An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera

Polycythemia Vera (PV) is a myeloproliferative neoplasm with increased risk of thrombosis and progression to myelofibrosis. Chronic inflammation is commonly observed in myeloproliferative neoplasms including PV. The inflammatory network includes the extracellular vesicles (EVs), which play a role in...

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Autores principales:	Monica Barone, Martina Barone, Francesca Ricci, Giuseppe Auteri, Giulia Corradi, Francesco Fabbri, Valentina Papa, Erika Bandini, Giovanna Cenacchi, Pier Luigi Tazzari, Nicola Vianelli, Silvia Turroni, Michele Cavo, Francesca Palandri, Marco Candela, Lucia Catani
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	polycythemia vera cancer extracellular vesicles microbial DNA cargo gut microbiota Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/930c8ce00f8148a0925025c84058523c
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spelling	oai:doaj.org-article:930c8ce00f8148a0925025c84058523c2021-11-30T21:00:50ZAn Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera2234-943X10.3389/fonc.2021.715217https://doaj.org/article/930c8ce00f8148a0925025c84058523c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.715217/fullhttps://doaj.org/toc/2234-943XPolycythemia Vera (PV) is a myeloproliferative neoplasm with increased risk of thrombosis and progression to myelofibrosis. Chronic inflammation is commonly observed in myeloproliferative neoplasms including PV. The inflammatory network includes the extracellular vesicles (EVs), which play a role in cell-cell communication. Recent evidence points to circulating microbial components/microbes as potential players in hemopoiesis regulation. To address the role of EVs in PV, here we investigated phenotype and microbial DNA cargo of circulating EVs through multidimensional analysis. Peripheral blood and feces were collected from PV patients (n=38) and healthy donors (n=30). Circulating megakaryocyte (MK)- and platelet (PLT)-derived EVs were analyzed by flow cytometry. After microbial DNA extraction from feces and isolated EVs, the 16S rDNA V3-V4 region was sequenced. We found that the proportion of circulating MK-derived EVs was significantly decreased in PV patients as compared with the healthy donors. By contrast, the proportion of the PLT-derived EVs was increased. Interestingly, PV was also associated with a microbial DNA signature of the isolated EVs with higher diversity and distinct microbial composition than the healthy counterparts. Of note, increased proportion of isolated lipopolysaccharide-associated EVs has been demonstrated in PV patients. Conversely, the gut microbiome profile failed to identify a distinct layout between PV patients and healthy donors. In conclusion, PV is associated with circulating EVs harbouring abnormal phenotype and dysbiosis signature with a potential role in the (inflammatory) pathogenesis of the disease.Monica BaroneMonica BaroneMartina BaroneFrancesca RicciGiuseppe AuteriGiulia CorradiFrancesco FabbriValentina PapaErika BandiniGiovanna CenacchiPier Luigi TazzariNicola VianelliSilvia TurroniMichele CavoFrancesca PalandriMarco CandelaLucia CataniFrontiers Media S.A.articlepolycythemia veracancerextracellular vesiclesmicrobial DNA cargogut microbiotaNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polycythemia vera cancer extracellular vesicles microbial DNA cargo gut microbiota Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	polycythemia vera cancer extracellular vesicles microbial DNA cargo gut microbiota Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Monica Barone Monica Barone Martina Barone Francesca Ricci Giuseppe Auteri Giulia Corradi Francesco Fabbri Valentina Papa Erika Bandini Giovanna Cenacchi Pier Luigi Tazzari Nicola Vianelli Silvia Turroni Michele Cavo Francesca Palandri Marco Candela Lucia Catani An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
description	Polycythemia Vera (PV) is a myeloproliferative neoplasm with increased risk of thrombosis and progression to myelofibrosis. Chronic inflammation is commonly observed in myeloproliferative neoplasms including PV. The inflammatory network includes the extracellular vesicles (EVs), which play a role in cell-cell communication. Recent evidence points to circulating microbial components/microbes as potential players in hemopoiesis regulation. To address the role of EVs in PV, here we investigated phenotype and microbial DNA cargo of circulating EVs through multidimensional analysis. Peripheral blood and feces were collected from PV patients (n=38) and healthy donors (n=30). Circulating megakaryocyte (MK)- and platelet (PLT)-derived EVs were analyzed by flow cytometry. After microbial DNA extraction from feces and isolated EVs, the 16S rDNA V3-V4 region was sequenced. We found that the proportion of circulating MK-derived EVs was significantly decreased in PV patients as compared with the healthy donors. By contrast, the proportion of the PLT-derived EVs was increased. Interestingly, PV was also associated with a microbial DNA signature of the isolated EVs with higher diversity and distinct microbial composition than the healthy counterparts. Of note, increased proportion of isolated lipopolysaccharide-associated EVs has been demonstrated in PV patients. Conversely, the gut microbiome profile failed to identify a distinct layout between PV patients and healthy donors. In conclusion, PV is associated with circulating EVs harbouring abnormal phenotype and dysbiosis signature with a potential role in the (inflammatory) pathogenesis of the disease.
format	article
author	Monica Barone Monica Barone Martina Barone Francesca Ricci Giuseppe Auteri Giulia Corradi Francesco Fabbri Valentina Papa Erika Bandini Giovanna Cenacchi Pier Luigi Tazzari Nicola Vianelli Silvia Turroni Michele Cavo Francesca Palandri Marco Candela Lucia Catani
author_facet	Monica Barone Monica Barone Martina Barone Francesca Ricci Giuseppe Auteri Giulia Corradi Francesco Fabbri Valentina Papa Erika Bandini Giovanna Cenacchi Pier Luigi Tazzari Nicola Vianelli Silvia Turroni Michele Cavo Francesca Palandri Marco Candela Lucia Catani
author_sort	Monica Barone
title	An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title_short	An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title_full	An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title_fullStr	An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title_full_unstemmed	An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title_sort	abnormal host/microbiomes signature of plasma-derived extracellular vesicles is associated to polycythemia vera
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/930c8ce00f8148a0925025c84058523c
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An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera

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