Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.

<h4>Background</h4>In order to identify an inexpensive yet highly stable SARS-CoV-2 collection device as an alternative to foam swabs stored in transport media, both contrived ("surrogate") CoV-positive and patient-collected spun polyester swabs stored in dry tubes were evaluat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Leah R Padgett, Lauren A Kennington, Charlotte L Ahls, Delini K Samarasinghe, Yuan-Po Tu, Michelle L Wallander, Shawna D Cooper, James S Elliott, Douglas Rains
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/930e5bb787274f5b985a1e45c269ebd5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:930e5bb787274f5b985a1e45c269ebd5
record_format	dspace
spelling	oai:doaj.org-article:930e5bb787274f5b985a1e45c269ebd52021-12-02T20:18:55ZPolyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.1932-620310.1371/journal.pone.0245423https://doaj.org/article/930e5bb787274f5b985a1e45c269ebd52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0245423https://doaj.org/toc/1932-6203<h4>Background</h4>In order to identify an inexpensive yet highly stable SARS-CoV-2 collection device as an alternative to foam swabs stored in transport media, both contrived ("surrogate") CoV-positive and patient-collected spun polyester swabs stored in dry tubes were evaluated for time- and temperature-stability using qPCR.<h4>Methods</h4>Surrogate specimens were prepared by combining multiple, residual SARS-CoV-2-positive clinical specimens and diluting to near-LOD levels in either porcine or human mucus ("matrix"), inoculating foam or polyester nasal swabs, and sealing in dry tubes. Swabs were then subjected to one of three temperature excursions: (1) 4°C for up to 72 hours; (2) 40°C for 12 hours, followed by 32°C for up to 60 hours; or (3) multiple freeze-thaw cycles (-20°C). The stability of extracted SARS-CoV-2 RNA for each condition was evaluated by qPCR. Separate usability studies for the dry polyester swab-based HealthPulse@home COVID-19 Specimen Collection Kit were later conducted in both adult and pediatric populations.<h4>Results</h4>Polyester swabs stored dry demonstrated equivalent performance to foam swabs for detection of low and moderate SARS-CoV-2 viral loads. Mimicking warm- and cold- climate shipment, surrogate specimens were stable following either 72 hours of a high-temperature excursion or two freeze-thaw cycles. In addition, usability studies comprised of self-collected patient specimens yielded sufficient material for molecular testing, as demonstrated by RNase P detection.<h4>Conclusions</h4>Polyester nasal swabs stored in dry collection tubes offer a robust and inexpensive self-collection method for SARS-CoV-2 viral load testing, as viral RNA remains stable under conditions required for home collection and shipment to the laboratory.Leah R PadgettLauren A KenningtonCharlotte L AhlsDelini K SamarasingheYuan-Po TuMichelle L WallanderShawna D CooperJames S ElliottDouglas RainsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 4, p e0245423 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leah R Padgett Lauren A Kennington Charlotte L Ahls Delini K Samarasinghe Yuan-Po Tu Michelle L Wallander Shawna D Cooper James S Elliott Douglas Rains Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.
description	<h4>Background</h4>In order to identify an inexpensive yet highly stable SARS-CoV-2 collection device as an alternative to foam swabs stored in transport media, both contrived ("surrogate") CoV-positive and patient-collected spun polyester swabs stored in dry tubes were evaluated for time- and temperature-stability using qPCR.<h4>Methods</h4>Surrogate specimens were prepared by combining multiple, residual SARS-CoV-2-positive clinical specimens and diluting to near-LOD levels in either porcine or human mucus ("matrix"), inoculating foam or polyester nasal swabs, and sealing in dry tubes. Swabs were then subjected to one of three temperature excursions: (1) 4°C for up to 72 hours; (2) 40°C for 12 hours, followed by 32°C for up to 60 hours; or (3) multiple freeze-thaw cycles (-20°C). The stability of extracted SARS-CoV-2 RNA for each condition was evaluated by qPCR. Separate usability studies for the dry polyester swab-based HealthPulse@home COVID-19 Specimen Collection Kit were later conducted in both adult and pediatric populations.<h4>Results</h4>Polyester swabs stored dry demonstrated equivalent performance to foam swabs for detection of low and moderate SARS-CoV-2 viral loads. Mimicking warm- and cold- climate shipment, surrogate specimens were stable following either 72 hours of a high-temperature excursion or two freeze-thaw cycles. In addition, usability studies comprised of self-collected patient specimens yielded sufficient material for molecular testing, as demonstrated by RNase P detection.<h4>Conclusions</h4>Polyester nasal swabs stored in dry collection tubes offer a robust and inexpensive self-collection method for SARS-CoV-2 viral load testing, as viral RNA remains stable under conditions required for home collection and shipment to the laboratory.
format	article
author	Leah R Padgett Lauren A Kennington Charlotte L Ahls Delini K Samarasinghe Yuan-Po Tu Michelle L Wallander Shawna D Cooper James S Elliott Douglas Rains
author_facet	Leah R Padgett Lauren A Kennington Charlotte L Ahls Delini K Samarasinghe Yuan-Po Tu Michelle L Wallander Shawna D Cooper James S Elliott Douglas Rains
author_sort	Leah R Padgett
title	Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.
title_short	Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.
title_full	Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.
title_fullStr	Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.
title_full_unstemmed	Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.
title_sort	polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for sars-cov-2 testing.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/930e5bb787274f5b985a1e45c269ebd5
work_keys_str_mv	AT leahrpadgett polyesternasalswabscollectedinadrytubearearobustandinexpensiveminimalselfcollectionkitforsarscov2testing AT laurenakennington polyesternasalswabscollectedinadrytubearearobustandinexpensiveminimalselfcollectionkitforsarscov2testing AT charlottelahls polyesternasalswabscollectedinadrytubearearobustandinexpensiveminimalselfcollectionkitforsarscov2testing AT deliniksamarasinghe polyesternasalswabscollectedinadrytubearearobustandinexpensiveminimalselfcollectionkitforsarscov2testing AT yuanpotu polyesternasalswabscollectedinadrytubearearobustandinexpensiveminimalselfcollectionkitforsarscov2testing AT michellelwallander polyesternasalswabscollectedinadrytubearearobustandinexpensiveminimalselfcollectionkitforsarscov2testing AT shawnadcooper polyesternasalswabscollectedinadrytubearearobustandinexpensiveminimalselfcollectionkitforsarscov2testing AT jamesselliott polyesternasalswabscollectedinadrytubearearobustandinexpensiveminimalselfcollectionkitforsarscov2testing AT douglasrains polyesternasalswabscollectedinadrytubearearobustandinexpensiveminimalselfcollectionkitforsarscov2testing
_version_	1718374241481523200

Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.

Ejemplares similares