Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity
Nanometer-scale properties of the extracellular matrix influence many biological processes, including cell motility. While much information is available for single-cell migration, to date, no knowledge exists on how the nanoscale presentation of extracellular matrix receptors influences collective c...
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eLife Sciences Publications Ltd
2021
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oai:doaj.org-article:932081bb1fa54e35a976399760cd037f2021-11-30T10:04:48ZIntegrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity10.7554/eLife.698612050-084Xe69861https://doaj.org/article/932081bb1fa54e35a976399760cd037f2021-09-01T00:00:00Zhttps://elifesciences.org/articles/69861https://doaj.org/toc/2050-084XNanometer-scale properties of the extracellular matrix influence many biological processes, including cell motility. While much information is available for single-cell migration, to date, no knowledge exists on how the nanoscale presentation of extracellular matrix receptors influences collective cell migration. In wound healing, basal keratinocytes collectively migrate on a fibronectin-rich provisional basement membrane to re-epithelialize the injured skin. Among other receptors, the fibronectin receptor integrin α5β1 plays a pivotal role in this process. Using a highly specific integrin α5β1 peptidomimetic combined with nanopatterned hydrogels, we show that keratinocyte sheets regulate their migration ability at an optimal integrin α5β1 nanospacing. This efficiency relies on the effective propagation of stresses within the cell monolayer independent of substrate stiffness. For the first time, this work highlights the importance of extracellular matrix receptor nanoscale organization required for efficient tissue regeneration.Jacopo Di RussoJennifer L YoungJulian WR WegnerTimmy SteinsHorst KesslerJoachim P SpatzeLife Sciences Publications Ltdarticlewound healingkeratocytesintegrin α5β1collective cell migrationMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021) |
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wound healing keratocytes integrin α5β1 collective cell migration Medicine R Science Q Biology (General) QH301-705.5 |
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wound healing keratocytes integrin α5β1 collective cell migration Medicine R Science Q Biology (General) QH301-705.5 Jacopo Di Russo Jennifer L Young Julian WR Wegner Timmy Steins Horst Kessler Joachim P Spatz Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity |
| description |
Nanometer-scale properties of the extracellular matrix influence many biological processes, including cell motility. While much information is available for single-cell migration, to date, no knowledge exists on how the nanoscale presentation of extracellular matrix receptors influences collective cell migration. In wound healing, basal keratinocytes collectively migrate on a fibronectin-rich provisional basement membrane to re-epithelialize the injured skin. Among other receptors, the fibronectin receptor integrin α5β1 plays a pivotal role in this process. Using a highly specific integrin α5β1 peptidomimetic combined with nanopatterned hydrogels, we show that keratinocyte sheets regulate their migration ability at an optimal integrin α5β1 nanospacing. This efficiency relies on the effective propagation of stresses within the cell monolayer independent of substrate stiffness. For the first time, this work highlights the importance of extracellular matrix receptor nanoscale organization required for efficient tissue regeneration. |
| format |
article |
| author |
Jacopo Di Russo Jennifer L Young Julian WR Wegner Timmy Steins Horst Kessler Joachim P Spatz |
| author_facet |
Jacopo Di Russo Jennifer L Young Julian WR Wegner Timmy Steins Horst Kessler Joachim P Spatz |
| author_sort |
Jacopo Di Russo |
| title |
Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity |
| title_short |
Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity |
| title_full |
Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity |
| title_fullStr |
Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity |
| title_full_unstemmed |
Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity |
| title_sort |
integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity |
| publisher |
eLife Sciences Publications Ltd |
| publishDate |
2021 |
| url |
https://doaj.org/article/932081bb1fa54e35a976399760cd037f |
| work_keys_str_mv |
AT jacopodirusso integrina5b1nanopresentationregulatescollectivekeratinocytemigrationindependentofsubstraterigidity AT jenniferlyoung integrina5b1nanopresentationregulatescollectivekeratinocytemigrationindependentofsubstraterigidity AT julianwrwegner integrina5b1nanopresentationregulatescollectivekeratinocytemigrationindependentofsubstraterigidity AT timmysteins integrina5b1nanopresentationregulatescollectivekeratinocytemigrationindependentofsubstraterigidity AT horstkessler integrina5b1nanopresentationregulatescollectivekeratinocytemigrationindependentofsubstraterigidity AT joachimpspatz integrina5b1nanopresentationregulatescollectivekeratinocytemigrationindependentofsubstraterigidity |
| _version_ |
1718406698336518144 |