Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.

<h4>Background</h4>Infections by A. calcoaceticus-A. baumannii (ACB) complex isolates represent a serious threat for wounded and burn patients. Three international multidrug-resistant (MDR) clones (EU clone I-III) are responsible for a large proportion of nosocomial infections with A. ba...

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Autores principales:	Yolande Hauck, Charles Soler, Patrick Jault, Audrey Mérens, Patrick Gérome, Christine Mac Nab, François Trueba, Laurent Bargues, Hoang Vu Thien, Gilles Vergnaud, Christine Pourcel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9327f94584464b02acb43f7944054e96
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spelling	oai:doaj.org-article:9327f94584464b02acb43f7944054e962021-11-18T07:05:51ZDiversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.1932-620310.1371/journal.pone.0044597https://doaj.org/article/9327f94584464b02acb43f7944054e962012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22984530/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Infections by A. calcoaceticus-A. baumannii (ACB) complex isolates represent a serious threat for wounded and burn patients. Three international multidrug-resistant (MDR) clones (EU clone I-III) are responsible for a large proportion of nosocomial infections with A. baumannii but other emerging strains with high epidemic potential also occur.<h4>Methodology/principal findings</h4>We automatized a Multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) protocol and used it to investigate the genetic diversity of 136 ACB isolates from four military hospitals and one childrens hospital. Acinetobacter sp other than baumannii isolates represented 22.6% (31/137) with a majority being A. pittii. The genotyping protocol designed for A.baumannii was also efficient to cluster A. pittii isolates. Fifty-five percent of A. baumannii isolates belonged to the two international clones I and II, and we identified new clones which members were found in the different hospitals. Analysis of two CRISPR-cas systems helped define two clonal complexes and provided phylogenetic information to help trace back their emergence.<h4>Conclusions/significance</h4>The increasing occurrence of A. baumannii infections in the hospital calls for measures to rapidly characterize the isolates and identify emerging clones. The automatized MLVA protocol can be the instrument for such surveys. In addition, the investigation of CRISPR/cas systems may give important keys to understand the evolution of some highly successful clonal complexes.Yolande HauckCharles SolerPatrick JaultAudrey MérensPatrick GéromeChristine Mac NabFrançois TruebaLaurent BarguesHoang Vu ThienGilles VergnaudChristine PourcelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e44597 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yolande Hauck Charles Soler Patrick Jault Audrey Mérens Patrick Gérome Christine Mac Nab François Trueba Laurent Bargues Hoang Vu Thien Gilles Vergnaud Christine Pourcel Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.
description	<h4>Background</h4>Infections by A. calcoaceticus-A. baumannii (ACB) complex isolates represent a serious threat for wounded and burn patients. Three international multidrug-resistant (MDR) clones (EU clone I-III) are responsible for a large proportion of nosocomial infections with A. baumannii but other emerging strains with high epidemic potential also occur.<h4>Methodology/principal findings</h4>We automatized a Multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) protocol and used it to investigate the genetic diversity of 136 ACB isolates from four military hospitals and one childrens hospital. Acinetobacter sp other than baumannii isolates represented 22.6% (31/137) with a majority being A. pittii. The genotyping protocol designed for A.baumannii was also efficient to cluster A. pittii isolates. Fifty-five percent of A. baumannii isolates belonged to the two international clones I and II, and we identified new clones which members were found in the different hospitals. Analysis of two CRISPR-cas systems helped define two clonal complexes and provided phylogenetic information to help trace back their emergence.<h4>Conclusions/significance</h4>The increasing occurrence of A. baumannii infections in the hospital calls for measures to rapidly characterize the isolates and identify emerging clones. The automatized MLVA protocol can be the instrument for such surveys. In addition, the investigation of CRISPR/cas systems may give important keys to understand the evolution of some highly successful clonal complexes.
format	article
author	Yolande Hauck Charles Soler Patrick Jault Audrey Mérens Patrick Gérome Christine Mac Nab François Trueba Laurent Bargues Hoang Vu Thien Gilles Vergnaud Christine Pourcel
author_facet	Yolande Hauck Charles Soler Patrick Jault Audrey Mérens Patrick Gérome Christine Mac Nab François Trueba Laurent Bargues Hoang Vu Thien Gilles Vergnaud Christine Pourcel
author_sort	Yolande Hauck
title	Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.
title_short	Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.
title_full	Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.
title_fullStr	Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.
title_full_unstemmed	Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.
title_sort	diversity of acinetobacter baumannii in four french military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9327f94584464b02acb43f7944054e96
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Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.

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