Novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence.

<h4>Objective</h4>To screen for obstructive sleep apnea (OSA) biomarkers, isobaric tags for relative and absolute quantitation (iTRAQ)-labeled quantitative proteomics assay was used to identify differentially expressed proteins (DEPs) during chronic intermittent hypoxia (CIH).<h4>M...

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Autores principales:	Xiaojun Tang, Shisheng Li, Xinming Yang, Qinglai Tang, Ying Zhang, Shiying Zeng, Mengmeng Li, Kang Jiang, Lu Guo, Peiying Huang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/932c6fa4fbe449d985dd7c9fcf8769b5
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spelling	oai:doaj.org-article:932c6fa4fbe449d985dd7c9fcf8769b52021-12-02T20:09:50ZNovel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence.1932-620310.1371/journal.pone.0253943https://doaj.org/article/932c6fa4fbe449d985dd7c9fcf8769b52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253943https://doaj.org/toc/1932-6203<h4>Objective</h4>To screen for obstructive sleep apnea (OSA) biomarkers, isobaric tags for relative and absolute quantitation (iTRAQ)-labeled quantitative proteomics assay was used to identify differentially expressed proteins (DEPs) during chronic intermittent hypoxia (CIH).<h4>Method</h4>The iTRAQ technique was applied to compare DEPs in the serum of a CIH rat model and control group. Biological analysis of DEPs was performed using Gene Ontology and Kyoto Encyclopedia to explore related biological functions and signaling pathways. Enzyme-linked immunosorbent assay (ELISA) was performed to validate their expression in sera from patients with OSA and CIH rats.<h4>Results</h4>Twenty-three DEPs (fold change ≥1.2 or ≤0.833, p<0.05) were identified, and two DEPs (unique peptides>3 and higher coverage) were further verified by ELISA in the CIH rat model and OSA subject: apolipoprotein A-IV (APOA4, p<0.05) and Tubulin alpha-1A chain (TUBA1A, p<0.05). Both groups showed significant differences in the expression levels of DEPs between the CIH and control groups and the severe OSA and non-OSA groups. APOA4 was found to be upregulated and TUBA1A downregulated in both the sera from OSA patients and CIH rats, on comparing proteomics results with clinical results. There were two pathways that involved three DEPs, the mitogen-activated protein kinase (MAPK) signaling pathway (p<0.05) and cytokine-cytokine receptor interaction (p<0.05).<h4>Conclusion</h4>APOA4 and TUBA1A may be potential novel biomarkers for CIH and OSA, and may play an important role in the development of OSA complications.Xiaojun TangShisheng LiXinming YangQinglai TangYing ZhangShiying ZengMengmeng LiKang JiangLu GuoPeiying HuangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253943 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaojun Tang Shisheng Li Xinming Yang Qinglai Tang Ying Zhang Shiying Zeng Mengmeng Li Kang Jiang Lu Guo Peiying Huang Novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence.
description	<h4>Objective</h4>To screen for obstructive sleep apnea (OSA) biomarkers, isobaric tags for relative and absolute quantitation (iTRAQ)-labeled quantitative proteomics assay was used to identify differentially expressed proteins (DEPs) during chronic intermittent hypoxia (CIH).<h4>Method</h4>The iTRAQ technique was applied to compare DEPs in the serum of a CIH rat model and control group. Biological analysis of DEPs was performed using Gene Ontology and Kyoto Encyclopedia to explore related biological functions and signaling pathways. Enzyme-linked immunosorbent assay (ELISA) was performed to validate their expression in sera from patients with OSA and CIH rats.<h4>Results</h4>Twenty-three DEPs (fold change ≥1.2 or ≤0.833, p<0.05) were identified, and two DEPs (unique peptides>3 and higher coverage) were further verified by ELISA in the CIH rat model and OSA subject: apolipoprotein A-IV (APOA4, p<0.05) and Tubulin alpha-1A chain (TUBA1A, p<0.05). Both groups showed significant differences in the expression levels of DEPs between the CIH and control groups and the severe OSA and non-OSA groups. APOA4 was found to be upregulated and TUBA1A downregulated in both the sera from OSA patients and CIH rats, on comparing proteomics results with clinical results. There were two pathways that involved three DEPs, the mitogen-activated protein kinase (MAPK) signaling pathway (p<0.05) and cytokine-cytokine receptor interaction (p<0.05).<h4>Conclusion</h4>APOA4 and TUBA1A may be potential novel biomarkers for CIH and OSA, and may play an important role in the development of OSA complications.
format	article
author	Xiaojun Tang Shisheng Li Xinming Yang Qinglai Tang Ying Zhang Shiying Zeng Mengmeng Li Kang Jiang Lu Guo Peiying Huang
author_facet	Xiaojun Tang Shisheng Li Xinming Yang Qinglai Tang Ying Zhang Shiying Zeng Mengmeng Li Kang Jiang Lu Guo Peiying Huang
author_sort	Xiaojun Tang
title	Novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence.
title_short	Novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence.
title_full	Novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence.
title_fullStr	Novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence.
title_full_unstemmed	Novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence.
title_sort	novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: from rat model to clinical evidence.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/932c6fa4fbe449d985dd7c9fcf8769b5
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Novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence.

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