MiRNA-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin

Abstract Diabetic nephropathy is one of the most prevalent chronic complications of Diabetes mellitus, but its pathogenesis remains elusive. This study was designed to determine the role of tristetraprolin (TTP), inflammatory cytokines and microRNAs (miRNAs) in DN. The blood and urine samples were o...

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Autores principales:	Jia Guo, Jing Li, Jing Zhao, Shuguang Yang, Luyao Wang, Genyang Cheng, Dong Liu, Jing Xiao, Zhangsuo Liu, Zhanzheng Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9305eebe24aa4598a0e2461ceefc447b
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spelling	oai:doaj.org-article:9305eebe24aa4598a0e2461ceefc447b2021-12-02T12:31:59ZMiRNA-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin10.1038/s41598-017-01027-52045-2322https://doaj.org/article/9305eebe24aa4598a0e2461ceefc447b2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01027-5https://doaj.org/toc/2045-2322Abstract Diabetic nephropathy is one of the most prevalent chronic complications of Diabetes mellitus, but its pathogenesis remains elusive. This study was designed to determine the role of tristetraprolin (TTP), inflammatory cytokines and microRNAs (miRNAs) in DN. The blood and urine samples were obtained from 32 patients with DN, 33 patients with type 2 DM, and 35 normal healthy subjects as controls. Renal tissue samples were also obtained from 10 DN patients and 10 normal controls. The miRNA microarray analyses were performed in pooled plasma and urine sediment samples of eight DN patients and eight age- and sex-matched health control subjects and three paired renal tissues from patients with DN and normal controls. Conditionally immortalized mouse podocytes (MPC5) were used a cell model. The expressions of TTP and cytokines in patient samples and cultured cells were determined by qRT-PCR and Western blotting or ELISA. Our results indicated that miRNA-29c directly targeted TTP and promoted inflammatory response under hyperglycemic conditions. Overexpression of miRNA-29c in podocytes resulted in an increase in inflammatory cytokines and inhibition of miRNA-29c by using its inhibitor reduced the inflammatory cytokines in podocytes. Finally, miRNA-29c promoted the progression of DN by targeting TTP, providing a target for a therapeutic intervention of DN.Jia GuoJing LiJing ZhaoShuguang YangLuyao WangGenyang ChengDong LiuJing XiaoZhangsuo LiuZhanzheng ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jia Guo Jing Li Jing Zhao Shuguang Yang Luyao Wang Genyang Cheng Dong Liu Jing Xiao Zhangsuo Liu Zhanzheng Zhao MiRNA-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin
description	Abstract Diabetic nephropathy is one of the most prevalent chronic complications of Diabetes mellitus, but its pathogenesis remains elusive. This study was designed to determine the role of tristetraprolin (TTP), inflammatory cytokines and microRNAs (miRNAs) in DN. The blood and urine samples were obtained from 32 patients with DN, 33 patients with type 2 DM, and 35 normal healthy subjects as controls. Renal tissue samples were also obtained from 10 DN patients and 10 normal controls. The miRNA microarray analyses were performed in pooled plasma and urine sediment samples of eight DN patients and eight age- and sex-matched health control subjects and three paired renal tissues from patients with DN and normal controls. Conditionally immortalized mouse podocytes (MPC5) were used a cell model. The expressions of TTP and cytokines in patient samples and cultured cells were determined by qRT-PCR and Western blotting or ELISA. Our results indicated that miRNA-29c directly targeted TTP and promoted inflammatory response under hyperglycemic conditions. Overexpression of miRNA-29c in podocytes resulted in an increase in inflammatory cytokines and inhibition of miRNA-29c by using its inhibitor reduced the inflammatory cytokines in podocytes. Finally, miRNA-29c promoted the progression of DN by targeting TTP, providing a target for a therapeutic intervention of DN.
format	article
author	Jia Guo Jing Li Jing Zhao Shuguang Yang Luyao Wang Genyang Cheng Dong Liu Jing Xiao Zhangsuo Liu Zhanzheng Zhao
author_facet	Jia Guo Jing Li Jing Zhao Shuguang Yang Luyao Wang Genyang Cheng Dong Liu Jing Xiao Zhangsuo Liu Zhanzheng Zhao
author_sort	Jia Guo
title	MiRNA-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin
title_short	MiRNA-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin
title_full	MiRNA-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin
title_fullStr	MiRNA-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin
title_full_unstemmed	MiRNA-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin
title_sort	mirna-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9305eebe24aa4598a0e2461ceefc447b
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MiRNA-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin

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