Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes

Endometriosis (EMS) pathogenesis has been related to the release of inflammatory mediators in peritoneal fluid, creating an altered microenvironment that leads to low-grade oocyte/embryos and to the reduction of implantation rates. The Epithelial–Mesenchymal Transition (EMT), an inflammation-related...

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Autores principales:	Vito Cela, Elisa Malacarne, Maria Elena Rosa Obino, Ilaria Marzi, Francesca Papini, Francesca Vergine, Elena Pisacreta, Elisa Zappelli, Deborah Pietrobono, Giorgia Scarfò, Simona Daniele, Ferdinando Franzoni, Claudia Martini, Paolo Giovanni Artini
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	endometriosis follicular fluid inflammatory cytokines EMT Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/931de39c93ed4b37be56c0d12d4ff188
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spelling	oai:doaj.org-article:931de39c93ed4b37be56c0d12d4ff1882021-11-25T16:50:45ZExploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes10.3390/biomedicines91116812227-9059https://doaj.org/article/931de39c93ed4b37be56c0d12d4ff1882021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1681https://doaj.org/toc/2227-9059Endometriosis (EMS) pathogenesis has been related to the release of inflammatory mediators in peritoneal fluid, creating an altered microenvironment that leads to low-grade oocyte/embryos and to the reduction of implantation rates. The Epithelial–Mesenchymal Transition (EMT), an inflammation-related process, can be a further contributing factor to EMS. This study aimed to investigate, among various cytokines and EMT markers (Cadherins, TGF-β, HIF-1α), diagnostic markers of EMS and prognostic factors of in vitro fertilization (IVF) outcomes. Herein, EMS patients manifested higher serum levels of the inflammatory molecules IL-6, IL-8, and IL-12 and a decrease in the concentrations of the anti-inflammatory IL-10. Moreover, biochemical markers associated with the EMT process were more elevated in serum and follicular fluid (FF) of EMS patients than in controls. At the end, the number of good-quality embryos was inversely related to serum IL-6 and EMT markers. Interestingly, serum IL-6 and FF IL-10 concentrations differentiated EMS patients from controls. Finally, serum IL-8 and E-Cadherin levels, as well as FF IL-10, predicted positive IVF outcome with great accuracy. Our data confirm the pivotal role of inflammatory mediators (i.e., IL-6 and IL-10) in EMS pathogenesis and suggest that EMT-related markers are elevated in EMS patients and can be predictive of IVF outcome.Vito CelaElisa MalacarneMaria Elena Rosa ObinoIlaria MarziFrancesca PapiniFrancesca VergineElena PisacretaElisa ZappelliDeborah PietrobonoGiorgia ScarfòSimona DanieleFerdinando FranzoniClaudia MartiniPaolo Giovanni ArtiniMDPI AGarticleendometriosisfollicular fluidinflammatory cytokinesEMTBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1681, p 1681 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endometriosis follicular fluid inflammatory cytokines EMT Biology (General) QH301-705.5
spellingShingle	endometriosis follicular fluid inflammatory cytokines EMT Biology (General) QH301-705.5 Vito Cela Elisa Malacarne Maria Elena Rosa Obino Ilaria Marzi Francesca Papini Francesca Vergine Elena Pisacreta Elisa Zappelli Deborah Pietrobono Giorgia Scarfò Simona Daniele Ferdinando Franzoni Claudia Martini Paolo Giovanni Artini Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes
description	Endometriosis (EMS) pathogenesis has been related to the release of inflammatory mediators in peritoneal fluid, creating an altered microenvironment that leads to low-grade oocyte/embryos and to the reduction of implantation rates. The Epithelial–Mesenchymal Transition (EMT), an inflammation-related process, can be a further contributing factor to EMS. This study aimed to investigate, among various cytokines and EMT markers (Cadherins, TGF-β, HIF-1α), diagnostic markers of EMS and prognostic factors of in vitro fertilization (IVF) outcomes. Herein, EMS patients manifested higher serum levels of the inflammatory molecules IL-6, IL-8, and IL-12 and a decrease in the concentrations of the anti-inflammatory IL-10. Moreover, biochemical markers associated with the EMT process were more elevated in serum and follicular fluid (FF) of EMS patients than in controls. At the end, the number of good-quality embryos was inversely related to serum IL-6 and EMT markers. Interestingly, serum IL-6 and FF IL-10 concentrations differentiated EMS patients from controls. Finally, serum IL-8 and E-Cadherin levels, as well as FF IL-10, predicted positive IVF outcome with great accuracy. Our data confirm the pivotal role of inflammatory mediators (i.e., IL-6 and IL-10) in EMS pathogenesis and suggest that EMT-related markers are elevated in EMS patients and can be predictive of IVF outcome.
format	article
author	Vito Cela Elisa Malacarne Maria Elena Rosa Obino Ilaria Marzi Francesca Papini Francesca Vergine Elena Pisacreta Elisa Zappelli Deborah Pietrobono Giorgia Scarfò Simona Daniele Ferdinando Franzoni Claudia Martini Paolo Giovanni Artini
author_facet	Vito Cela Elisa Malacarne Maria Elena Rosa Obino Ilaria Marzi Francesca Papini Francesca Vergine Elena Pisacreta Elisa Zappelli Deborah Pietrobono Giorgia Scarfò Simona Daniele Ferdinando Franzoni Claudia Martini Paolo Giovanni Artini
author_sort	Vito Cela
title	Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes
title_short	Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes
title_full	Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes
title_fullStr	Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes
title_full_unstemmed	Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes
title_sort	exploring epithelial–mesenchymal transition signals in endometriosis diagnosis and in vitro fertilization outcomes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/931de39c93ed4b37be56c0d12d4ff188
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Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes

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