The Chorion Proteome of Diaphorina citri, the Vector of Huanglongbing Disease in Citrus

Nowadays, the Asian citrus psyllid, Diaphorina citri (Kuwayama) (Hemiptera: Liviidae) is considered the most devastating pest of citrus because it transmits “Candidatus Liberibacter asiaticus”, the putative causal agent of huanglongbing (HLB) or citrus greening....

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Autores principales:	Yulica Santos-Ortega, Nabil Killiny
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chorion hatching proteomics <i>Diaphorina</i> <i>citri</i> LC-MS/MS Science Q
Acceso en línea:	https://doaj.org/article/930eff38bf5f41bf86d31fb885ae233c
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spelling	oai:doaj.org-article:930eff38bf5f41bf86d31fb885ae233c2021-11-25T17:59:04ZThe Chorion Proteome of <i>Diaphorina citri</i>, the Vector of Huanglongbing Disease in Citrus10.3390/insects121109592075-4450https://doaj.org/article/930eff38bf5f41bf86d31fb885ae233c2021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4450/12/11/959https://doaj.org/toc/2075-4450Nowadays, the Asian citrus psyllid, <i>Diaphorina citri</i> (Kuwayama) (Hemiptera: Liviidae) is considered the most devastating pest of citrus because it transmits “<i>Candidatus</i> Liberibacter asiaticus”, the putative causal agent of huanglongbing (HLB) or citrus greening. Controlling the vector is the main strategy used to mitigate HLB. Targeting <i>D. citri</i> at the very early stages of its development may offer an effective control strategy. Identifying chorion proteins will contribute to a better understanding of embryo development and egg hatching and thus could lead to valuable targets to better control psyllid populations. Herein, we analyze the chorion proteins of <i>D. citri.</i> Mass spectrometry-based bottom-up/shotgun proteomics and databases were queried to achieve protein identification. Fifty-one proteins were identified in <i>D. citri</i> chorion. The <i>D. citri</i> chorion proteins were divided into eight categories according to their biological or molecular function: <i>i—</i>enzymes (25%); <i>ii—</i>binding proteins (10%); <i>iii—</i>structural proteins (8%); <i>iv—</i>homeostasis-related proteins, mostly vitellogenins (8%); <i>v—</i>proteins related to gene expression (6%); <i>vi—</i>immune system proteins (6%); <i>vii—</i>other proteins (16%); and <i>viii—</i>uncharacterized proteins (21%). The composition of the chorion proteome suggested that the hatching rate could be reduced by silencing chorion-related genes. The proteomic analysis of <i>D. citri</i> chorion tissue allowed us to identify its proteins, providing promising new targets for <i>D. citri</i> control through RNA interference technology.Yulica Santos-OrtegaNabil KillinyMDPI AGarticlechorionhatchingproteomics<i>Diaphorina</i> <i>citri</i>LC-MS/MSScienceQENInsects, Vol 12, Iss 959, p 959 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chorion hatching proteomics <i>Diaphorina</i> <i>citri</i> LC-MS/MS Science Q
spellingShingle	chorion hatching proteomics <i>Diaphorina</i> <i>citri</i> LC-MS/MS Science Q Yulica Santos-Ortega Nabil Killiny The Chorion Proteome of <i>Diaphorina citri</i>, the Vector of Huanglongbing Disease in Citrus
description	Nowadays, the Asian citrus psyllid, <i>Diaphorina citri</i> (Kuwayama) (Hemiptera: Liviidae) is considered the most devastating pest of citrus because it transmits “<i>Candidatus</i> Liberibacter asiaticus”, the putative causal agent of huanglongbing (HLB) or citrus greening. Controlling the vector is the main strategy used to mitigate HLB. Targeting <i>D. citri</i> at the very early stages of its development may offer an effective control strategy. Identifying chorion proteins will contribute to a better understanding of embryo development and egg hatching and thus could lead to valuable targets to better control psyllid populations. Herein, we analyze the chorion proteins of <i>D. citri.</i> Mass spectrometry-based bottom-up/shotgun proteomics and databases were queried to achieve protein identification. Fifty-one proteins were identified in <i>D. citri</i> chorion. The <i>D. citri</i> chorion proteins were divided into eight categories according to their biological or molecular function: <i>i—</i>enzymes (25%); <i>ii—</i>binding proteins (10%); <i>iii—</i>structural proteins (8%); <i>iv—</i>homeostasis-related proteins, mostly vitellogenins (8%); <i>v—</i>proteins related to gene expression (6%); <i>vi—</i>immune system proteins (6%); <i>vii—</i>other proteins (16%); and <i>viii—</i>uncharacterized proteins (21%). The composition of the chorion proteome suggested that the hatching rate could be reduced by silencing chorion-related genes. The proteomic analysis of <i>D. citri</i> chorion tissue allowed us to identify its proteins, providing promising new targets for <i>D. citri</i> control through RNA interference technology.
format	article
author	Yulica Santos-Ortega Nabil Killiny
author_facet	Yulica Santos-Ortega Nabil Killiny
author_sort	Yulica Santos-Ortega
title	The Chorion Proteome of <i>Diaphorina citri</i>, the Vector of Huanglongbing Disease in Citrus
title_short	The Chorion Proteome of <i>Diaphorina citri</i>, the Vector of Huanglongbing Disease in Citrus
title_full	The Chorion Proteome of <i>Diaphorina citri</i>, the Vector of Huanglongbing Disease in Citrus
title_fullStr	The Chorion Proteome of <i>Diaphorina citri</i>, the Vector of Huanglongbing Disease in Citrus
title_full_unstemmed	The Chorion Proteome of <i>Diaphorina citri</i>, the Vector of Huanglongbing Disease in Citrus
title_sort	chorion proteome of <i>diaphorina citri</i>, the vector of huanglongbing disease in citrus
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/930eff38bf5f41bf86d31fb885ae233c
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The Chorion Proteome of <i>Diaphorina citri</i>, the Vector of Huanglongbing Disease in Citrus

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