w2045-2322https://doaj.org/article/9304cb1752b14637aaaec911becdd49b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91090-whttps://doaj.org/toc/2045-2322Abstract Protein splicing is a post-translational process by which an intein catalyzes its own excision from flanking polypeptides, or exteins, concomitant with extein ligation. Many inteins have nested homing endonuclease domains that facilitate their propagation into intein-less alleles, whereas other inteins lack the homing endonuclease (HEN) and are called mini-inteins. The mini-intein that interrupts the DNA PolII of Pyrococcus horikoshii has a linker region in place of the HEN domain that is shorter than the linker in a closely related intein from Pyrococcus abyssi. The P. horikoshii PolII intein requires a higher temperature for catalytic activity and is more stable to digestion by the thermostable protease thermolysin, suggesting that it is more rigid than the P. abyssi intein. We solved a crystal structure of the intein precursor that revealed a domain-swapped dimer. Inteins found as domain swapped dimers have been shown to promote intein-mediated protein alternative splicing, but the solved P. horikoshii PolII intein structure has an active site unlikely to be catalytically competent.Jennie E. WilliamsMario V. JaramilloZhong LiJing ZhaoChunyu WangHongmin LiKenneth V. MillsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennie E. Williams Mario V. Jaramillo Zhong Li Jing Zhao Chunyu Wang Hongmin Li Kenneth V. Mills An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein
description	Abstract Protein splicing is a post-translational process by which an intein catalyzes its own excision from flanking polypeptides, or exteins, concomitant with extein ligation. Many inteins have nested homing endonuclease domains that facilitate their propagation into intein-less alleles, whereas other inteins lack the homing endonuclease (HEN) and are called mini-inteins. The mini-intein that interrupts the DNA PolII of Pyrococcus horikoshii has a linker region in place of the HEN domain that is shorter than the linker in a closely related intein from Pyrococcus abyssi. The P. horikoshii PolII intein requires a higher temperature for catalytic activity and is more stable to digestion by the thermostable protease thermolysin, suggesting that it is more rigid than the P. abyssi intein. We solved a crystal structure of the intein precursor that revealed a domain-swapped dimer. Inteins found as domain swapped dimers have been shown to promote intein-mediated protein alternative splicing, but the solved P. horikoshii PolII intein structure has an active site unlikely to be catalytically competent.
format	article
author	Jennie E. Williams Mario V. Jaramillo Zhong Li Jing Zhao Chunyu Wang Hongmin Li Kenneth V. Mills
author_facet	Jennie E. Williams Mario V. Jaramillo Zhong Li Jing Zhao Chunyu Wang Hongmin Li Kenneth V. Mills
author_sort	Jennie E. Williams
title	An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein
title_short	An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein
title_full	An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein
title_fullStr	An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein
title_full_unstemmed	An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein
title_sort	alternative domain-swapped structure of the pyrococcus horikoshii polii mini-intein
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9304cb1752b14637aaaec911becdd49b
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An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein

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