Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin

Abstract RNA-seq has matured and become an important tool for studying RNA biology. Here we compared two RNA-seq (MGI DNBSEQ and Illumina NextSeq 500) and two microarray platforms (GeneChip Human Transcriptome Array 2.0 and Illumina Expression BeadChip) in healthy individuals administered recombinan...

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Autores principales:	Guan Wang, Traci Kitaoka, Ali Crawford, Qian Mao, Andrew Hesketh, Fergus M. Guppy, Garrett I. Ash, Jason Liu, Mark B. Gerstein, Yannis P. Pitsiladis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/931896334694467487e08cc5a16a1d1c
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spelling	oai:doaj.org-article:931896334694467487e08cc5a16a1d1c2021-11-08T10:53:28ZCross-platform transcriptomic profiling of the response to recombinant human erythropoietin10.1038/s41598-021-00608-92045-2322https://doaj.org/article/931896334694467487e08cc5a16a1d1c2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00608-9https://doaj.org/toc/2045-2322Abstract RNA-seq has matured and become an important tool for studying RNA biology. Here we compared two RNA-seq (MGI DNBSEQ and Illumina NextSeq 500) and two microarray platforms (GeneChip Human Transcriptome Array 2.0 and Illumina Expression BeadChip) in healthy individuals administered recombinant human erythropoietin for transcriptome-wide quantification of differential gene expression. The results show that total RNA DNB-seq generated a multitude of target genes compared to other platforms. Pathway enrichment analyses revealed genes correlate to not only erythropoiesis and oxygen transport but also a wide range of other functions, such as tissue protection and immune regulation. This study provides a knowledge base of genes relevant to EPO biology through cross-platform comparisons and validation.Guan WangTraci KitaokaAli CrawfordQian MaoAndrew HeskethFergus M. GuppyGarrett I. AshJason LiuMark B. GersteinYannis P. PitsiladisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guan Wang Traci Kitaoka Ali Crawford Qian Mao Andrew Hesketh Fergus M. Guppy Garrett I. Ash Jason Liu Mark B. Gerstein Yannis P. Pitsiladis Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin
description	Abstract RNA-seq has matured and become an important tool for studying RNA biology. Here we compared two RNA-seq (MGI DNBSEQ and Illumina NextSeq 500) and two microarray platforms (GeneChip Human Transcriptome Array 2.0 and Illumina Expression BeadChip) in healthy individuals administered recombinant human erythropoietin for transcriptome-wide quantification of differential gene expression. The results show that total RNA DNB-seq generated a multitude of target genes compared to other platforms. Pathway enrichment analyses revealed genes correlate to not only erythropoiesis and oxygen transport but also a wide range of other functions, such as tissue protection and immune regulation. This study provides a knowledge base of genes relevant to EPO biology through cross-platform comparisons and validation.
format	article
author	Guan Wang Traci Kitaoka Ali Crawford Qian Mao Andrew Hesketh Fergus M. Guppy Garrett I. Ash Jason Liu Mark B. Gerstein Yannis P. Pitsiladis
author_facet	Guan Wang Traci Kitaoka Ali Crawford Qian Mao Andrew Hesketh Fergus M. Guppy Garrett I. Ash Jason Liu Mark B. Gerstein Yannis P. Pitsiladis
author_sort	Guan Wang
title	Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin
title_short	Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin
title_full	Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin
title_fullStr	Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin
title_full_unstemmed	Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin
title_sort	cross-platform transcriptomic profiling of the response to recombinant human erythropoietin
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/931896334694467487e08cc5a16a1d1c
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Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin

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