Platelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the TGFβ signalling pathway

Abstract Autologous fat grafting is a gold standard therapy for soft tissue defects, but is hampered by unpredictable postoperative outcomes. Fat graft enrichment with adipose-derived stromal cell (ASCs) was recently reported to enhance graft survival. Platelet-rich plasma (PRP) has also emerged as...

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Autores principales:	Bérengère Chignon-Sicard, Magali Kouidhi, Xi Yao, Audrey Delerue-Audegond, Phi Villageois, Pascal Peraldi, Patricia Ferrari, Yves Rival, David Piwnica, Jérôme Aubert, Christian Dani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/932ca9fe73ea48d8bbb07ffc6ae4ba71
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spelling	oai:doaj.org-article:932ca9fe73ea48d8bbb07ffc6ae4ba712021-12-02T11:53:09ZPlatelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the TGFβ signalling pathway10.1038/s41598-017-03113-02045-2322https://doaj.org/article/932ca9fe73ea48d8bbb07ffc6ae4ba712017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03113-0https://doaj.org/toc/2045-2322Abstract Autologous fat grafting is a gold standard therapy for soft tissue defects, but is hampered by unpredictable postoperative outcomes. Fat graft enrichment with adipose-derived stromal cell (ASCs) was recently reported to enhance graft survival. Platelet-rich plasma (PRP) has also emerged as a biologic scaffold that promotes fat graft viability. Combined ASC/PRP fat grafting enrichment is thus a promising new regenerative medicine approach. The effects of PRP on ASC proliferation are well documented, but the impact of PRP on ASC differentiation has yet to be investigated in depth to further elucidate the PRP clinical effects. Here we analyzed the human ASC fate upon PRP treatment. PRP was found to sharply reduce the potential of ASCs to undergo differentiation into adipocytes. Interestingly, the PRP anti-adipogenic effect was accompanied by the generation of myofibroblast-like cells. Among the various factors released from PRP, TGFβ pathway activators played a critical role in both the anti-adipogenic and pro-myofibroblastic PRP effects. Overall, these data suggest that PRP participates in maintaining a pool of ASCs and in the repair process by promoting ASC differentiation into myofibroblast-like cells. TGFβ may provide an important target pathway to improve PRP clinical outcomes.Bérengère Chignon-SicardMagali KouidhiXi YaoAudrey Delerue-AudegondPhi VillageoisPascal PeraldiPatricia FerrariYves RivalDavid PiwnicaJérôme AubertChristian DaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bérengère Chignon-Sicard Magali Kouidhi Xi Yao Audrey Delerue-Audegond Phi Villageois Pascal Peraldi Patricia Ferrari Yves Rival David Piwnica Jérôme Aubert Christian Dani Platelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the TGFβ signalling pathway
description	Abstract Autologous fat grafting is a gold standard therapy for soft tissue defects, but is hampered by unpredictable postoperative outcomes. Fat graft enrichment with adipose-derived stromal cell (ASCs) was recently reported to enhance graft survival. Platelet-rich plasma (PRP) has also emerged as a biologic scaffold that promotes fat graft viability. Combined ASC/PRP fat grafting enrichment is thus a promising new regenerative medicine approach. The effects of PRP on ASC proliferation are well documented, but the impact of PRP on ASC differentiation has yet to be investigated in depth to further elucidate the PRP clinical effects. Here we analyzed the human ASC fate upon PRP treatment. PRP was found to sharply reduce the potential of ASCs to undergo differentiation into adipocytes. Interestingly, the PRP anti-adipogenic effect was accompanied by the generation of myofibroblast-like cells. Among the various factors released from PRP, TGFβ pathway activators played a critical role in both the anti-adipogenic and pro-myofibroblastic PRP effects. Overall, these data suggest that PRP participates in maintaining a pool of ASCs and in the repair process by promoting ASC differentiation into myofibroblast-like cells. TGFβ may provide an important target pathway to improve PRP clinical outcomes.
format	article
author	Bérengère Chignon-Sicard Magali Kouidhi Xi Yao Audrey Delerue-Audegond Phi Villageois Pascal Peraldi Patricia Ferrari Yves Rival David Piwnica Jérôme Aubert Christian Dani
author_facet	Bérengère Chignon-Sicard Magali Kouidhi Xi Yao Audrey Delerue-Audegond Phi Villageois Pascal Peraldi Patricia Ferrari Yves Rival David Piwnica Jérôme Aubert Christian Dani
author_sort	Bérengère Chignon-Sicard
title	Platelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the TGFβ signalling pathway
title_short	Platelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the TGFβ signalling pathway
title_full	Platelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the TGFβ signalling pathway
title_fullStr	Platelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the TGFβ signalling pathway
title_full_unstemmed	Platelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the TGFβ signalling pathway
title_sort	platelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the tgfβ signalling pathway
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/932ca9fe73ea48d8bbb07ffc6ae4ba71
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Platelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the TGFβ signalling pathway

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