The Chorion Proteome of <i>Diaphorina citri</i>, the Vector of Huanglongbing Disease in Citrus
Nowadays, the Asian citrus psyllid, <i>Diaphorina citri</i> (Kuwayama) (Hemiptera: Liviidae) is considered the most devastating pest of citrus because it transmits “<i>Candidatus</i> Liberibacter asiaticus”, the putative causal agent of huanglongbing (HLB) or citrus greening....
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| Autores principales: | , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/930eff38bf5f41bf86d31fb885ae233c |
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| Sumario: | Nowadays, the Asian citrus psyllid, <i>Diaphorina citri</i> (Kuwayama) (Hemiptera: Liviidae) is considered the most devastating pest of citrus because it transmits “<i>Candidatus</i> Liberibacter asiaticus”, the putative causal agent of huanglongbing (HLB) or citrus greening. Controlling the vector is the main strategy used to mitigate HLB. Targeting <i>D. citri</i> at the very early stages of its development may offer an effective control strategy. Identifying chorion proteins will contribute to a better understanding of embryo development and egg hatching and thus could lead to valuable targets to better control psyllid populations. Herein, we analyze the chorion proteins of <i>D. citri.</i> Mass spectrometry-based bottom-up/shotgun proteomics and databases were queried to achieve protein identification. Fifty-one proteins were identified in <i>D. citri</i> chorion. The <i>D. citri</i> chorion proteins were divided into eight categories according to their biological or molecular function: <i>i—</i>enzymes (25%); <i>ii—</i>binding proteins (10%); <i>iii—</i>structural proteins (8%); <i>iv—</i>homeostasis-related proteins, mostly vitellogenins (8%); <i>v—</i>proteins related to gene expression (6%); <i>vi—</i>immune system proteins (6%); <i>vii—</i>other proteins (16%); and <i>viii—</i>uncharacterized proteins (21%). The composition of the chorion proteome suggested that the hatching rate could be reduced by silencing chorion-related genes. The proteomic analysis of <i>D. citri</i> chorion tissue allowed us to identify its proteins, providing promising new targets for <i>D. citri</i> control through RNA interference technology. |
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